张守仁,曹立森,沙安莉,周瑞璋,戴杏英 249true 单立新,杜国光 248true 刘训健,屠一锋,程薇,吴忠祥 247true 印螺,丁少宏,肖蕾,冯骆,李玮,王秀琴,吴旻 246true 刘宏志,张志鸿,程极济 245true 沈行良,西川弘恭,(山鸟)本典夫,新谷元章 244true 胡匡祜 243true 翁其亮,万田郞,朱晓明 242true 张志鸿,刘宏志,游永东,汤虹 241true 朱迺硕,顾全珍,徐秋节 240true 夏文乐,周建军,贾锡安,李广元 239true 阎新文,王建一,张苏玲 238true 张志义,臧伦义,徐国瑞 237true 张维波,方雪,刁云程 236true 王大成,陆国光,张季平,谢殿霖,常文瑞,鲁子贤,梁栋材 235true 陈槐卿,钟贵辉,李良,薛振南 234true 刘笑洋,华陵,程振起 233true 李育庆,赵志安,顾其敏 232true 卢景雰 231true 刘磊,郑竺英,姚国正 230true 韩家康,于德山,安荣姝,陈惟昌,高文伟,张力 229true 李晓阳,王厚行 228true 杨福愉,林治焕,沃维汉,杨毛周,王金凤,王淑娅,邢菁如,陈文雯 227true 杨福愉,林治焕,李生广,郭倍奇,应芸书,邢菁如,王淑娅,陈文雯,孙珊,沃维汉,张克,张瑛,史宝生 226true 林政炯,高义贵,宋时英,李军,赵宝光,梁树坚,谢贵福,邹承鲁 225true 刘建宁,杨端,雷克健 224true 吴新,张思和,张添志 223true 张思和,郑竺英 222true 胡匡祜 221true 卢景雰,王金凤,张斌贤 220true 彭程航,邢蕴芳,王丽华,陈小品,王秀英,卞和如,尹殿均 219true 张鸿良 218true 王宝翰,徐京华,李薇 217true 陶芸,金惠生,易莹,顾其敏 216true 区耀华,王志刚,周昕 215true 刘敬忠,高庆生,姜哲,武红,吴冠芸,龙桂芳,梁荣,张锦,邓兵 214true 李钦 213true 孙侖泉,H.Naora,苏成芝 212true 徐洪基,周红奕,胡师学,潘孝仁 211true 申同健,杜杨柱,张贵寅 210true 刘殿功,罗瑞柏,屠亚平,沈七襄,程旺生,汪瑞珠 209true 李良,陈槐卿,钟贵辉,薛振南 208true 汪江淮,王涛,陈惠孙 207true 张遂坡,刘健,吴隽平,徐友涵 206true 曹功杰,冯晓黎,祁国荣 205true 高惠兰,谢大英,杨惠芳,李忠生,左瑾,方福德 204true 胥薇,邓国仁,刘景梅 203true 魏群,Devid Cheng,Ernest Y. C. Lee,Keith Brew 202true 魏群,Dennisse P. Diaz,Tindong Lin,Ernest Y. C. Lee,Luis Echegoyen 201true 何玉凯,张基增,高基民,徐钤 200true 翁元凯,黄山,翁念宇 199true 谭辉玲,色莫代 198true 顾其敏,李育庆,曹凯鸣,忻骅,孙崇荣 197true 李钦,刘奔 196true 祖志飞,韩复生,杨卫民,沈孝宙 195true 张春明,夏德义,杨鸿昌 194true 杨福愉,邢菁如,陈文雯,王淑娅,李秀梅 193true 尹长城,沈子威,赵南明,汤辉仙,贾士荣 192true 张春霆 191true 韩学军,宗建超,孙顺能,俞耀庭 190true 陈焕朝,黄佳康,魏长利,于树玉 189true 王喜萍,刘望夷,王鸣歧 188true 娄艳春,刘望夷,郭裕新 187true 孙汭,田志刚,张捷,崔正言,张建华 186true 许红,张志鸿 185true 王洪涛,刘望夷 184true 曹绍启,吴维,张幼苓,林波海 183true 刘国建,王正国,朱佩芳,周元国,王毅 182true 许平,庄一义,汪俊军 181true 蒋荣庆,娄艳春,刘望夷 180true 陈焕朝,黄佳康,魏长利,于树玉 179true 江丕栋,傅世樒,郑克,马顺福 178true 彭敬,梁云燕,方家椿,牛敏英,石永进,苏虹,赵孟莲,王代树 177true 章净霞,戴维丽,曾宪周,姚蕙英 176true 蒋荣庆,刘望夷 175true 郭刚,解用虹,王维兆,黄健英,刘贤明,牛瑞芳 174true 吴也凡,曾定,林国栋,洪亮,蔡启瑞 173true 吴也凡,曾定,林国栋,洪亮,蔡启瑞 172true 谭辉玲,罗敏,李和平 171true 陈瑞环,劳为德 170true 夏其昌 169true 阎小君,李广元 168true 武须军,李志高,王克勤 167true 赵会全,刘望夷 166true 宋存先,姜耀虹,史瑞文,韩平,王彭延 165true 吴也凡,曾定,洪亮,林国栋 164true 林波海,吴维,曹绍启,张幼苓,傅培云,刘茹兰,朱粉玉,张思和,曲超,石志远,李治湘,赵红 163true 胡匡祜,李璠 162true 黎健,刘清华,蒋雷,王惠君,李健斋,赵满仓,周景林 161true 杨杰,麦荫乔,杨同书 160true 张劲松,刘望夷 159true 张家洵,赵树谦,许士坤,张晶,齐莉,朱一川 158true 阮康成,王洪涛,刘望夷 157true 凌俊,刘望夷,王德宝 156true 胡匡祜,李子孝,苏万芳,姚山麟 155true 徐湘民,马维芳,宋兰林,张基增 154true 沈海法,邵靖宇,B.HAVSTEEN 153true 丛蓉娟,周哲,洪水根,吴钦义 152true 何玉凯,祁国荣 151true 杨安钢,吉昌华,韩骅,杨立宏,苏成芝,许辉,金伯泉 150true 高磊,陈苏民,陈南春,杨安钢,韩骅,刘新平,崔运昌 149true 陈清轩,刘雷,曹颉,宋德秀 148true 王升启,马立人 147true 张劲松,刘望夷 146true 阎小君,徐德忠,肖乐义,苏成芝,吉昌华,陈友绩 145true 郑乐,林南琴,钱嵘,周元聪,阮康成 144true 刘建军,朱正美,崔肇春 143true 孟潞英,张文炳,彭华国 142true 吴震荣,刘文龙 141true 刘爱民,左雄军,陈立仁,李树本 140true 范钰,沈岩,傅四东,林田,赵艳君,吴冠芸 139true 朱元晓,赖春宁,沈倍奋,李慧云 138true 谢玉才,丁怀翌,蔡伟菁,吴春芳,戚文航,龚兰生 137true 任关仁,王大宝,刘一文,陆梅生,潘晓平 136true 林波海,吴维,徐冠群,刘燕,张幼苓,曹绍启 135true 刘德立,禹邦超 134true 王代树,彭敬,梁云燕,方家椿,石永进 133true 金贵昌,张景芝,郑竺英,周桂荣 132true 赵永芳,谢志,谢凤祥,徐宁,曾昭睿,邱红心,舒德新,隋丽英,刘丽娟 131true 朱元晓,赖春宁,王建安,沈倍奋 130true 王大宝,潘晓平,严钱勤,唐峻岭 129true 孟凡寅,方之扬,许红,张志鸿,陈仕明 128true 张劲松,叶敏 127true 涂强,王永俊,李文,谢宝树,朱元晓,阎玉河,余竟源,兰兰,林万明 126true Wen Jinkun,Hu Jing,Qiao Yaming,Zhang Chenhui,Zhou Airu and Tang Jian 125true Wang Liang,Zhang Jinsan,Zhu Dan,Yin Luo,Wang Xiuqin and Wu Min 124true Xu Caimin,Lu Hong,Pan Huazhen and Zhang Zhinan 123true In vivo naked DNA gene transfer method was used in gene therapy of Parkinson's disease (PD). The complex of rat tyrosine hydroxy-lase (TH) expression plasmid and Lipofectin was injected stereotaxically into striatum of PD rat model. The asymmetric rotational behavior was reduced substantially and quickly. On the third day after injection, drug-induced rotation decreased 50% compared with pretreatment scores. Immunohistochemical staining showed TH-positive nerve cells in striatum of injection side, which indicated that TH gene was uptaked and expressed by nerve cells. These preliminary results have general implications for the application of naked DNA transfer techpique in gene therapy of human neurological disease and specific implications for PD.]]> Cao Lei,Zheng Zhongcheng,Liu Xinyuan,Liu Zhenguo,Zhao Yingchun,Chen Shengdi,Jiang Zhihua and Zhou Changfu 122true Wan Qian,Chen Changzheng,Li Boliang and Xia Qichang 121true 2 and C₂H₂ coordination-activation led to the conclusion that the iron-molybdenum cofactor of nitrogenase might be able to give a special treat to its special substrate, i. e. N≡N. The exogenous substrates except N₂ are apparently not to get into the cage of the active-center and / or to manoeuvre as freely as N≡N inside the cage with the proposed structural settings.]]> Liu Aimin,Zhou Taijin,Zhang Hongtu,Wan Huilin and Cai Qirui 120true 12. The number of surface charges shieded by acetylation was adapted as a criterion to calculate the surface charge numbers on both sides of the purple membrane from surface pH 4─11. The result shows that the total surface negative charge numbers are 9 per bacteriorhodopsin at surface pH 5─9 but increases both above surface pH 9 and below surface pH 5. It supports strongly the model based on five divalent cation binding sites on the surface of purple membrane.]]> Wang Guangyu,Hu Kunsheng and Zhang Hengtao 119true E. coli cells in which high level and no luciferase activity were detected, respectively.This result shows that the first sixteen N-terminal amino acids of firefly luciferase involve in catalytic activity.]]> Lu Jianrong,Yang Jian and Jin Zhenhua 118true cis, cis-muconic acid were high. The product was easily separatedfrom enzyme. The immobilized method of extracellular catechol 1,2-dioxygenase was novel and simple. The results presented show that the immobilization of extracellular catechol 1,2-dioxygenase offers an attractive means for the production of cis, cis-muconic acid.]]> Li Li and Li Qin 117true Ji Hong,Huang Tao,Shen Yan and Wu Guanyun 116true Wang Zhiyong,Zheng Zhongcheng,Xu Jingfen and Liu Xinyuan 115true Qin Ling,Ling Jun,Ruan Kangcheng and Liu Wangyi 114true Lan Fenghua,Masato Umeda and Keizo Inoue 113true 2 with Ca^2＋ by fluorescence spectroscopy indicated that Ca^2＋ was very important to BPLA₂.In the prescence of Ca^2＋ ,the environment around the Trp residue in BPLA₂ became more hydrophobic upon the binding of the substrate to the enzyme,resulting in 9 nm blue shift of the Trp fluorescence emission spectrum. But such a phenomenon was not observed in the absence of Ca^2＋.The experiment indicated that there was a hydrophobic region in BPLA₂ molecule. Ca^2＋ might increase the hydrophobility of this region. Further，it was also found that the binding of Ca^2＋ to the enzyme was related to the single His residue of the enzyme.]]> Zheng Le,Feng Bo,Zhou Yuanchong and Ruan Kangcheng 112true E. coli and purified.]]> Chen Yan,Zhang Ying,Li Jinzhao,Deng Wei,Xia Lingchao and Qiu Rong 111true E. coli cytosine deaminase) gene. A human colorectal carcinoma cell line LoVo or a human uterine cervical cancer cell line HeLa was co-transfected with pSV2-neo and PCEA-TK (or PCEA-CD). After selection with G418, the transgenetic cell clones (hoVo/CEA-TK,LoVo/CEA-CD, HeLa/CEA-TK and HeLa/CEA-CD) were obtained. There was no significant difference in either morphology or cell growth curve between suicide gene transduced cells and wild type cells. But LoVo/CEA-TK (or LoVo/CEA-CD) cells were 2000(or 700) times more sensitive to the cytotoxicity of prodrug ganciclovir(or S-Fluorocytosine)than parental LoVo cells. However, HeLa/CEA-TK or HeLa/CEA-CD cells were still resistant to Ganciclovir (or 5-Fluorocytosine). These results showed the possibility of gene therapy for human colorectal carcinoma by cell type-specific expression of suicide genes.]]> Jiang Qiong,Ge Kai,Xu Dehua,Zheng Zhongcheng and Liu Xinyuan 110true Liu Guisheng,Chen Qingxuan,Wang Jing,Wei Qingxin and Chen Yongfu 109true In vitro cleavage experiment showed that R426 transcript was able to cleave the target sequence specifically. Expression experiment indicated that R426 extracted from Bm-N cell can also cleave the target specifically and cause about 30% reduction of BmNPV polyhedra in transfected cell.]]> Zhang Xiaolan,Chen Nongan,Lu Changde and Qi Guorong 108true E.coli strain which could express domain Ⅲ of the extracellular region of the human EGF-R(EGF-RⅢ) in its membrane had been obtained. The results of EGF receptor radiobinding assay showed that ¹²⁵I-EGF could bind specifically to the intact bacterial body, the amount of specific binding varied with the time and temperature of the reaction. The data of Scatchard analysis indicated that the number of the human EGF-RⅢ expressed in the bacteria was about 738 sites/cell, and its dissociation constant was about 3.0×10^-11 mol/L. The sites of the bacteria to which EGF bound were discovered mainly on its membrane by immunoelectronmicroscopy.]]> WANG Yutian,HU Jialu,GAO Lei,CHEN Sumin,YANG Shengli,GONG Yi,FAN Daiming and ZHANG Xueyong 107true E.coli genomic DNA has been constructed. Screening the library with the Era protein probe labeled by digoxigenin, a plaque specially bound with Era has been isolated from 4×10⁴ plaques. It is confirmed that an Era-binding protein gene exists in E.coli genomic DNA. Compared with the data in GenBank, the 5′ end 800 bp of inserted DNA is located in the section 267 of E.coli genomic DNA.]]> CHEN Nanchun,CHEN Sumin,GAO Lei,HAO Xiaomeng,GAO Hui,JIN Jing and MA Xiaofeng 106true PU Xiaoping,WANG Jiazheng,ZHANG Jie,GU Xiaosong and FAN Ming 105true 2O₂ or guanidine and hydrolyzed by proteases suggested that these two activities of dismuting O^·₂ and cleaving supercoiled-DNA involved in different sites in SODs.]]> LING Jun,RUAN Kangcheng and LIU Wangyi 104true Agkistrodon acutus. It was shown to be a single band both in SDS-PAGE and IEF. Its molecular weight is about 38 ku and isoelectric point is near 4.0. Also, its 15 amino acid sequence of N-terminal was obtained. It is VIGGVECDINEHRFL. It has high homology with thrombin-like enzymes from other snake venoms.]]> DU Xiaoyan,ZHU Hong,JIANG Kexian,YU Henian and ZHOU Yuancong 103true LONG Jian-yin,LIU Jian-qiang,XUE Yan-ning and WANG Hui-xin 102true LONG Jian-yin,XUE Yan-ning,SUN Lei and WANG Hui-xin 101true HU Jun,ZHANG Zhi-hong,OUYANG Zhen-qian,CHEN Shen-fu and LI Min-qian 100true WU Yi-pin,MA Xiao-jun,HUANG Jia-xue,GAO Hong,ZHANG Xi-yuan and Z.XUE 99true 1-acid glycoprotein(α₁-AGP) by acid hydrolysis, were derivatized with 2-aminoacridone(AMAC), and then separated by capillary electrophoresis, separately. The quantities of sialic acids in the first two glycoproteins were consistent with the previous results while inconsistency was found in the bovine α₁-AGP case. It was found that in bovine α₁-AGP N-glycoloyneuraminic acid (Neu5Gc) was present in addition to Neu5Ac with a quantity comparable to that of Neu5Ac. Based on these data, the AMAC-derivatized sialic acids were unstable and could undergo a decarboxylation reaction at room temperature under light, but they were stable at －20℃in the dark for at least 12 h.]]> CHE Fa-Yun,SHAO Xiao-Xia,WANG Ke-Yi and XIA Qi-Chang 98true YU Ying,XIE Yi,CAO Li,ZHANG Bi-Cheng,ZHOU Ming,ZHAN Feng-Huang and LI Gui-Yuan 97true P＜0.01), and the BRD7 gene might have two modes of translation and the terminational SNP at 450 nt in BRD7 gene results in the second alternatively truncated isoform.]]