贺宝珍,徐秀璋 273true 季钟煜,李文杰 272true 李桂琴,冯淑娱 271true 徐秀璋 270true 白应林 269true 景沛,苏妙根 268true 贺宝珍 267true 邹福强,梁意昭,王义良,曾淑云 266true 雷克健 265true 鲁卓卿,孙凤至,刘丽文,刘淑霞 264true 李积义 263true 江寿平,乐树云 262true 韩恒湘,程振起 261true 张向明 260true 静国忠 259true 胡云梯,童秀珍 258true 赵德永,周鑫生 257true 李生广,孙珊 256true 李成文,时华富,邵军石 255true 程寿宜,周炳森,张纪宁,万福生,温博贵,郭菊美 254true 周德和,倪崇虎,李志毅,池志强 253true 逯建英 252true 张林元,王祁 251true 许力伟,姚根富,瞿永华 250true 夏志清,陈农安 249true 袁铸人 248true 逯建英 247true 汪淑华,朱应麒 246true 林奋智,沈德福 245true 彭兴华,李桐芬,朱尚权 244true 邵嘉兴,田湘勇 243true 徐佐杰,俞鹤年 242true 胡匡祜 241true 邱长春,周文郁,吴冠芸,王荣新,张尼佳,黄有文 240true 潘苏华 239true 司世麟 238true 蒋立科 237true 李志毅,周德和,倪崇虎,池志强 236true 王玉梅,丛培红,刘存英 235true 夏志清 234true 李竹,丁锡申,徐康森 233true 石志远 232true 董景明,赵天睿 231true 胡军,周兆康 230true 邱长春,吴冠芸,吴志奎 229true 俞鹤年,张国娣 228true 李琪 227true 萧惠连 226true 刘定干,李载平 225true 于立平,季坤之 224true 章蓓,倪大洲,刘祖洞 223true 朱展才 222true 陈去恶,周启玲,邢国仁,郑德存,柳青梅 221true 陈崇光,郭秀月,赵国栋,奚国良 220true 池旭生,庞大本 219true 任惠民,章生艮 218true 郑木基,池旭生,庞大本 217true 严家新,林乔,王懋梁 216true 丁振英,郑秀龙 215true 马延高,陈蔚梅,王静怡,燕小安 214true 何照范,单友谅,牛爱珍 213true 尚克进,凌启阆,李翠凤,曹兢,胡卫鲤 212true 王育东 211true 赵永芳,朱勤 210true 郑珉,杨胜利,邹冈 209true 陈海鑫,李振甲 208true 梁念慈,康景轩 207true 袁铸人 206true 陈曦,陈兰英 205true 周锡漳,刘琳 204true 薛建华 203true 钟菊香 202true 吴芝清,王银定,任向宇 201true 吴芝清,任向宇,宋陆铧 200true 滕斌 199true 聂崇兴,陈代雄 198true 韩紫杰,刘佑国,陈慎,华陵,宋桂云,王淑娟,刘仲昌 197true 刘运康,韦克,范维珂 196true 张向明 195true 胡望平,朱忠勇,倪正菱 194true 王辛中,周顺伍,齐顺章 193true 王国俊,戎积圻 192true 吴芝清,宋陆铧 191true 王华岩,贾锋 190true 许烨,黄桂芳,钱绍祯 189true 张林生,路苹,曹让 188true 赵永芳,周济兰,叶明 187true 张林生,路苹,曹让 186true 蒋作君,冯笑川,沈一平 185true 朱昌亮,叶炳辉,朱晓龙,赵学忠,张云 184true 叶明,周济兰 183true 朱晓龙,叶炳辉,朱昌亮,赵学忠,张云 182true 汪建 181true 赵宗建,赵宗英 180true 骆爱玲,黄巨富,梁寅初 179true 蔡继炯,朱锡林 178true 叶尚勉 177true 童兴龙 176true 康建,陈胜洪,曹思连,初俊杰 175true 逯斌,林兵 174true 赵晓瑜,静天玉 173true 邹辉琼,陈章林,邓水生 172true 尤小梅,唐文恒 171true 张晓,王厚行 170true 孙雅贤,魏丽君,曹军,王庭桂 169true 杨克俭,陈淑芬,盂宗达 168true 陈庆辉,梁念慈 167true 丛祥凤,张英珊 166true 饶春明,赵燕平,张向明,丁锡申 165true 张普民,李茂深 164true 范培昌 163true 王大坤 162true 张鲁榕,孔宪涛,张浩 161true 蒋作君 160true 谢继锋 159true 陈立业 158true 王厚行 157true 汪洪亮,吴从山 156true 曾福祥,刘光英,叶尚勉 155true 王尧 154true 沈萍 153true 陶家平,陈捷光 152true 陶钧 151true 韩刚毅 150true 白书农,王长举 149true 梁舜薇,潘苏华 148true 李清华,李慧萍,黄全荣 147true 韩刚毅,韩刚正 146true 张燕玲,张迺哲,符云峰 145true 凌俊 144true 龚成新 143true 陈冰铨,张鹏,姚淑珍,刘健 142true 杨于军,周辰,A.JONG 141true 周专,康华光 140true 方福德 139true 张利华,张志良,沈曾佑 138true 向荣,王鼎年 137true 张新波,崔肇春,朱正美 136true 杨学田,王纯青,徐以玲 135true 高志强 134true 朱元晓,陈勇,刘琛,白炎 133true 吴芝清,宋陆铧,任向宇 132true 周筠梅 131true 石志远,谈曼琪 130true 康建,初俊杰 129true 朱明华,刘彦仿 128true 陈文勇 127true 池木根,陈世铭 126true 钟海云,张佩华,刘丽娜,汪彤 125true 魏有仁,孙先高 124true 蔡望伟,凌光鑫,陈历昌 123true 刘建荣,赵晓瑜,静天玉 122true 徐明波,姚志建 121true 吴俊辉,舒煦 120true 杨正红,庄志雄,高梅,王敏 119true 李永兴,胡长征,王继文,李久蒂 118true 汪俊军,朱汉民 117true 程刚,孙大业 116true 李明举,汪洛 115true 邓碧玉,袁勤生,李文杰 114true 邹岳奇,阎静辉,刘玉翠 113true 丰美福 112true 张楚富 111true 汪宽蓉,孙彤,郭居潜,林燕 110true 潘苏华,梁舜薇 109true 何绮,陈淑华,尹钟洙 108true 董宝中,顾向明,生文君,张思和,石志远,谈曼琪 107true 李柏青,田志刚,刘杰,孙汭,张建华,张捷,崔正言 106true 张新波,汤乃梅,张席锦,程时 105true 刘钟瑸,杨贵贞,王文余 