Objective Exercise has been approved as an effective anti-aging approach. However, how exercise affects the organelle-specific redox status of the endoplasmic reticulum (ER) and whether it contributes to ER function and healthy aging are still unknown.Methods We constructed an ER-specific reductive stress C. elegans model that overexpresses ctl-1, a homolog of the mammalian catalase gene, to research the effect of ER reductive stress on aging at the organismal level. We then used the Hyperion_ER probe which responds well to hydrogen peroxide to evaluate the redox status in the ER of body wall muscle during swimming and during aging.Results Our results show that H₂O₂ in the ER was markedly reduced during aging and the number of body bending, the life span and the stress response ability in Pnfya-1::ctl-1_ER::mCherry C. elegans was markedly decreased compared with that in Pnfya-1::ctl-1-M_ER::mCherry, indicating that ER reductive stress occurs during the aging process and ER reductive stress promotes aging at the organismal level. Both short-term and long-term exercise can increase the oxidative power of the ER in C. elegans, and exercise alleviates the age-related ER reductive stress and promotes healthy aging.Conclusion Our results demonstrate the effect of exercise on ER redox status at the organelle level for the first time and uncover a new mechanism for exercise in delaying aging at the organismal level from the redox point of view, suggesting that maintaining the oxidation power of the ER may be a valuable geroprotective strategy.]]> 2022/3/21 0:00:00 WANG Yuan-Yuan,QIAO Xin-Hua,SHI Chang,YE Ao-Jun,GUO Miao-Miao,ZHAO Yu-Zheng and CHEN Chang 7true +/NADH ratio, and can clearly activate sirtuins. In addition, altered levels of active factors and kinases can also promote gene and protein expression of enzymes and regulate deacylation. Briefly, the regulation of exercise on protein acylation is a new mechanism for exercise that improves metabolism, promotes health and prevents chronic diseases, relevant research work is still in its infancy and deserves special focus.]]> 2022/3/21 0:00:00 HUANG Wen-Hua,ZHANG Jing-Bo,CHEN Xue-Fei and ZHANG Jing 6true Bmal1) is the core gene of biological clock, which belongs to the transcription factor family of bHLH-PAS (basic helix-loop-helix-per-arnt-sim) domain. It can regulate the circadian rhythm through its own expression and the transcription-translation feedback regulation of the biological clock, which plays an important role in the life activities of organism. The disorder of biological clock induces a series of chronic metabolic diseases, such as cardiovascular diseases, hepatopathy and neurodegenerative diseases, usually accompanied by abnormal expression of Bmal1. Exercise may up-regulate Bmal1 expression in peripheral tissues and organs to improve chronic metabolic diseases. Different exercise loads and types of exercise, such as aerobic exercise, resistance exercise, will lead to the differential expression of Bmal1. There are many potential mechanisms of Bmal1 in improving chronic metabolic diseases by exercise intervention, such as reducing inflammation and oxidative stress, regulating autophagy, maintaining mitochondrial quality and function, interacting with exerkines and microRNA. This article reviews the physiological function of Bmal1 in multiple tissues and organs and the relationship between corresponding chronic metabolic diseases, discussing the influence of exercise intervention on Bmal1 expression deeply and putting forward the potential mechanism of Bmal1 in improving chronic metabolic diseases by exercise, in order to provide a new perspective for exercise as a non-drug treatment to prevent and treat chronic metabolic diseases.]]> 2022/3/21 0:00:00 DI Ling-Yun and TIAN Zhen-Jun 5true via non-pharmacological exercise therapy.]]> 2022/3/21 0:00:00 XU Zi-Han and YOU Hao-Jun 4true 2022/3/21 0:00:00 KE Zhi-Fei,SHANG Hua-Yu,LEI Bin-Kai,CAO Chun-Xia,WANG Zhen,WANG Rui-Yuan and LI Jun-Ping 3true 2022/3/21 0:00:00 ZHAO Sha-Sha,SHI Li-Jun and WU Ying 2true etc.) and cytokines (T-PINP, Nkx3.2, Sox9 and Cleaved-caspase-3, etc.) are closely related to the differentiation and function of OB, chondrocytes and OC. Except from the confirmed GPR48, this review tested out the molecular mechanism of GPCRs regulating bone formation and bone resorption and their role in the effect of exercise on bone metabolism. The mechanism of other GPCRs in the improvement of bone metabolism by exercise has yet to be revealed on what kind of exercise mode and intensity can effectively regulate GPCR and improve bone metabolism and so on. The results help to screen out sensitive GPCRs on membranes as “effectors” for drug development of bone metabolic diseases and “star proteins” for mechanical stimulation in exercise intervention and provide more targets or perspectives for the research and prevention of osteoporosis.]]> 2022/3/21 0:00:00 CHEN Xiang-He,LIU Bo,LU Peng-Cheng,QIU Xiao,ZHOU Xiang-Xiang and ZENG Xin-Yu 1true