> YU Ying,ZHU SHI-Guo,XIANG Juan-Juan,LI Zhong-Hua,ZHANG Bi-Cheng,CAO Li and LI Gui-Yuan 96true LI Jiang,TAN Chen,XIANG Qiu,ZHANG Xiao-Mei,MA Jian,LI Wei-Fang,WANG Jie-Ru,YANG Jian-Bo,LIANG Song-Ping and LI Gui-Yuan 95true E.coli BL21. Induced by 0.5 mmol/L IPTG, The band corresponding to fusion protein were observed in SDS-PAGE as expected. Together with bioinformatic analysis of LRRC4 protein, these results establish the basis for functional study of LRRC4.]]> WANG Jie-Ru,DONG Li,JIANG Ming,TAN Chen,LI Xiao-Ling,XIANG Juan-Juan,FAN Song-Qing,PENG Cong,TANG Ke and LI Gui-Yuan 94true Cinnamomum camphora). Recently, a small RIP named as cinphorin was isolated from the seed of camphor tree. The reduced cinphorin exhibited the RNA N-glycosidase activity and inhibitory activity to protein synthesis in vitro like the reduced cinnamomin did. Cinphorin B-chain exhibited the same N-terminal 10 amino acid sequence and molecular mass as the B-chain of cinnamomin. Its A-chain exhibited the same 10 N-terminal amino acid sequence and owned a C-terminal cysteine residue linked to the B-chain, but the molecular mass of cinphorin A-chain was only half of cinnamomin A-chain. RT-PCR and Northern blotting revealed that there was no corresponding mRNA of cinphorin. Cinphorin is probably produced from cinnamomin by protein splicing.]]> HOU Fa-Jian,LIU Ren-Shui and LIU Wang-Yi 93true TAN Chen,LI Jiang,WANG Jie-Ru,Peng Cong,XIE Yi,CAO Li,LI Xiao-Ling and LI Gui-Yuan 92true WANG Nan,LIU Zhi-Hua,DING Fang,WANG Xiu-Qin,ZHOU Chuan-Nong,WANG Ming-Rong and WU Min 91true Chen De-Liang,Li Hui,Han Bao-Shan,Xu Bing and Hu Kun-Sheng 90true 1-ATPase (the mutant α-C193S, γ-S107C, a ten histidine (His) tag inserted immediately downstream of the β initiation codon, α₃β₃γ subcomplex) of thermophilic Bacillus PS3 was purified from E.coli. JM103 Δ(uncB-uncD)，in which the majority of F₁-ATPase genes have been eliminated. It was found that the enzyme hydrolyzed ATP more efficiently than previous papers. During the F₁-ATPase hydrolyzing ATP, the rotary rate of the fluorescent actin filament attached to γ subunit of F₁-ATPase was about one times faster than that of previous papers in the similar conditions.]]> WANG Xing-Sheng,CUI Yuan-Bo,ZHANG Ying-Hao,YUE Jia-Chang and JIANG Pei-Dong 89true TIAN Rui,QU Mei-Hua,LIU Ying and HE Rong-Qiao 88true P＜0.05）between the G867A mutation and chicken skin color , between the C1292T mutation and chicken live body shank color and between the C1377G mutation and chicken meat color. The results of the study indicated that MC1R gene is the candidate gene that regulates melanin traits in chicken.]]> YANG Yong-Sheng,DENG Xue-Mei,LI Ning,FU Yan,ZHU Mu-Yuan and WU Chang-Xin 87true LIU Qin,ZHAO Xin-Gang,ZHANG Ying,SHEN Yi,LIU Yi-Xun and YAN Xi-Yun 86true HAN Wei,ZHANG Pan-He,CAO Wu-Chun,YANG Dong-Ling,SHIGEHARU TAIRA,YOSHIO OKAMOTO,JUN-ICHIRO ARAI and YAN Xi-Yun 85true TAN Zhi-Ping,LIU Yu,CAI Fang,PAN Qian,HE Li-Qiang,HUANG Liang-Qun,DAI He-Ping,XIA Kun,XIA Jia-Hui and ZHANG Zhuo-Hua 84true LI Wei,WAN You,HAN Ji-Sheng,DING Jiu-Ping and XU Tao 83true 2^{) locate in the center of tumor islands in wildtype mice, whereas sparse and enlarged vessels (47/mm2) are evident in tumor islands in AQP1 knockout mice coincident with larger necrotic area in outer layer of the islands. These results provide definitive evidence that lacking of water channel AQP1 results in defective tumor angiogenesis and retarded tumor growth that may be involved in insufficient blood supply and/or abnormal transendothelial fluid transport. The underlying molecular and cellular mechanisms are worth further investigation.]]>} FENG Xue-Chao,Gao Hong-Wen,HE Cheng-Yan and MA Tong-Hui 82true ZHANG Zhi-Xiang and LI Mian-Qian 81true Smu.260 encodes a putative protein of 200 residues in Streptococcus mutans, a primary pathogen for human dental caries. Smu.260 was cloned into expression vector pET28a and expressed in good amount from the E.coli strain BL21 (DE3). Smu.260 protein was purified to homogeneity in a two-step procedure of Ni²⁺ chelating and size exclusion chromatography. The purified protein exists in two forms, a dimer form about 46 ku with yellow color and a tetramer form without apparent color. Crystals were obtained from the dimer protein by hanging-drop vapor-diffusion method. The crystals diffracted to about 2.3 Amstrong resolution and belong to orthorhombic space group P2₁2₁2₁ with cell dimensions of a=89.88 Amstrong, b=90.91 Amstrong, c=105.17 Amstrong. The asymmetric unit is expected to contain two dimers with solvent content of 53%.]]> ZHANG Xiao-Yan,MI Wei,ZHOU Yan-Feng,LIU Xiang-Yu,LI Lan-Fen,LIANG Yu-He,WEI Shi-Cheng and SU Xiao-Dong 80true LIU Yu,TAN Zhi-Ping,PAN Qian,HE Li-Qiang,CAI Fang,WU Ling-Qian,LIANG De-Sheng,XIA Kun,XIA Jia-Hui and ZHANG Zhuo-Hua 79true QIU Wei,ZHAO Liang,Bai Su-Xia,SANG QING-XIANG AMY and Wang Yan-Ling 78true in vitro and in vivo. Furthermore, It was confirmed by atom force microscopy (AFM) that TSH1 binds to GCC element (core sequence GCCGCC) not DRE element (core sequence GCCGAC), and the single molecular force between TSH1 and GCC element was quantitatively measured. The molecular force of TSH1 with GCC element is (83.9 ± 2.2) pN. This interaction can be observably blocked by the dissociate TSH1 protein in the solution. These above results proved that TSH1 recognizes and binds to GCC with specifically interaction, which also demonstrate the high sensitivity of the AFM measurements for the single base substitution of the GCC core sequence greatly reduced the binding affinity. Comparing these methods for identification the binding of transcription factor with its DNA element, it was considered that AFM is expected to be a sensitive and reliable method that offers valuable information for the characterization of transcription factors.]]> 2006/7/17 0:00:00 CHEN Feng,YU Jun-Ping,Fang Xiao-Hong and Zhang Gui-You 77true 2006/7/17 0:00:00 Zhang De-Xin,ZHAO Peng-Tao,ZHAO Qing-Chuan,LUO Xiao-Xing,NIE Yong-Zhan,SU Ying-Jun,YANG Jing-Hua,R.RABINOVICI and FAN Dai-Ming 76true in vitro model to investigate the involvement of MMP-26 in trophoblast cell invasion. Expressing pCR3.1 plasmid containing MMP-26 cDNA was transfected into JEG-3 cells, and a stable cell line with over-expressed MMP-26 was named as JEG-3/MMP-26. Transwell insert invasion assay showed that cell invasiveness in JEG-3/MMP-26 cells was significantly promoted. Data of RT-PCR and gelatin zymography demonstrated that the mRNA expression and zymogen secretion of MMP-9 were increased in these cells. Double immunofluorescent staining revealed co-localization of MMP-26 and MMP-9 proteins in JEG-3/MMP-26 cells. The data indicated that MMP-26 was involved in invasion-promoting regulation of human trophoblast cells, and it may function, at least in part, through coordination with other MMPs such as MMP-9.]]> 2006/6/14 0:00:00 Wu Xiao-qiu,Sang Amy Qing-xiang,Fu Ya-yuan,Li Dian-jun,Li Yu-xia and Wang Yan-ling 75true 2006/10/18 0:00:00 Zhou Yue-Ming,Wang Wei-Ping,LIANG Hua-Zheng,ZHANG Xie,Chen Huan-Wen and CHEN Lan-Hui 74true + channel has been considered as the cardiac sodium channel. Na⁺ currents with tetrodotoxin resistance (TTX-R) and Nav1.5/SCN5A mRNA have been observed in neurons, but the cDNA encoding the TTX-R Nav1.5 Na⁺ channels in human central nervous system (CNS) has not been identified. Nav1.5/SCN5A cDNA was first cloned from human brain cortex by using RT-PCR method. Two variants of Nav1.5/SCN5A were found and tentatively named hB1 and hB2.Full sequence of cDNA encoding the α-subunit of TTX-R Nav1.5 Na⁺ channel in human brain cortex was 6 201 nucleotides long and was designated hB1. The longest open reading frame of hB1 (accession number EF629346 ) encodes 2 016 amino acid residues. Sequence analysis has indicated that hB1 is highly homologus with human cardiac Nav1.5/SCN5A with >98% amino acid identity. There are 28 different amino acids between them, with 7 of which locating in the region encoded by exon6A or exon6. Alternative splicing of exon18 was not found in the gene cloning of human brain Nav1.5/SCN5A, which was different from human heart Nav1.5/SCN5A, but a novel alternative splicing lacking exon24 was first found. The two variants were detected in similar ratio in brain, but they were proved to relate to age development in heart tissue. The exon24 of human Nav1.5/SCN5A has 54 nucleotides, encoding 30 amino acid residues, and are located in human chromosome 3P21. This alternative splicing was also found in other tissues other than heart and brain. The expression pattern of the two variants in different tissues was different when detected by competitive PCR method and it was also changing with age development. Furthermore,Nav1.5/SCN5A mRNA was detected in 16 different tissue types of Wistar rats (P80) by reverse polymerase chain reaction (RT-PCR). These results suggest that Nav1.5 Na⁺ channels in human brain are encoded by new variants of Nav1.5/SCN5A and its mRAN is more widely expressed than previously thought. The study is useful for making further investigation in the functional analysis of Nav1.5 Na⁺ channels in different tissues.]]> 2007/9/17 0:00:00 OU Shao-Wu,ZONG Zhi-Hong,WANG Jun and WANG Yun-Jie 73true 2007/8/13 0:00:00 REN Cheng-Tao,OU Shao-Wu,WANG Yun-Jie,LIN Yi and ZONG Zhi-Hong 72true Cucumber mosaic virus (CMV) CB7 strain, causing necrosis on Nicotiana glutinosa, were obtained by RT-PCR, using viral genomic RNAs as templates. cDNAs of CMV-CB7 genomic RNAs were cloned and sequenced and results indicated that RNA1, 2 and 3 was 3 356 nt, 3 045 nt and 2 218 nt, respectively (accordingly Accession Number EF216866, DQ785470 and EF216867). Infectious RNA transcripts from cDNA clones of CMV-CB7 were inoculated onto N. glutinosa and the seedlings of host plants displayed necrosis symptom, whist that of CMV-Fny induced typical mosaic symptoms. Through pseudorecombination between CMV-CB7 and CMV-Fny genomic RNAs, the genetic determinant of necrosis phenotype was mapped to RNA2. Chimeric infectious clones consisting of partial sequences of RNA2 derived from CMV-CB7 and CMV-Fny, respectively, were obtained by Overlapping PCR. Pathogenic analysis with those chimeric RNA2 revealed that 2b gene or 3′UTR of CMV-CB7 RNA2 was responsible for the necrotic pathotype. Northern blotting analysis reflected that both necrotic and non-necrotic viruses accumulated to similar levels of genomic RNAs in host plants. Therefore, necrotic phenotype induced on N. glutinosa was not related to the level of accumulation of CMV genomic RNAs.]]> 2007/7/2 0:00:00 LIAO Qian-Sheng,DU Zhi-You,ZHANG Hua-Rong,ZHU Li-Ping,WU Peng and CHEN Ji-Shuang 71true in vitro. Taken together, hMSCs may suppress tumor growth via Wnt/β-catenin pathway, in which Dkk-1 released from the hMSCs is responsible for the depression.]]