104true 阎小君,苏成芝,吉昌华 103true 丁克宏,梁念慈 102true 赵建钢,杨同书 101true 何彬,范明,甘思德 100true 陈拔盛,刘望夷 99true 杨银书,杜全木,卞启华,赵晓伯,王洁瑜 98true 杜建生,陈曦 97true 王梓壬,蔡玉奎 96true 徐晓平,陈志华,苏新 95true 武廷章 94true 金顺钱,张伟 93true 宋耀虹,李学仁,马德均,林其燧 92true 虞莉文,曹阳,刘亚铭,陶文洪,董旭东 91true 黄舒牧,郭蕾 90true 李国君,沈文梅,王成彬,邓心新,蒋赐恩 89true 吴晓强,李庚白 88true 陈惠,崔泓 87true 魏征宇,叶寅,田波 86true 王建华,朱正美 85true 纪学锋,章咏华 84true 方芳,潘华珍,潘延 83true 汤习锋,康格非,曾成鸣 82true 李红兵,李宗让,王志玲,孙连云 81true 王亚新,陆佩华,周光炎 80true 唐志毅,许维桂,杨振华,黄一明,毛利民,吴华 79true 丛建波,孙存普,莫简 78true 余伍忠,周常文,郝小军,魏静,李厚钧 77true 汪洛,张迺蘅 76true 汪洪亮 75true 张爱群,沈文梅 74true 王志珍 73true 黄亚军,徐媛 72true 朱世银,王红英,于建华,李锡贵 71true 叶淑金,陈小红 70true 李克,胡心宝 69true Shi Yanhong,Zhao Shimin and Sun Yongru 68true Zhang Changjing,Li Yihe and Li Xianbai 67true Zhang Jiquan,Chen Youchun,Zou Yueqi and Yan Jinghui 66true Yuan Suli,Fu Li and Zhang Qijiu 65true Shi Yuling,Chen Yanhong and Liu Xun 64true 2+ concentration ([Ca2+]i) was measured in isolated neurocytes prepared from Sprague-Dawley rats with Fura-2 double-wavelength fluoremetry. The results showed that the [Ca2+]i, of the resting neurocytes in Ca2+-containing solution was 109±12nmol/L(x±s, n=9).KCl (30mmol/L) markedly evoked [Ca2+] of the neurocytes (P＜0.01, n=9),and the rises of [Ca2+]i by KCl is a concentration-dependent response.These results suggest that the adoption of Fura-2 double-wavelength fluoremetry in dissociated rat neurocytes in a useful and relatively easily applicable technique for monitoring intracellular Ca2+ changes.]]> Lu Bufeng,Huang Yisen and Lu Youming 63true 1. MM2 and MM3 respectively.]]> Kong Qingyin,Yang Zhenhua,Yang Shude and Tang Zhiyi 62true In situ hybridization is a necessary and hard work for screening cDNA library.Heated by microwave oven,bacteriophage lysis,DNA denaturation and fixation of DNA to filter occur in a single step;On the other hand,it is a really rapid procedure for analysis of the cDNA inserts of positive clones via polymerase chain reaction (PCR) amplification.]]> Zhou Zhaolan,Zhu Zhen,Liu Chunming,Xiao Guifang and Li Xianghui 61true Cai Huiguo,Chen Peizhen,Zhang Lidong,Peng Qiong,Ji Xinjun and Ma Shuang 60true Bi Xiangjun,Peng Zhaohui,Li Jinhan,Yang Guangcai,Xu Xiangmin and Wang Hong 59true Li Changlong,Zhao Mancang,Wang Shu,Jiang Lei and Li Jianzhai 58true Xia Lingchao,Li Jinzhao and Chen Yan 57true Wang Junjun,Zao Zongnong,Zhuang Yiyi,Qiu Tinggang and Lu Wei 56true Wang Yilun,Dai Jianxin,Guo Yingjun and Lu Deru 55true + concentration. A new color-developing reagent formula which consists of CBB, HCl and NaCl, may improve the nonlinearity.]]> Guo Minliang and Jiang Yongming 54true p-nitrophenyl phosphate was used as substrate was improved by use of the terminating reagent containing 1 mol/L NaOH-0.2 mol/L EDTA. A better assay result was obtained.]]