> 2007/5/30 0:00:00 QIAO Ling,XU Zhi-Li,TAN Xu,YE Li-Hong and ZHANG Xiao-Dong 70true 2007/5/22 0:00:00 王启宇,王惟,乔晓杭 and 唐捷 69true 2007/4/17 0:00:00 Zhang Ling and Huang xinhan 68true + channel. Compared to its mRNA transcription in the heart, the Nav1.5/SCN5A gene was also expressed in high levels in the brain, especially limbic regions of the brain. Exon6A of Nav1.5/SCN5A gene was first found in cloning of Nav1.5 channel in human neuroblastoma cells. It was found that it is exon6A that encodes Nav1.5 channels in human and rat brain while it is exon6 that encodes Nav1.5 channels in other tissues in the cloning of Nav1.5 channel in these tissues, Both exon6A and exon6 have 92 base pairs, which encodes 30 amino acid residues, and are located in human chromosome 3. But these two resulting products differ by 7 amino acid residues. There is only one different base pair between the enon6As encoding Nav1.5 channels in human and rat brain, but their encoding amino acid residues are identical. The expressions of exon6A in different part of the brain are different and the similar result was found in the expressions of exon6 among different tissues of rat when detected by using RT-PCR method. The study is useful for making further investigation in the functional analysis of Nav1.5 channels in different tissues.]]> 2007/2/28 0:00:00 WANG Jun,ZONG Zhi-Hong,OU Shao-Wu,WANG Yun-Jie,REN Cheng-Tao,LIN Yi and MENG Xiao-Na 67true P＜0.01). Introducing the caveolin-1 gene into Hs578T/Dox cells enhanced their migration and invasive capability, as revealed by an in vitro chamber invasion assay(P＜0.01). Apoptosis index of Hs578T/Dox+cav-1 group resulting from loss of cell-matrix interactions decreased comparing Hs578T/Dox+vector group(P＜0.01). Caveolin-1 inhibited anoikis of Hs578T/Dox cells and that allowed survival of cancer cells during systemic circulation, thereby facilitating secondary tumor formation in distant organs. The efficacy in vivo of caveolin-1 was tested using cells xenografted into nude mice. Each mouse in both fifteen-mice groups were hypodermically injected with equivalent doses of 5×10⁵ cells from Hs578T/Dox+cav-1 or Hs578T/Dox+vector. Tumors in Hs578T/Dox+cav-1 group were all formed after injection followed for 4 weeks, while no one in Hs578T/Dox+vector group. Lung metastasis was found in one mouse injected Hs578T/Dox+cav-1 cells. In conclusion, up-regulated caveolin-1 level in Hs578T doxorubicin-resistanted cells markedly elevates their capacity to tumor invasion and metastasis.]]> 2007/1/15 0:00:00 ZHENG Ya-Min,LI Fei,QI Bao-Ju,WU Ping,SUN Hai-Chen,LIU Shuang and CHEN Jian-Wen 66true P < 0.05) and normal skin (P < 0.05), however, no significant difference of Smad2 , 3 and the phosphorylation of Smad2,3. But the mechanism of reduced expression of Smad7 is unclear. Sp1 binding sites were found in Smad7 promotor by bioinformatics system analysis, then, the expression of TIEG1 mRNA and proteins in keloids, in normal skin and in normal scars tissues and fibroblasts were investigated. Dermal fibroblasts were obtained from biopsies of keloids, normal scars and normal skin. Fibroblasts were cultured in vitro. The expression of TIEG1 mRNA was analysed by RT-PCR and protein expression was determined by Western blot analysis. The results demonstrated increased mRNA and protein expressions of TIEG1 in keloid tissue and fibroblasts as compared to normal scar tissues and fibroblasts (P < 0.05) and normal skin tissues and fibroblasts (P < 0.05). It suggested that TIEG1 might play a significant role to the decreased expression of the inhibitory Smad7 in keloid. Further investigation is necessary to illuminate the mechanism of TIEG1 regulating Smad7.]]> 2007/10/22 0:00:00 TANG Bing,ZHU Jia-Yuan,ZHU Bin,LIU Yang,LI Xin-Qiang and BI Liang-Kuan 65true 2008/8/1 0:00:00 CHANG Wei-Shan,ZHAI Jing,SONG Wen-Gang and LIU Yong-Qing 64true Drosophila brain gene expression pattern and its database. Large genome-wide screen for specific expression pattern in adult Drosophila brains by enhancer trap method provides fly tools for further understanding neural basis of learning and memory. Enhancer trap method was utilized to generate Drosophila P[Gal4] mutant library. Each mutant in library was crossed with GFP reporter flies (UAS-eGFP); heads of the adult off-springs were dissected to obtain the whole brain which were examined under the epifluorescence microscope and expression pattern of each mutant were further analyzed. JavaScript was utilized to establish an expression pattern library. A total of 2 677 mutant lines of Drosophila were obtained by P-element induced mutagenesis and subjected to expression pattern analysis. Most of mutated genes express in adult fly. 368 mutated genes specific express in the mushroom body, which was reported to involve in Drosophila olfactory learning and memory, and some others specific express in other neuron circuitries. Drosophila mutant libraries and genome-wide expression pattern database was built by enhancer trap method, which as a tool will be utilized to understand the function of genes and particular neural circuitries in Drosophila in the future.]]> 2008/6/16 0:00:00 LIU Xiao-Jun,YUAN Xiao-Jing,SUN Kan,SHUAI Yi-Chun,SONG Qing-Xuan,WANG Lei,SHAO Li-Sha,ZHAO Xin-Yu,LU Yi-Sheng,LU Yu-Bing,WANG Lian-Zhang and ZHONG Yi 63true INK4a, PTEN/p27^Kip1 and p53/p21^Waf1 protein levels were detected by Western blot. The result showed that ING1a, ING1b, ING1c, and 1a△C except for 1b△C induced p16^INK4a protein expression, in which ING1c had the most powerful effect. Luciferase assay identified that overexpression of pcDNA3.1(+)-1a△C facilitated p16^INK4a transcription through enhancing p16^INK4a promoter activity, while pcDNA3.1(+)-1b△C repressed the p16^INK4a promoter activity . In a word, it was found for the first time that except for the p53/p21^Waf1 pathway, three splicing isoforms of ING1 family could also inhibit HeLa cells proliferation though upregulation of p16^INK4a and PTEN, and the PHD domain deletion of ING1a enhanced p16^INK4a transcription. These findings provide new clews to further study on the mechanisms of ING1 family suppressing cancer cells growth.]]> 2008/5/23 0:00:00 LI Na,ZHANG Ying-Tao,XUE Li-Xiang and TONG Tan-Jun 62true 18F-FDG) positron emission tomography (PET) was used to observe brain glucose metabolic changes in those patients. Five patients with chronic pain due to left-BPA, whose pain reduced more than 75% after dorsal root entry zoneotomy (DREZotomy) were selected. The visual analog scale (VAS) , Hamilton depression scale, Hamilton anxiety scale and ¹⁸F-FDG PET of brain were recorded before and 14 days after DREZotomy respectively. Statistical parametric mapping 2 (SPM2) was applied for data analysis. Comparing with PET during pain before DREZotomy, PET after DREZotomy showed significant glucose metabolism decreases in bilateral caudate, orbitofrontal cortex (OFC) (BA11), contralateral subgenual cingulate (BA25) and ipsilateral dorsolateral prefrontal cortex (DLPFC) (BA46/47), and significant glucose metabolism increases in contralateral thalamus, pulvinar and ipsilateral parietal lobe (BA7). The results suggested that the brain areas involved in emotion, attention and internal modulation of pain play an important role in the modulation of chronic pain due to BPA.]]> 2008/4/17 0:00:00 CHEN Fu-Yong,TAO Wei,CHENG Xin,ZHOU Ke,SHAN Bao-Ci,YUAN Xiu-Li,HU Yong-Sheng,ZHANG Xiao-Hua and LI Yong-Jie 61true in vivo. According to recent literatures, endogenous formaldehyde production is related to several metabolic pathways such as amine oxidation (catalyzed by semicarbazide-sensitive amine oxidase, SSAO), methylation and demethylation. Under oxidation stress and energy metabolic imbalance, formaldehyde markedly increases in human circulation. Here, the authors found that formaldehyde is released from the reaction of malondialdehyde with a protein (BSA) in which protein side chains such as amino groups are chemically modified. Moreover, formaldehyde is also produced from the sphingomyelin solution in the presence of hydrogen peroxide as the myelin peroxidation occurs. Formaldehyde at low concentration induces neuronal Tau aggregation, resulting in formation of globular like aggregates which are toxic to SH-SY5Y cells, HEK-293 cells and hippocampus neurons in the primary culture. According to Chen et al. (2006), endogenous aldehydes are related to beta-amyloid misfolding, oligomerization and fibrillogenesis. Furthermore, formaldehyde is able to react with some neurotransmitters and thus impairs their structures and functions. Under physiological conditions, the human blood formaldehyde is dynamically kept approximately (0.087±0.004) mmol/L. Notably, this concentration is close to the half-lethal dose of formaldehyde (0.10～0.12 mmol/L) to neural cells in the in vitro cell culture such as SH-SY5Y cells. Furthermore, cell growth can be partially affected and inhibited in the presence of formaldehyde at (0.087± 0.004) mmol/L during the in vitro culture. This suggests that human body needs a strong degradation system to remove endogenous formaldehyde. As shown in clinical trials, the formaldehyde level in urine of Alzheimer's patients was markedly higher than the control subjects. The urine formaldehyde level was shown to be related to the cognitive impairment. Therefore, the level of blood (brain) formaldehyde is supposed to be changeable and increased under aging, leading to a higher risk chance to impair human brain, especially under stressing. The formaldehyde chronic damage to neural cells (grey mater) and neural fibers (white mater) may be one of the most important pathological mechanisms for sporadic neurodegeneration for instance Alzheimer's disease, because hypofunction in scavengering endogenous formaldehyde occurs as aging.]]> 2008/4/17 0:00:00 LI Fang-Xu,LU Jing,XU Ya-Jie,TONG Zhi-Qian,NIE Chun-Lai and HE Rong-Qiao 60true in vivo. Importantly, co-expression of NOK and JAK2 produced a synergistic effect on NOK-mediated STAT3 activation, while inactivating the kinase domain of JAK2 completely prevented this synergistic effect. Overall, the results indicated that NOK might complex with both STAT3 and JAK2 and activate STAT3 signaling by a JAK2-dependent mechanism.]]> 2008/2/18 0:00:00 LI Ying-Hua,RONG Yu,CHANG Zhi-Jie and LIU Li 59true 2007/12/19 0:00:00 LI Ying-Hua,CHANG Zhi-Jie and LIU Li 58true 2009/5/25 0:00:00 WU Zhi-Yin,HE Cheng-Qiang,LIU Ying-Ying,FENG Qian,TENG Jun-Lin and CHEN Jian-Guo 57true Tombusvirus P19 also serves as a suppressor of RNA interference (RNAi) by sequestering intracellular small RNAs such as the small interfering RNAs (siRNAs) and microRNAs (miRNAs). However, the effect of P19 on mammalian cells has not been evaluated before. A human embryonic kidney 293 cell line that stably expressed p19 (HEK293-p19) was generated. Flow cytometric analysis revealed that over-expression of P19 caused a significant accumulation of G2/M phase cells. Cell proliferation assays demonstrated a reduced DNA replication and cell growth in HEK293-p19 cells. Moreover, p19 altered the expression profiles of a number of cell cycle regulators in HEK293 cells, such as upregulation of cyclin A1, CDK2, CDK4, CDK6, p18, cyclin D2, p19INK4d and E2F1, and downregulation of p15, cyclin A2, cyclin B1 and cyclin E1. Thus, the data strongly indicate that p19 might influence multiple G2/M regulators to cause G2/M arrest.]]> 2009/4/2 0:00:00 LIU Li,LI Jian,XU Yu-Ping,QIAO Wen-Tao,CHEN Qi-Min and GENG Yun-Qi 56true sec-10 (one component of the exocyst complex) knockout mutant of C. elegans was obtained for the first time. The drug sensitive assays revealed clearly that the sec-10 gene affected the neural signal transmission, however, the electrophysiological assay showed the function of the ionotropic receptors in the neuromuscular junctions (NMJs) were unaltered compared with the wild type (WT). Thus it was assumed that the sec-10 gene might not influence the known ionotropic receptors in the NMJs, but some other pathways instead.]]> 2009/4/17 0:00:00 ZHANG Lei,LI Jiang-Li,GAO Shang-Bang,WU Zheng-Xing,ZHANG Rong-Ying and XU Tao 55true 2009/9/1 0:00:00 HUANG Bo,LI Wen-Ting,LI Wen,XIA Xue-Feng and SUN Zhi-Rong 54true 2009/9/1 0:00:00 ZHOU Hai-Jun,HEI Zhen-Yu,SHI Jiong,GUO Kun,SUN Bing-Sheng,WU Jin-Cai,ZHAO Yue,FU Li-Yun,DAI Chun,GAO Dong-Mei,SUN Rui-Xia,ZHAO Yan,CHEN Jie,WANG Lu,QIN Lun-Xiu and LIU Yin-Kun 53true Danio rerio) is an ideal vertebrate model organism for the study of developmental processes, especially for that of cardiovascular formation. 26 transgenic fish lines with blood vessel-specific EGFP expression were identified via a large scale enhancer trap screen mediated by Tol2 transposon in zebrafish. The EGFP expression in some of these lines shows different and unique patterns in different part of blood vessels. The genomic sequences flanking the Tol2 insertion sites have been successfully cloned from 22 lines via linker-mediated PCR, among which 17 sequences could be mapped to a unique location within current zebrafish genome assembly. Expression of 8 flanking genes from 9 transgenic lines was confirmed to recapitulate the expression of EGFP reporter gene in their corresponding lines via RNA whole mount in situ hybridization (ISH). Three of these genes, hhex, ets1a and dusp5 are known to be important for vasculargenesis. Since hhex and ets1a are also expressed in hematopoietic precursors, these transgenic zebrafish should be very useful for the study of both hematopoiesis and vasculargenesis. The rest of these genes, namely zvsg1, micall2a, arl8b (1 of 2), zgc:73355 and hecw2 (1 of 2), are either novel or functionally unknown in zebrafish. Further investigation of these fish lines and corresponding genes will give important insights of blood vessel developmental mechanisms including hemangioblast formation and differentiation, as well as genes and enhancer elements important for cardiovascular system. In addition, these transgenic fish lines could also make invaluable contributions to small molecule screen for drug discovery.]]> 2010/7/21 0:00:00 XUE Yu-Lin,XIAO An,WEN Lu,JIA Yue,GAO Yue,ZHU Zuo-Yan,LIN Shuo and ZHANG Bo 52true hiCD49f⁺ and CD24^medCD49f⁺, and their percentages in total mammary anlagen cells were 16% and 47%, respectively. In the following monolayer culture and in vivo transplantation tests, it was found that the CD24^medCD49f⁺ cells could attach plate and regenerate mammary ductal units; correspondingly, CD24^hiCD49f⁺ cells did not possess of these capabilities. These results indicate that the two mammary anlagen cell populations represent different cell types, and CD24^medCD49f⁺ population may contain the self-renewal mammary anlagen stem/progenitor cells. Afterwards, the differences in the expressions of 19 mammary-related CAM transcripts in the two cell populations were validated by quantitative real-time PCR analysis. The data show that the CAM gene expressions of mammary repopulating-CD24^medCD49f^{+ cells are dramatically different from that of CD24hiCD49f+ cell population. The expressions of several adult stem cell markers in CD24medCD49f+ cell population are remarkable higher than those in CD24hiCD49f+ cell population.]]>} 2010/4/28 0:00:00 SONG Jia-Zhe,XUE Kai,LI Song and LI Ning 51true et al (University of California, Santa Barbara) to explain the mechanism of metabolic dysfunction and cell death during protein conformational diseases, the "pore-like" aggregates of misfolded neural Tau have not been observed. Revulsants involved in the formation of "pore-like" aggregates have not been found yet. According to the hypothesis "chronic impairment resulted from abnormally-increased endogenous formaldehyde is one of the important risk factors related to sporadic senile dementia", formaldehyde has been utilized to incubate with Tau protein resulting in amyloid-like deposits with marked cytotoxicity. Under the experimental conditions, 0.5% formaldehyde-treated Tau could form "pore-like" aggregates. These results may deliver a novel approach to study the mechanism of cellular metabolic disturbance, even cell death, which is induced by formaldehyde-treated neural Tau.]]> 2010/10/26 0:00:00 Sajjad Haider Naqvi,WANG Wei-Shan,MIAO Jun-Ye and HE Rong-Qiao 50true 2+ image studies were carried out. It was found that inositol 1, 4, 5-trisphosphate receptors (IP₃Rs) and endoplasmic reticulum protein 44 (ERp44) co-localized on the nuclear envelope and endoplasmic reticulum (ER). Extracellular ATP induced nuclear calcium transient via IP₃Rs and subsequently increased the cAMP response element binding protein (CREB) phosphorylation and the expression of c-Myc. However, all these were inhibited by 2-aminoethoxydiphenyl borate (2-APB), an IP₃Rs inhibitor, and by over-expression of ERp44. These results suggest that ERp44 inhibits gene transcription via IP₃Rs in HeLa cells.]]> 2011/6/17 0:00:00 PAN Cong-Yan,ZHOU Rong-Bin,CHEN Zheng,CHEN Ying-Xiao,WU Yan-Yun,MIAO Lin,YIN Wen-Xuan and JI Guang-Ju 49true IC₅₀ was from 1∶256 to 1∶64. When detected with 8G10D7, all 4 viruses showed 70 ku and 43 ku protein band, which confirms that the binding site of the scFv antibodies were located at the HA1 domain. The nucleuses of MDCK cells infected by 4 viruses were colored purple, and red around the nucleus. 8G10D7 showed HI activity to the 4 viruses, the HI has a positive correlation with neutralization concentration IC₅₀, which also further confirms that the binding site of the scFv antibodies were located at the HA1 domain. When the mAb 8G10D7 was used for the study of prophylaxis and therapeutic effect on influenza A viruses infection in an embryonated chicken eggs model. It had a complete 100% protection effect on the H5N1 viruses in avian host in the prophylactic and therapeutic groups. The 100% preventive protection effect could be reached when challenged with H5N1 avian influenza A viruse in human host in the prophylactic groups, and there is also a 87.5% protection effect with H5N1 viruses in human host in the therapeutic groups. Thereby, the study suggests that the mAb 8G10D7 could be used in therapies to counter the H5N1 influenza A virus, and the epitope could be the key point for the design and implementation of vaccines.]]> 2011/5/20 0:00:00 ZHANG Xiao,ZENG Xiao-Yan,LIU Zhe,JIN Qiu,XU Yan,FENG Zhen-Qing and JIAO Yong-Jun 48true Eisenia fetida proteaseⅠ(EfP-Ⅰ), showing that the interaction between this protease and its substrates underwent an "induced fit" followed by "lock and key" model. It needs further investigating whether this model is suitable for other enzymes. Here, the reactions of substrate-induced Eisenia fetida proteaseⅡ(EfP-Ⅱ), subtilisin (Sub) and lactate dehydrogenase (LDH) with their substrates were shown. EfP-Ⅱ and Sub could not recognize chromozym U (CU) (P < 0.05) after incubated with chromozym TH (CTH) although the two proteases are natively able to react with both CTH and CU. The reaction followed an "induced fit-lock and key" pattern. In contrast, the two proteases were still able to react with CTH even though they have been incubated with CU. But neither earthworm protease nor subtilisin could recognize CU after CU and CTH treatment in turn, still suggesting that the reactions followed an "induced fit and then lock and key" procedure. Furthermore, the activity of LDH with lactate significantly decreased (P < 0.05) after the enzyme had been incubated with pyruvate. The activity on the conversion of pyruvate into lactate was not significantly affected by a prior incubation with lactate. This suggests that the pyruvate-induced complementary conformation of LDH is more stable than lactate-induced conformation.]]> 2011/4/13 0:00:00 ZHANG Zi-Jian,PAN Rong,ZHOU Yuan and HE Rong-Qiao 47true + CPCs in tissues and living cells, Tbx18 genetic fate-mapping model, Tbx18-Cre/Rosa26R-EYFP and Tbx18-Cre/Rosa26R-LacZ double-heterozygous mice, were founded by Cre-LoxP system. These double-heterozygous mice were useful in monitoring the fate of Tbx18⁺ cells by Cre expression, and tracing the lineage of the cells in embryos and adult mice. YFP⁺ cells can be easily isolated from heart of Tbx18-Cre/Rosa26R-EYFP double-heterozygous mice by fluorescence activated cell sorter(FACS) and be monitored under an inverted confocal microscope. X-gal staining of genetic fate-mapping model revealed that atria, ventricular septum, ventricular wall, coronary vascular and atrioventricular valves can be generated from Tbx18 lineages. Here it was showed that Tbx18⁺ CPCs can differentiate into anticardiac troponin T(cTNT ) positive cardiomyocytes and anti-smooth muscle myosin heavy chain11 positive smooth muscle cells in vivo by immunofluorescence. The heart is a complex organ composed of a diverse set of muscle and non-muscle cells. It was speculated that Tbx18 may play an important role in regulating the signal pathway of progenitor cells differentiating into myogenic cell lineages. These results identify Tbx18 as a marker of cardiac progenitors cells by Tbx18 genetic fate mapping mice, and lay the foundation for researching the differentiation potential of cardiac progenitors into cardiomyocyte lineages in the field of cardiac regeneration and repair.]]> 2011/1/13 0:00:00 DU Jian-Lin,ZHANG Jin,PU Di,GAO Er-Zhi,YANG Jing-Tao,GAO Ling-Zhi,XIA Shuang,DENG Song-Bai and SHE Qiang 46true in vitro. These findings reveal that hyperphosphorylation declines Tau protein to protect DNA, and may thereafter lead to damage of DNA and even cell death, giving a novel viewpoint to the pathology of Alzheimer's disease.]]> 2011/12/21 0:00:00 LU Jing,MIAO Jun-Ye,PAN Rong and HE Rong-Qiao 45true Pseudomonas aeruginosa secretes the effector protein Tse2, a toxin to other competitive bacteria. It is a newly identified molecular mechanism for P. aeruginosa to win a survival advantage. To avoid being poisoned itself, P. aeruginosa synthesizes the specific immunity protein, Tsi2 to inhibit the toxin. Sequence analysis shows that Tsi2 is a novel antitoxin-like protein which is specific for P. aeruginosa. Using the SAD method, we have successfully resolved the crystal structure of Tsi2 at 1.8Å resolution. Our crystallographic studies reveal that Tsi2 adopts a novel coiled coil conformation which is not found in the antitoxins family previously. Meanwhile, Tsi2 is a well-assembled protein instead of an unfolded protein as an antitoxin in the toxin-free state. Tsi2 functions as a stable dimer and assembles as a unique "clamp" structure through extensive hydrophobic interactions. Two grooves on the dimerization interface combining with one helix of two symmetric molecules respectively imply the potential region interacting with Tse2. This research not only offers comprehensive insights into the molecular essence of Tsi2 as an antitoxin, but also reveals its structural basis of antitoxin activity. Furthermore, the apo Tsi2 structure provides a good framework for further researches on the structure and function of the complex Tse2-Tsi2.]]