> Liang Zhiqun,Li Xiangping and Yang Shengyuan 53true r= 0.9906). Normal values in healthy adults was (694.97 ±102.31 ) μmol/L in male and (623.79±99.27) μmol/L in female with a significant difference (P＜0.05 ). The method is simple,efficient in performance and can be applied in the study of bowel structure and function and in routine measuements in nutritional support.]]> Liu Fangnan,Sheng Xueqin and Chen Yongming 52true Li Yingqiu,Shi Xianzong,Wu Wenyan,Long Qingxin and Wang Xunzhang 51true in situ electrophoretic size-fractionation". Furthermore, the glycerol at the final concentration of 20% is introduced directly into the medium used to grow the transformants, which would both simplify the operation and reduce the chance of contamination with other microorganisms or cross-contamination of YAC. clones. Such improved methods can be used in the construction and application of other genomic YAC libraries .]]> Yang Zhiyong,Kuang Daren,Li Lingli,Lu Shao,Song Minghao,Dai Heping,Long Zhigao,Pan Qian,Li Luyun and Xia Jiahui 50true Zhu Liuqin and Wu Yujie 49true Yang Kai and Shang Zhonglin 48true Jia Zhishen,Wu Jianmin and Tang Mengcheng 47true Li Xinquan,Zheng Min and Li Xinyue 46true Dong Sijian and Liu Changling 45true Mycoplasma pneumoniae, and reproducible result was acquired. The reported method can be used in molecular detection of clinical pathogens.]]> LIU Jirong,WU Wanfang,QIN Yuchun,ZHANG Yu and MA Guanfu 44true TAN Deyong,DENG Shuangsheng,MENG Ling and ZAN Ruiguang 43true LI Xiaopeng,DU Linfang,LIANG Houguo and WU Wansun 42true FAN Lingzhi,LIU Yanfang,YANG Xinke and HOU Yunde 41true PstⅠ and ligated with synthetic adapter.Selective amplification of DNA fragments （SADF） was performed using suitable primers which annealed to adapter regions.Agarose gel electrophoresis shown that DNA polymorphic fragments were appeared for both C108 and Dazao.]]> HE Ning-Jia,LU Cheng,ZHOU Ze-Yang and XIANG Zhong-Huai 40true 2 strain was amplifyed by reverse transcription and polymerase chain reaction (RT-PCR).HAV H2 was precipitated by anti-H2 serum specifically, then the RNA of HAV H2 was isolated from this precipitation by acid guanidinium hydrochloride-phenal-chloroform extraction. The first-strand of HAV H2 cDNA was synthesised by reverse transcriptase without RNase H activity,then was ampliflied by PCR using the 32mer primer and the Taq DNA polymerase with Deep Vent DNA polymerase. For obtaining longer PCR products,it is necessary to prepare high quality RNA and employ the longer primers and special Taq DNA polymerase.]]> ZHOU Xiang,TAN De-yong,LAI Jian-hua and ZAN Rui-guang 39true in vitro translation experiments performed with this RNA also gave good results.]]