> 2012/5/18 0:00:00 WANG Wei,DING Jing-Jin and WANG Da-Cheng 44true 2012/5/31 0:00:00 DAN Qiong-Jie,LIU Yi-Tong,WU Jia-Wei and WANG Zhi-Xin 43true P < 0.01) and in atypical hyperplasia (44%) (P < 0.05). Importantly, we found that sTn expression in typeⅠ carcinoma (80%) was obviously higher than that in typeⅡ(45%) (P < 0.05). These results suggested that sTn may be a biomarker for endometrial carcinoma, especially for typeⅠcarcinoma. Moreover, we observed that sTn expression was increased with the decline of tumor histological grade (P < 0.05), indicating that sTn was associated with a favorable prognosis in endometrial carcinoma.]]> 2012/5/18 0:00:00 AN Yun-He,ZHANG Hao-Feng,SUN Min,ZHANG Jun,CHEN Xiu-Qin,CHEN Dong,LU Di,FENG Jing,YANG Dong-Ling,SONG Li-Na and YAN Xi-Yun 42true 2012/4/23 0:00:00 WANG Zhi-Jie,GAO Xin,LI Bing and WU Jian-Young 41true 2012/9/14 0:00:00 BI Liang-Kuan,LIN Tian-Xin,XU Ke-Wei,HAN Jin-Li,HUANG Hai,ZHANG Cai-Xia,DONG Wen,LIU Hao and HUANG Jian 40true in vitro and in vivo. Intraperitoneal injection of D-ribose into mice significantly increases their glycated serum protein and blood AGEs though the concentration of D-glucose became slightly decreased, suggesting that D-ribose is much easier to produce AGEs than D-glucose in vivo. Here, using 4-(3-Methyl-5-oxo-2-pyrazolin-1-yl) benzoic acid (MOPBA) coupled with HPLC, we determined the concentration of uric D-ribose of type 2 diabetic patients (n=30) and the age-matched healthy controls (n=30). The results show that the yield of the derivative of MOPBA-ribose is linearly correlated with the concentration of D-ribose (r²=0.999) with a recovery of 99%. The isolated fractions of D-ribose and D-glucose from urine of type 2 diabetic patients through HPLC were analyzed by mass spectrometry, and the results showed that the fractions contained 569.19 u compound (C₂₇H₂₉N₄O₁₀, D-ribose), and 599.20 u compound (C₂₈H₃₁N₄O₁₁, D-glucose) respectively. The concentration of uric D-ribose of Type 2 diabetics (male (134.28±35.09) μmol/L, female (97.33±23.68) μmol/L) was significantly (P < 0.001) higher than that of the age-matched healthy control (male (35.99±5.64) μmol/L, female (33.72±6.27) μmol/L). Under the experimental conditions, the uric D-glucose level of the patients was also markedly (P < 0.001) higher than the control. Further analyses showed a marked increase in the level of uric D-ribose from either male (P < 0.001) or female (P < 0.001), but a significant difference of the uric levels between male and female could not be observed (P > 0.05). The high levels of uric D-ribose and D-glucose of the patients suggest that type 2 diabetic patients are not only suffered from D-glucose metabolism disorders, but also from D-ribose metabolism disorders.]]> 2013/9/16 0:00:00 SU Tao,XIN Liang,HE Ying-Ge,WEI Yan,SONG Yi-Xiang,LI Wei-Wei,WANG Xue-Mei and HE Rong-Qiao 39true 2013/8/21 0:00:00 WU Kai-Yuan,ZHANG Yu-Ying,SU Wen-Ting and CHEN Chang 38true 2013/7/24 0:00:00 MIAO Jun-Ye,LU Jing,ZHANG ZI-JIAN,TONG Zhi-Qian and HE Rong-Qiao 37true 2013/5/22 0:00:00 SUN Di,WANG Bo and ZHOU Tian-Gang 36true via Lys-29 linkages. RNF148 also ubiquitinated TSPAN15 via generating Lys-63 polymerize ubiquitin molecules. These findings demonstrate for the first time that RNF148 is a RING finger ubiquitin E3 ligase and TSPAN15 is one of its substrates.]]> 2013/4/22 0:00:00 SUO Ta-Lin and TANG Jie 35true 2013/3/22 0:00:00 MENG Xian-Kai,SUN Wen-Jian,XU Xin-Xiu,ZHANG Zi-Cong and HE Ju-Fang 34true 2014/9/22 0:00:00 YE Jing-Ying,XUE Lin-Yan,HUANG Dan,YANG Yue-Jiao and CHEN Yao 33true 2014/6/18 0:00:00 CHEN Ya-Qi,ZHOU Tian-Gang and CHEN Lin 32true 2+ release channels, may act as an important regulator of catecholamine secretion. In the present study, the role of FKBP12.6 in the control of chromaffin cell exocytosis has been investigated using FKBP12.6-null mice. The results showed that FKBP12.6 was expressed in mouse chromaffin cells; deletion of FKBP12.6 did not change the depolarization induced Ca² current and exocytosis. However, deletion of FKBP12.6 resulted in an enhanced caffeine-induced global Ca² transient and larger caffeine-induced exocytosis in chromaffin cells of mice. These results indicate that FKBP12.6 is involved in catecholamine secretion through regulation of Ca² release channel in mouse chromaffin cells.]]> 2014/5/22 0:00:00 YUAN Qi,GUO Ning,GU Lei,WANG Jun,WANG Li,ZHOU Zhuan and JI Guang-Ju 31true e.g., triangles and arrows; digits and letters and geometrical shapes), results showed that the crowding effect was significantly reduced when the target and the flankers were topologically different. Control experiments showed that such a reduction of the crowding effect was not due to differences in subjective similarity, or differences in geometrical features such as area and shape. This finding suggests that topological properties play an important role in perceptual grouping which influences the crowding effect.]]> 2014/2/21 0:00:00 WU Rui-Jie,WANG Bo and ZHUO Yan 30true 2014/2/21 0:00:00 TANG Ke,WEI Fang,BO Hao,HUANG Hong-Bin,ZHANG Wen-Ling,GONG Zhao-Jian,LI Xia-Yu,SONG Ya-Li,LIAO Qian Jin,PENG Shu-Ping,XIANG Juan-Juan,ZHOU Ming,MA Jian,LI Xiao-Ling,XIONG Wei,LI Yong,ZENG Zhao-Yang and LI Gui-Yuan 29true Caenorhabditis elegans, and the piRNA loci share a conserved upstream sequence. New piRNAs can be predicted based on its conserved upstream sequence. C. elegans are synchronized and small RNAs from wild type and prg-1 strains at L4 developmental period are sequenced through illumina technology. Basing on piRNA conserved uptream sequence, we find 967 new piRNAs expressed at L4 period, and the expressions of new piRNAs disappear in prg-1 mutant. Most of new piRNAs are mapped into two piRNA clusters of Chromosome Ⅳ, and have an overwhelming bias for a 5' uracil. Compared to the published co-immunoprecipitation data from adult worms, over 100 new piRNAs can interact with PRG-1. However, some non-21nt long sRNAs, which also share the same loci with piRNAs, are also found in wide type worms. These non-21nt long sRNA may be co-products of piRNA precursors. Together, these results indicate that our sRNA sequencing identified many new piRNAs in C. elegans.]]> 2014/12/19 0:00:00 CUI Dong-Ya,WANG Jia-Jia,SUN Xu-Bin,ZHANG Peng,HE Shun-Min and huangdawei 28true 2+ triggered synaptic vesicle fusion contains synchronous and asynchronous components. At the Calyx of Held synapse, synaptotagmin-2 has been identified as the Ca² sensor protein for synchronous vesicle release while the Ca² sensor for asynchronous vesicle release remains unknown. Sr² is a divalent cation similar in size to Ca² and often substituted for Ca² as a tool in studying asynchronous vesicle release. However, the mechanism of Sr² triggering vesicle release is still under debate. We studied the spontaneous and evoked vesicle release at the wild type and synaptotagmin-2 knock-out calyceal synapses in the existence of extracellular Sr² and found synaptotagmin-2 only mediates the Sr² triggered synchronous vesicle release but not the asynchronous and spontaneous release; the unknown divalent sensor for asynchronous vesicle release has high-affinity to Sr² and mediates the spontaneous vesicle release. Our study provided a new insight into exploring the divalent sensor in triggering asynchronous synaptic vesicle release.]]> 2014/11/21 0:00:00 GUO Jian-Li,TIAN Hao and SUN Jian-Yuan 27true 2015/6/24 0:00:00 SHAN Liang, LIU Tian-E, ZHAO Xu-Dong and MA Yuan-Ye 26true Proteobacteria. Five of them were identified as the human opportunistic pathogen Stenotrophomonas matltophila. Four strains were closely related to Mesorhizobium amorphae. It is interesting to see that one isolate A-3-E^T showed low 16S rRNA gene sequence similarity (< 96.8%) to those of the recognized species. The results of polyphasic taxonomy showed the strain represents a novel species of the new genus, for which the name Paramesorhizobium desertii gen. nov., sp. nov. is proposed. Moreover, it was found that high concentrations of β-lactam antibiotics are not able to inhibit the growth of strain A-3-E^T. The novel bacterium grows well in the media containing 1000 mg/L of cefazolin or 250 mg/L of cefotaxime. The strain also resists 17 of the 28 tested antibiotics. We just presented a case that the Taklimakan Desert is a natural reservoir of novel β-Lactam antibiotic resistant bacteria.]]> 2015/1/19 0:00:00 LUO Xue-Song, PAN Yun-Fan, SHI Jing-Jing, DAI Jun, HOU Sha, FANG Cheng-Xiang and PENG Fang 25true in vitro and xenograft tumor growth in vivo. Also, miR-92b was identified as a secreted miRNA, which can be detected in both cultured medium and the peripheral blood of xenograft mice. Furthermore, we demonstrated that c-MYC, which was also elevated in CRC tissues, promoted the transcription of miR-92b by regulating its promoter activity. Luciferase assay and Western blot analysis revealed that FBXW7 was a novel target of miR-92b and can be negatively regulated by c-MYC. As FBXW7 is a major E3 ligase of c-MYC, our data suggested a potential positive regulatory feedback loop among c-MYC, miR-92b and FBXW7 in CRC. Collectively, we partly provided evidence on how miR-92b is regulated and the potential implications of miR-92b in CRC diagnosis.]]> 2016/7/19 0:00:00 YIN Xiao-Fei, WU Shuai, YANG Yuan-Qin, FANG Xian-Long, XU Hai-Neng, LIU Xin-Yuan, ZHENG Shu and ZHANG Kang-Jian 24true Escherichia coli YchF and YihA to the 30S, 50S and 70S ribosomes in the presence of different nucleotides, and assayed whether these binding preferences were associated with the stimulation of their NTPase activities. Our data show that YchF and YihA display a strong preference for the 70S and 50S, respectively. While the 70S ribosome, but not the 50S or 30S, promotes both the ATPase and GTPase activities of YchF, YihA responds to both the 50S and 70S, with the moderate GTPase stimulation (～8.8 fold) seen in the presence of the 70S ribosome.]]> 2016/6/21 0:00:00 KONG Meng-Yuan, YAN Kai-Ge, MA Cheng-Ying and GAO Ning 23true 2016/10/24 0:00:00 SHENG Chun-Hai, SUN Lu, CHU Xiao-Yu, LI Chang-Fei and MENG Song-Dong 22true P < 0.01) elevated in the chronic cerebral hypoperfusion rats compared with those with SHAM. LeuLeuOMe was used to induce the permeabilization of lysosome membrane in bEnd.3. After LeuLeuOMe tretment, higher intracellular and lower extracellular formaldehyde were measured by microplate reader and high performance liquid chromatography (HPLC) respectively. In other words, lysosome not only accommodates endogenous formaldehyde, but also transports the compound out of cells. Abnormal lysosome function causes dysmetabolism of formaldehyde, which is correlated with age-related cognitive impairment.]]> 2017/6/20 0:00:00 CHEN Xi-Xi, SU Tao, HE Ying-Ge and HE Rong-Qiao 21true 2017/5/22 0:00:00 ZHOU Li-Qin, ZUO Zhen-Tao, WANG Jing, FU Tao and CHEN Lin 20true 2018/6/20 0:00:00 SUN Ming-Xuan, ZHAO Rong-Tao, LI Yang, KONG Wen, YANG Yi, GUO Xu-Dong, LIU Wan-Ying, SONG Hong-Bin, ZHANG Zhi-Hong and HAO Rong-Zhang 19true 2018/4/17 0:00:00 ZHANG Xian-Chang, XUE Rong and ZUO Zhen-Tao 18true 2018/10/22 0:00:00 CHU Wen-Dan, XU Yang, ZHOU Cui-Yan, LU Ya-Fei, YU Xiao-Xia, ZHANG Rui-Xuan and LI Wen-Qi 17true 2019/7/1 0:00:00 YAO Qin,ZHANG Yu-Ying,WU Kai-Yuan,CHU Bo-Yu and CHEN Chang 16true Aedes aegypti is a pivotal vector for the dengue virus and expresses more than 30 types of C-type lectins, which are critical for both viral and bacterial infections. A recent study indicates that mosGCTL-3 interacts with a dengue virus-2 envelope protein (DENV-2 E protein) in vivo and in vitro thereby enhancing the infection of A. aegypti. Here, in this report we found another C-type lectin protein, mosGCTL-2 with important functions similar to mosGCTL-3. Results from the phylogenetic tree analysis suggested that there is sequence similarity between mosGCTL-2 and mosGCTL-3, with 43.56% of their amino acid sequences being identical. We constructed Drosophila S2 cell expression system to purify mosGCTL-2. In vitro experiments showed that mosGCTL-2 binds to the DENV-2 E protein through a calcium-dependent manner. The upregulation of mosGCTL-2 was noted in A. aegypti and was important for dengue virus 2 (DENV-2) infection in vivo. These findings suggest that mosGCTL-2 may be a pattern recognition receptor that performs an important function in the infection of A. aegypti by the dengue virus.]]