> HE Jun-xian and LIANG Hou-guo 38true LIAN De-jun and XU Gen-jun 37true QIN Wen-xin,WAN Da-fang,ZHAO Xin-tai,JIANG Hui-qiu and GU Jian-ren 36true HUANG Peng,MIAO Shi-ying and WANG Lin-fang 35true 2O,PBS-T and SDS. The results showed that this pretreatment led to the covalent attachment of CCK-8 to the solid-phase steadily and fit to different sources of microplates. The treated microplates could be stored at least 4 weeks not to influence their ability of binding antigens.The intra- and inter- variation coefficients were 4.75% and 7.80% respectively.This polypeptide ELISA was characterized by covalent attachment between antigen and solid-phase carrier and was proved to be highly stable,reproducable and available.]]> HE Tao and CHEN Man-Ling 34true HE Yuan-Zheng and LIU Ding-Gan 33true LI Yong,NI Fu-Tai and HE Fu-Chu 32true Tm) as an important parameter to identify DNA, which differs in sequence among a mixture of molecules of the same size but different conformation. With the advantages of high-resolution capability, high reproducibility and timesaving process, the TGGE technique has been applied broadly in the field of molecular biology.]]> CHEN Han-Kui and FENG Xin 31true -·2 produced NO-2 and then developed by sulphanilic acid and α-naphthylamine.The results indicated that there was a special absorption at 530 nm when the sample was tested by spectrophotometer, and its color responsiveness showed a quantitative relation to O-·2.Otherwise, ascorbic acid as a matter deleted oxygen radicals had a significantly quantitative relation too.]]> XIAO Hua-Shan,HE Wen-Jin,FU Wen-Qing,CAO Hong-Yun and FAN Zi-Nan 30true r=0.9824＞0.7800=r0.001,n=12). It suggested that this method of DNA assay would have great application value in study on follicular gowth and development.]]> LI Jian,KANG Jin-Quan,ZHOU Le and WANG Jian-Chen 29true ＋-ATPase from higher plants. The sensitivity of two common fluorescent dyes was compared. Acridine orange is better than quinacrine with the initial slope and maximum quenching. The optimim for the fluorescence measurement of acridine orange dye were:5 μmol/L acridine orange, 200～250 μg protein, Hepes-Tris of Hepes-BTP(pH 7.0). Initiating with ATP or Mg2＋ was both valid.]]> WANG Huan,ZHU Xiong-Wei and WANG Yan-Zhi 28true EcoRⅤ, then T-A cloning vector was prepared in the presence of Taq DNA polymerase and dTTP. β-actin cDNA fragment was cloned into the above T-A cloning vector. After the recombinant plasmids were digested by EcoRⅠ and HindⅢ, an expected size of β-actin cDNA fragment was obtained.]]> LI Xin-Bo,ZHAO Xiao-Song,TIAN De-Zhi,ZHU Yi-Chun and YAO Tai 27true CHEN Shu-Qing,SUN Hong-Ying,ZHAO Lu-Hang and P.J.WEDLUND 26true LIU Ding-Xie and LUO Kan-Xian 25true JIANG Nan,WU Kai-Li,HUANG Qiang and PAN Su-Hua 24true 3Fe(CN)6 into K4Fe(CN)6. Then K4Fe(CN)6 can react with FeCl3 resulting in Prussian blue which dyes LDH isozymes.]]> ZHAO Gan,ZHANG Shou-Quan,HUANG Xiao-Wu,ZHAN Fu-Jian and HE An-Xing 23true ZHOU Jian,WANG Xiao-Xing and GAO Xiao-Ping 22true XIA Qing-Jie,ZHANG Si-Zhong,WU Hui and XIAO Cui-Ying 21true HAN Mei,Lü Ping,HE Qi-Hua,YUAN Lan,MA Shi-Liang and TIAO Jia-Ping 20true ZHU Shun-Yi and LI Wen-Xin 19true 2 is reported. 