> 2020/4/23 0:00:00 WU Yu-Jie,LIU Shan,ZHANG Xi,LI Dong-Yang,WU Yu-Lan,SUN Jing-Chuan,XU Shao-Jian,REN Yan,WANG Tao,LIU Li and GONG Jun-Yuan 15true HECT) at 3.2 ? resolution is reported, and structural comparisons of AREL1^HECT against different HECT E3 ligases are conducted. Size Exclusion Chromatography (SEC) and Small Angle X-ray Scattering (SAXS) indicate that there are diverse oligomeric states of AREL1^HECT in solution, and the SAXS 3D model further suggests that AREL1^HECT can dimerize in solution. These findings offer a structural basis for studying the complex of AREL1^HECT and ubiquitin, and provide insights into molecular mechanisms of substrate ubiquitination by AREL1.]]> 2020/8/11 0:00:00 LI Zhi-Hui,SHANG Guo-Hui,TANG Chen-Jun,TIAN Zi-Zi,WU Wei and CHEN Zhong-Zhou 14true 2021/3/4 0:00:00 MU Cheng-Long,HE Li-Qun,WANG Jia-Le,ZHAO Tian,ZHU Yu-Shan and CHEN Quan 13true 6A modification and the regulation of alternative splicing (AS). However, how RBM15 regulates AS is currently unclear. Here, using super-resolution microscopy, we found that RBM15 forms puncta structures that closely contact with or even embedded in the nuclear speckles. Nuclear speckles are enriched in splicing factors, which implies that RMB15 might be involved in RNA AS. To determine whether and how RBM15 regulates AS, we knocked down RBM15(RBM15-KD) using siRNA and performed RNA-seq for wildtype(WT) cells and RBM15-KD cells. We analyzed the RNA-seq of WT and RBM15-KD cells. We show that RBM15-KD cause 1 279 differential AS events in 1 111 transcripts. After comparing to public RBM15-CLIP data, we identify that 191 out of 1 111 transcripts are directly bound by RMB15, indicating that these 191 transcripts are probably direct targets of RBM15. Moreover, RBM15 promotes the retention of the adjacent regions proximal to its binding sites in 121 out of the 191 transcripts. This study reveals that how RBM15 regulates AS on a transcriptomic level.]]> 2021/6/24 0:00:00 WANG Ru,KE Yan,WEI Rong-Fei,WANG Xin-Yu and LI Dong 12true 2O) on germination and culturability of bacterial spore. Germination was triggered by L-alanine under ambient condition and in situ monitored by Tb³⁺-dipicolinic acid assay, phase contrast microscopy and absorbance measurement. Germination kinetics were characterized by final germination level, germination half time, and germination speed. The culturability of germinated spores was evaluated by enumerating colony-forming units. Results revealed that D₂O retarded the whole germination process and reduced the culturability but didn’t affect the final germination level. We postulate that D₂O enhanced the stability of some spore-specific signaling proteins.]]> 2021/11/26 0:00:00 CHANG Jinhui and WU Wen Jie 11true Objective Rheotaxis, namely to orient and swim against the water flow, is a conserved behavior across most fish and amphibians. While the study of rheotaxis behavior has a relatively long history, and in recent years the behavioral algorithm of rheotaxis has been described, how distributed neural circuits integrate multisensory information, make decisions, and generate counterflow motor sequences remain largely unknown. Whole brain calcium imaging of larval zebrafish during rheotaxis would provide a unique opportunity to tackle this difficult problem.Methods To this end, we developed a microfluidic device that can precisely control the water flow and elicit rheotaxis behavior. By integrating the chip with a customized light field tracking microscope, we built a system to record whole brain neural activity in freely behaving larval zebrafish during rheotaxis.Results Larval zebrafish showed reliable rheotaxis behavior in the setup, represented by prominent positional holding and counterflow swimming bouts in water flow. In the meanwhile, we successfully recorded zebrafish whole brain neural activity, from which a few brain regions were identified whose calcium signals strongly correlated with rheotaxis behavior.Conclusion Our study, for the first time, demonstrates a method for imaging whole brain neural activity in larval zebrafish while the animal is performing rheotaxis. Future analysis and modelling of the neural activity and behavioral data will deepen our understanding of sensorimotor transformation in this important naturalistic behavior.]]> 2022/9/21 0:00:00 WU Yu-Bin,ZHANG Ren-Chang,LI Da-Guang,QI Ke-Xin,CHAI Yu-Ming,SHEN Chen,SI Guang-Wei and WEN Quan 10true Objective DNA end resection is a common mechanism for the formation of 3"-ssDNA tails in homologous recombination (HR) and is mainly mediated by 5"-3" exonuclease. However, whether DNA end configurations directly regulate 5"-3" exonuclease activity remains unclear. In this study, we explored the regulation and mechanisms of DNA end configurations on RecJ, the only 5"-3" exonuclease of RecF recombination pathway in Escherichia coli.Methods To investigate the regulation of DNA 3"-end configurations on RecJ exonuclease, single-stranded DNAs (ssDNAs) containing different lengths of 3"-ssDNA overhangs (9 nt and 18 nt) and 3"-end modifications (phosphorylation and phosphorothioation) were used for exonuclease assays in the presence of Mg²⁺. To elucidate the mechanisms, RecJ was incubated with substrates containing different 3"-end configurations in the absence of Mg²⁺ and analyzed by electrophoretic mobility shift assays (EMSA). Furthermore, the coordination of DNA 3" end configurations and two other RecJ regulatory factors, DNA 5"-end phosphorylation and single-stranded DNA binding protein (SSB), were determined by exonuclease assays and EMSA on substrates with different 3"-end configurations respectively.Results DNA 3"-end configurations inhibited the RecJ exonuclease activity, including DNA 3"-overhang length and 3"-end modifications (phosphorylation and phosphorothioation). 3"-End phosphorylation and phosphorothioation of DNA reshaped the RecJ-DNA binding patterns to inhibit RecJ exonuclease activity. DNA 5"-end phosphorylation overcame RecJ inhibition of 3"-end modifications and remodeled the RecJ-DNA binding patterns. In addition, SSB partially overcame the 3"-end modifications mediated inhibition by enhancing RecJ-DNA binding.Conclusion The RecJ exonuclease activity was regulated and orchestrated by the DNA configurations of 3" and 5" ends.]]> 2022/6/21 0:00:00 MA Lan-Zhi,XIE Yan,ZHANG Peng,CHEN Hou-Zao and LIU De-Pei 9true Objective This study aims to explore the effects and mechanisms of LBP1C extracted from Lycium barbarum on delaying the natural aging of human fibroblasts and nematodes, and provide scientific evidence and explanation for the anti-aging and skin whitening effects recorded in the “Ben Cao Gang Mu (Compendium of Materia Medica)”.Methods Human fibroblasts and C. elegans were used as natural aging models and treated with LBP1C. The expression levels of P16, P21 and the number of SA-β-gal staining positive cell were used to indicate the cell senescence level. The levels of GATA4, indicators related to the senescence associated secretory phenotype (SASP), transcription factor EB (TFEB) targeting genes and autophagy marker protein LC3 were detected by qPCR or Western-blot. The level of nuclear transcription of TFEB, a key regulatory factor for lysosomal biogenesis and autophagy, was detected by immunofluorescence in fibroblasts. The effects of LBP1C on lipofuscin levels in fibroblasts and C. elegans were respectively detected by lipofuscin kit and fluorescence microscope. The motor ability of LBP1C to C. elegans was evaluated through body bends and pumping analysis.Results Human fibroblasts and C. elegans were used as natural aging models in this study. Aging related indicators (P16, P21 expression levels and the number of SA-β-gal staining positive cell) in the LBP1C treatment group decreased markedly compared with the control group, which indicated that LBP1C delayed cell senescence. The proportion of TFEB nuclear translocation significantly increased in the LBP1C treated groups and activated autophagy. Detection of TFEB downstream genes showed that mRNAs of lysosomal enzymes, lysosomal membrane proteins and functionally related vesicular ATPases were all significantly upregulated in the LBP1C treatment groups. The increase in autophagy level autophagy reduced the level of SASP transcription factor GATA4, thereby reducing the levels of SASP related IL-1β and iNOS. Besides, the increase in autophagy level accelerated the degradation of lipofuscin, and reduced the accumulation of lipofuscin in cells and C. elegans. LBP1C reduced the accumulation of lipofuscin in cells and C. elegans and improved the motor ability of C. elegans, which suggested that LBP1C promotes healthy aging of C. elegans.Conclusion This study investigated the new function and mechanism of LBP1C extracted from Lycium barbarum, which delays cell senescence by promoting autophagy to achieve the effects of anti-aging and skin whitening. LBP1C increased autophagy level by activating TFEB, thereby reducing the accumulation of SASP and lipofuscin, delaying aging and promoting healthy aging. This study reveals the effects and mechanisms of Lycium barbarum in anti-aging and skin whitening aspects, and provides scientific and theoretical basis for further application research, which provides exciting translational opportunities ahead.]]> 2023/8/14 11:14:21 WU Dong-Li,QIAO Xin-Hua,HAN Wen-Sheng,XIE Ting,SHI Chang,HUANG Yu-Yun-Fei,SUN Chuan-Xin,DING Kan and CHEN Chang 8true Objective Acetylcholine is a highly conserved neurotransmitter that plays a crucial role in the regulation of animal motor behavior. Abnormalities in acetylcholine signaling can lead to various motor dysfunctions. However, the inhibitory regulatory mechanisms of acetylcholine in motor behavior are not fully understood. In this study, we used Caenorhabditis elegans as a model organism to investigate the regulatory effects of acetylcholine-gated chloride channel receptor subunits (ACC-1, ACC-2, ACC-3, ACC-4) on motor behavior.Methods We used a combination of locomotion tracking, molecular genetics, and optogenetics to analyze C. elegans locomotion in acetylcholine-gated chloride channel subunit deficient mutants.Results We found that mutations in these subunits affected the kinematics of forward, backward, and turning movements of nematodes. The body bending amplitude during forward movement was also modified. Optogenetic activation of RIB interneurons led to delayed termination of the reversal in these mutant strains.Conclusion These results suggest that the regulation of acetylcholine-gated chloride channel subunits is required for maintaining and modulating C. elegans motor states. They also suggest that these subunits may be involved in mediating the inhibitory regulation of RIB interneurons on backward movement in C. elegans. This study provides new insights into the regulatory mechanisms of acetylcholine-gated inhibitory receptors in motor behavior.]]> 2023/6/20 16:49:48 Mahiber Polat,XIONG Rong-Kang,HUO Jing and WEN Quan 7true Objective Quercetin, a flavonol compound widely distributed in fruits, vegetables, and medicinal plants, has been suggested to act as a neuroprotective agent. In the present study, we investigated the presynaptic effect of quercetin on synaptic transmission and plasticity.Methods Using whole-cell patch clamp and capacitance measurement technique, we recorded miniature excitatory postsynaptic currents (mEPSC), presynaptic calcium influx, vesicle release and recycling, and the replenishment of readily releasable pool (RRP). Additionally, we stimulated the axon with 5-200 Hz and recorded short-term depression (STD) in the postsynaptic neuron.Results We found that 100 μmol/L quercetin in the extracellular solution did not affect the mEPSC amplitude and frequency, indicating a presynaptic mechanism modulating synaptic transmission. At the presynaptic nerve terminals, 100 μmol/L quercetin did not induce notable changes in calcium influx or vesicle release, but significantly inhibited clathrin-dependent slow endocytosis following exocytosis. The inhibition of endocytosis led to a slowdown of vesicle mobilization during stimulation, a reduction in readily releasable pool replenishment after stimulation, and enhancement of short-term depression during high-frequency repetitive stimulation in the postsynaptic principal neurons.Conclusion These results provide new insights into quercetin-modulated neuronal signaling and suggest a protective effect that prevents excessive excitatory synaptic transmission in brain circuits.]]> 2023/6/20 16:50:07 LI Shun,GAO Yi-Ming,XU Yue,HU Jia-Qi,TANG Wen-Xu,SUN Xiao-Quan,XUE Lei and WANG Wan-Chun 6true Objective Protein arginine methyltransferases (PRMTs) play pivotal roles in numerous cellular biological processes. However, the precise regulatory effects of PRMTs on the fate determination of mesenchymal stromal/stem cells (MSCs) remain elusive. Our previous studies have shed light on the regulatory role and molecular mechanism of PRMT5 in MSC osteogenic differentiation. This study aims to clarify the role and corresponding regulatory mechanism of PRMT7 during the adipogenic differentiation of bone marrow-derived mesenchymal stem cells (BMSCs).Methods (1) Human bone marrow-derived mesenchymal stem cells (hBMSCs) were cultured in a medium that induces adipogenesis. We used qRT-PCR and Western blot to monitor changes in PRMT7 expression during adipogenic differentiation. (2) We created a cell line with PRMT7 knocked down and assessed changes in PRMT7 expression and adipogenic capacity using Oil Red O staining, qRT-PCR and Western blot. (3) We implanted hBMSCs cell lines mixed with a collagen membrane subcutaneously into nude mice and performed Oil Red O staining to observe ectopic lipogenesis in vivo. (4) A cell line overexpressing PRMT7 was generated, and we examined changes in PRMT7 expression using qRT-PCR and Western blot. We also performed Oil Red O staining and quantitative analysis after inducing the cells in lipogenic medium. Additionally, we assessed changes in PPARγ expression. (5) We investigated changes in insulin-like growth factor 1 (IGF-1) expression in both PRMT7 knockdown and overexpressing cell lines using qRT-PCR and Western blot, to understand PRMT7’s regulatory effect on IGF-1 expression. siIGF-1 was transfected into the PRMT7 knockdown cell line to inhibit IGF-1 expression, and knockdown efficiency was confirmed. Then, we induced cells from the control and knockdown groups transfected with siIGF-1 in lipogenic medium and performed Oil Red O staining and quantitative analysis. Finally, we assessed PPARγ expression to explore IGF-1’s involvement in PRMT7’s regulation of adipogenic differentiation in hBMSCs.Results (1) During the adipogenesis process of hBMSCs, the expression level of PRMT7 was significantly reduced (P<0.01). (2) The adipogenic differentiation ability of PRMT7 knockdown group was significantly stronger than that of control group (P<0.001). (3) The ectopic adipogenic differentiation ability of PRMT7 knockdown group was significantly stronger than that of control group. (4) The adipogenic differentiation ability of the PRMT7 overexpression group was significantly weaker than that of the control group (P<0.01). (5) The expression level of IGF-1 increased after PRMT7 knockdown (P<0.000 1). The expression level of IGF-1 decreased after PRMT7 overexpression (P<0.000 1), indicating that PRMT7 regulates the expression of IGF-1. After siIGF-1 transfection, the expression level of IGF-1 in all cell lines decreased significantly (P<0.001). The ability of adipogenic differentiation of knockdown group transfected with siIGF-1 was significantly reduced (P<0.01), indicating that IGF-1 affects the regulation of PRMT7 on adipogenic differentiation of hBMSCs.Conclusion In this investigation, our findings elucidate the inhibitory role of PRMT7 in the adipogenic differentiation of hBMSCs, as demonstrated through both in vitro cell-level experiments and in vivo subcutaneous transplantation experiments conducted in nude mice. Mechanistic exploration revealed that PRMT7’s regulatory effect on the adipogenic differentiation of hBMSCs operates via modulation of IGF-1 signaling pathway. These collective findings underscore PRMT7 as a potential therapeutic target for fatty metabolic disorders, thereby offering a novel avenue for leveraging PRMT7 and hBMSCs in the therapeutic landscape of relevant diseases.]]> 2024/7/30 17:10:40 GUO Qian,QING Jia,LU Da-Zhuang,WANG Xu,LI Yang,ZHANG Hui,ZHANG Ying-Fei,LIU Yun-Song,ZHOU Yong-Sheng and ZHANG Ping 5true Objective Neuroinflammation plays a crucial role in both the onset and progression of ischemic stroke, exerting a significant impact on the recovery of the central nervous system. Excessive neuroinflammation can lead to secondary neuronal damage, further exacerbating brain injury and impairing functional recovery. As a result, effectively modulating and reducing neuroinflammation in the brain has become a key therapeutic strategy for improving outcomes in ischemic stroke patients. Among various approaches, targeting immune regulation to control inflammation has gained increasing attention. This study aims to investigate the role of in vitro induced regulatory T cells (Treg cells) in suppressing neuroinflammation after ischemic stroke, as well as their potential therapeutic effects. By exploring the mechanisms through which Tregs exert their immunomodulatory functions, this research is expected to provide new insights into stroke treatment strategies.Methods Naive CD4⁺ T cells were isolated from mouse spleens using a negative selection method to ensure high purity, and then they were induced in vitro to differentiate into Treg cells by adding specific cytokines. The anti-inflammatory effects and therapeutic potential of Treg cells transplantation in a mouse model of ischemic stroke was evaluated. In the middle cerebral artery occlusion (MCAO) model, after Treg cells transplantation, their ability to successfully migrate to the infarcted brain region and their impact on neuroinflammation levels were examined. To further investigate the role of Treg cells in stroke recovery, the changes in cytokine expression and their effects on immune cell interactions was analyzed. Additionally, infarct size and behavioral scores were measured to assess the neuroprotective effects of Treg cells. By integrating multiple indicators, the comprehensive evaluation of potential benefits of Treg cells in the treatment of ischemic stroke was performed.Results Treg cells significantly regulated the expression levels of both pro-inflammatory and anti-inflammatory cytokines in vitro and in vivo, effectively balancing the immune response and suppressing excessive inflammation. Additionally, Treg cells inhibited the activation and activity of inflammatory cells, thereby reducing neuroinflammation. In the MCAO mouse model, Treg cells were observed to accumulate in the infarcted brain region, where they significantly reduced the infarct size, demonstrating their neuroprotective effects. Furthermore, Treg cell therapy notably improved behavioral scores, suggesting its role in promoting functional recovery, and increased the survival rate of ischemic stroke mice, highlighting its potential as a promising therapeutic strategy for stroke treatment.Conclusion In vitro induced Treg cells can effectively suppress neuroinflammation caused by ischemic stroke, demonstrating promising clinical application potential. By regulating the balance between pro-inflammatory and anti-inflammatory cytokines, Treg cells can inhibit immune responses in the nervous system, thereby reducing neuronal damage. Additionally, they can modulate the immune microenvironment, suppress the activation of inflammatory cells, and promote tissue repair. The therapeutic effects of Treg cells also include enhancing post-stroke recovery, improving behavioral outcomes, and increasing the survival rate of ischemic stroke mice. With their ability to suppress neuroinflammation, Treg cell therapy provides a novel and effective strategy for the treatment of ischemic stroke, offering broad application prospects in clinical immunotherapy and regenerative medicine.]]> 2025/2/28 16:13:08 KANG Tian-Fang,MA Ai-Qing,CHEN Li-Qi,GONG Han,OUYANG Jia-Cheng,PAN Fan,PAN Hong and CAI Lin-Tao 4true Objective Cerebral palsy (CP) is a prevalent neurodevelopmental disorder acquired during the perinatal period, with periventricular white matter injury (PWMI) serving as its primary pathological hallmark. PWMI is characterized by the loss of oligodendrocytes (OLs) and the disintegration of myelin sheaths, leading to impaired neural connectivity and motor dysfunction. Neural stem cells (NSCs) represent a promising regenerative source for replenishing lost OLs; however, conventional two-dimensional (2D) in vitro culture systems lack the three-dimensional (3D) physiological microenvironment. Microfluidic chip technology has emerged as a powerful tool to overcome this limitation by enabling precise spatial and temporal control over 3D microenvironmental conditions, including the establishment of stable concentration gradients of bioactive molecules. Catalpol, an iridoid glycoside derived from traditional medicinal plants, exhibits dual antioxidant and anti-apoptotic properties. Despite its therapeutic potential, the capacity of catalpol to drive NSC differentiation toward OLs under biomimetic 3D conditions, as well as the underlying molecular mechanisms, remains poorly understood. This study aims to develop a microfluidic-based 3D biomimetic platform to systematically investigate the concentration-dependent effects of catalpol on promoting NSCs-to-OLs differentiation and to elucidate the role of the caveolin-1 (Cav-1) signaling pathway in this process.Methods We developed a novel multiplexed microfluidic device featuring parallel microchannels with integrated gradient generators capable of establishing and maintaining precise linear concentration gradients (0-3 g/L catalpol) across 3D NSCs cultures. This platform facilitated the continuous perfusion culture of NSC-derived 3D spheroids, mimicking the dynamic in vivo microenvironment. Real-time cell viability was assessed using Calcein-AM/propidium iodide (PI) dual staining, with fluorescence imaging quantifying live/dead cell ratios. Oligodendrocyte differentiation was evaluated through quantitative reverse transcription polymerase chain reaction (qRT-PCR) for MBP and SOX10 gene expression, complemented by immunofluorescence staining to visualize corresponding protein changes. To dissect the molecular mechanism, the Cav-1-specific pharmacological inhibitor methyl-β-cyclodextrin (MCD) was employed to perturb the pathway, and its effects on differentiation markers were analyzed.Results Catalpol demonstrated excellent biocompatibility, with cell viability exceeding 96% across the entire tested concentration range (0-3 g/L), confirming its non-cytotoxic nature. At the optimal concentration of 0-3 g/L, catalpol significantly upregulated both MBP and SOX10 expression (P<0.05, P<0.01), indicating robust promotion of oligodendroglial differentiation. Intriguingly, Cav-1 mRNA expression was progressively downregulated during NSC differentiation into OLs. Further inhibition of Cav-1 with MCD further enhanced this effect, leading to a statistically significant increase in OL-specific gene expression (P<0.05, P<0.01), suggesting Cav-1 acts as a negative regulator of OLs differentiation.Conclusion This study established an integrated microfluidic gradient chip-3D NSC spheroid culture system, which combines the advantages of precise chemical gradient control with physiologically relevant 3D cell culture. The findings demonstrate that 3 g/L catalpol effectively suppresses Cav-1 signaling to drive NSC differentiation into functional OLs. This work not only provides novel insights into the Cav-1-dependent mechanisms of myelination but also delivers a scalable technological platform for future research on remyelination therapies, with potential applications in cerebral palsy and other white matter disorders. The platform’s modular design permits adaptation for screening other neurogenic compounds or investigating additional signaling pathways involved in OLs maturation.]]> 2025/11/5 11:41:42 WANG Ya-Chen,WANG Liang,SHEN Li-Ming and LIU Jing 3true Objective Post-ischemic acute inflammation and the subsequent persistent dysregulation of the immune microenvironment represent major pathological drivers that aggravate neuronal injury and severely restrict functional recovery following ischemic stroke. Although current reperfusion therapies partially restore blood flow, they fail to effectively modulate the secondary inflammatory cascade and oxidative stress, which remain critical barriers to neurological restoration. To address this challenge, this study aimed to engineer and systematically evaluate a biomimetic nanosystem composed of transforming growth factor-β1 (TGF-β1)-loaded platelet membrane-camouflaged lipid nanoparticles (PLP). This nanosystem was designed to achieve dual lesion-targeted delivery and immune microenvironment remodeling. By verifying its spatiotemporal accumulation, anti-inflammatory activity, and neuroprotective efficacy, we sought to establish an integrated therapeutic strategy that simultaneously enables lesion targeting, immune regulation, and functional recovery after ischemic injury.Methods The physicochemical properties of PLP, including hydrodynamic particle size, zeta potential, structural stability, and morphology, were characterized using dynamic light scattering, zeta potential analysis, and transmission electron microscopy. The preservation of platelet membrane-derived adhesion and immunoregulatory proteins was confirmed by SDS-PAGE through comparative analysis of protein band profiles between PLP and native platelet membranes. The in vitro biological activities of PLP were evaluated using two complementary cellular models. LPS-induced M1-polarized RAW264.7 macrophages were employed to assess inflammatory modulation, while oxygen glucose deprivation/reperfusion (OGD/R)-induced BV2 microglial cells and SH-SY5Y neuronal cells were utilized to investigate neuroinflammatory regulation and neuronal protection. For in vivo validation, a transient middle cerebral artery occlusion (tMCAO) mouse model was established to mimic ischemia-reperfusion injury. The spatiotemporal biodistribution and lesion-targeting capability of the PLP were monitored through live fluorescence imaging. Therapeutic efficacy was comprehensively evaluated by triphenyltetrazolium chloride (TTC) staining, glial fibrillary acidic protein (GFAP) immunofluorescence analysis, body weight monitoring, and neurological severity score (NSS) assessment.Results PLP nanoparticles displayed a uniform spherical morphology, nanoscale particle size distribution, and stable negative surface charge, indicating favorable colloidal stability and circulation potential. SDS-PAGE results confirmed the effective retention of key platelet membrane proteins associated with endothelial adhesion, immune evasion, and inflammatory regulation, demonstrating the successful biomimetic construction. Optimal therapeutic concentrations were determined in OGD/R-induced BV2 cells, where PLP exhibited excellent cytocompatibility and anti-inflammatory activity. In vitro experiments demonstrated that PLP significantly inhibited the polarization of RAW264.7 macrophages toward the pro-inflammatory M1 phenotype and markedly reduced neuronal apoptosis under ischemia-reperfusion conditions. In vivo fluorescence imaging revealed that PLP rapidly accumulated in the ischemic brain hemisphere and maintained prolonged retention for up to 7 d, suggesting enhanced lesion-specific targeting and sustained drug release. Compared with control group, PLP treatment significantly reduced cerebral infarct volume, attenuated reactive astrogliosis, improved weight recovery, and accelerated neurological functional restoration, as reflected by significantly improved NSS scores.Conclusion This study establishes a multifunctional biomimetic nanoplatform that integrates platelet membrane-mediated active targeting with the anti-inflammatory, antioxidative, and neuroprotective properties of TGF-β1. The PLP system enables rapid lesion homing and long-term retention while synergistically regulating the post-stroke inflammatory microenvironment by suppressing pro-inflammatory immune activation, reducing neuronal apoptosis, and limiting excessive astrocyte reactivity. Importantly, this study proposes a conceptually therapeutic paradigm that combines targeted delivery with immune microenvironment remodeling to achieve comprehensive neurovascular protection. These findings provide strong experimental evidence supporting the translational potential of biomimetic nanotherapeutics as next-generation precision interventions for ischemic stroke.]]> 2026/2/7 8:22:53 CHEN Li-Qi,KANG Tian-Fang,HUANG Guo-Jun,YIN Ting,MA Ai-Qing,CAI Lin-Tao and PAN Hong 2true Objective Pancreatic ductal adenocarcinoma (PDAC) exhibits a limited response to current treatments due to its dense fibrotic stroma and highly immunosuppressive tumor microenvironment. In recent years, advancements in cellular immunotherapy, particularly chimeric antigen receptor macrophage (CAR-M) therapy, have offered new hope for pancreatic cancer treatment. Although CAR-M therapy demonstrates dual potential in directly killing tumor cells and remodeling the immune microenvironment, it still faces challenges such as complex in vitro preparation processes and low in vivo targeting and delivery efficiency. Therefore, developing strategies for efficient and targeted in vivo delivery of CAR genes has become crucial for overcoming current therapeutic limitations. This study aims to develop an orally administrable nano-gene delivery system for the targeted delivery of CAR genes to pancreatic tumor sites.Methods Core nano-gene particles (PNP/pCAR) were constructed by loading plasmid DNA encoding CAR (pCAR) with cationic polypeptides (PNP). Subsequently, PNP/pCAR was surface-modified with β-glucan to prepare the targeted nanoparticles (βGlus-PNP/pCAR). The loading efficiency of PNP for pCAR was quantitatively assessed by gel retardation assay. The particle size, Zeta potential, morphology, and storage stability of PNP/pCAR were characterized using a Malvern particle size analyzer and transmission electron microscopy. At the cellular level, RAW 264.7 macrophages were selected. The cytotoxicity of PNP/pCAR was evaluated using the CCK-8 assay. The cellular uptake efficiency and lysosomal escape ability of the nanoparticles were assessed via flow cytometry and confocal microscopy. Transfection efficiency was quantitatively evaluated by detecting the expression of the reporter gene GFP using flow cytometry. At the in vivo level, an orthotopic pancreatic cancer mouse model was established. Cy7-labeled βGlus-PNP/pCAR nanoparticles were administered orally, and the fluorescence distribution in mice was dynamically monitored at 1, 2, 4, 8, and 16 h post-administration using a small animal in vivo imaging system. Forty-eight hours after oral gavage, the mice were euthanized, and pancreatic tumor tissues were collected for further analysis of intratumoral fluorescence signals using the imaging system. Additionally, βGlus-PNP/pCAR-GFP nanoparticles loaded with the reporter gene (GFP) were administered orally. Forty-eight hours post-administration, pancreatic tumor tissues were harvested to prepare frozen sections, and GFP expression was observed and analyzed under a fluorescence microscope.Results The PNP carrier exhibited a high loading capacity for pCAR. The successfully prepared PNP/pCAR nanoparticles were regular spheres with a hydrodynamic diameter of approximately (120±10) nm and a Zeta potential of about +(6±1) mV. They maintained good structural stability after incubation in PBS buffer for 7 d. Cell experiments demonstrated that PNP/pCAR exhibited no significant cytotoxicity in RAW 264.7 cells while being efficiently internalized and effectively escaping lysosomal degradation. The transfection positive rate of PNP/pCAR-GFP in RAW 264.7 cells reached (25±3)%, surpassing that of Lipofectamine 2000-loaded pCAR-GFP (Lipo/pCAR-GFP), which was (20±1)%. In vivo experiments revealed that, compared to unmodified PNP/pCAR, βGlus-PNP/pCAR exhibited stronger in situ pancreatic tumor targeting ability after oral administration. Furthermore, oral administration of βGlus-PNP/pCAR-GFP resulted in significant GFP protein expression detectable within pancreatic tumor tissues.Conclusion This study successfully constructed and validated an orally administrable, pancreatic cancer-targeting polypeptide-based nano-gene delivery system. It provides an important technological foundation in delivery systems and experimental basis for the subsequent development of in situ CAR-M-based therapeutic strategies for pancreatic cancer.]]> 2026/1/14 10:00:02 XIN Feng,REN Jian,LI Zhao-Zhen,FANG Quan,LIANG Rui-Jing,LIU Lan-Lan and CAI Lin-Tao 1true