3～10 min incubation of cells with DNA on ice and spreading onto agar plates pre-warmed to 37℃, resulting in more than 105 colonies/μg plasmid DNA. The effect of Ca2+ concentration and store time at 4℃ on transformation efficiency is also discussed.]]> XIAO Geng-Fu,QI Yi-Peng,LI Li and YI Wei 18true GUO Ze-Kun and ZHANG Yong 17true -1, the limit is 1.85 μmol·L-1. The RSDs are 2.56%～3.46%, and the recoveries are 96.4%～102.2%, 95.2%～101.3% and 98.7%～104.4% for NO-3, NO-2 and NO-3/NO-2 (95∶5) respectively. This method is easy, rapid and precise, needing only a small amount of sample and reagents, and can be used in clinical assay.]]> DENG Jian,Lü Chang-Yin,ZHANG Jian-Ming and YUAN Ya-Li 16true A630 with the clot lyses, the －A630 of many samples was detected at the same time with EL311 Auto Reader. It was found that the A630 declined linearly with time, and k under different dillutions could reflect the fibrinolytic activity of enzyme. This method costs less time, less reagent and correlated with CLT very well, indicating it was a good and useful method for determination of fibrinolytic enzymes.]]> XIE Qiu-Ling,LIN Jian and ZHANG Ling 15true A260 to A280 were greater than 1.85, the values of A230 decreased to an acceptable level which meant the contaminated carbohydrate was removed mostly from the preparations of total RNA. Bands of 28S RNA and 18S RNA could be seen apparently on the 1 % formaldehyde-agarose gel. Total RNA could be reverse transcripted into cDNA and β-actin and BMP-2 gene were successfully amplified by PCR. The results showed that the total bone tissue RNA was the qualified contemplate for RT-PCR.]]> GUO Ruo-Lin,GUO Shan-Yi,ZUO Ai-Jun,LIANG Dong-Chun and ZHANG Jing-Yu 14true XU Dong-Dong,LIU De-Pei,Lü Xiang,XU Hai-Ming,ZHANG Shen and LIANG Zhi-Quan 13true 2+，concentration of DNA, concentration of primers were reported. The results show that the optimum amplified condition of AP-PCR are 2 mmol/L MgCl2, 50 ng of DNA and 0.3 μmol/L of primers. Under these conditions the AP-PCR productions are richness and the most clear.]]> Askar Yimit,Halmurat Upur and Ilhamjan Sabit 12true ZHANG Zhen-Zhong,WU Yi-Ming,CHENG Wei-Ming and WU Ru-Jin 11true ZHANG Cheng-Gang,OUYANG Shu-Guang,ZHANG Shao-Wen,QU Xiang-Hu,YU Yong-Tao,ZHOU Gang-Qiao,WU Song-Feng and HE Fu-Chu 10true 4. The arms of IDA were chelated with Ni2+,and the chelating resin―Ni2+-IDA could be prepared. The physicochemical indexes and performance in purifying protein of the expressing product were assayed with atomic absorption method and purifying aimed protein-human B lymphocyte stimulator(hBLyS) from the expressing products in E.coli. The results indicated that the performance of made gel is very good, and its price is less than 1/10 of that of commodity gel.]]> LIU Ping,ZHANG Shuang-Quan,YAN Xiao-Mei and XU Jin-Shu 9true TAO Mei-Lin,ZHANG Yu-Qing,SHEN Wei-De,WANG Xin-Bo,ZHANG Hai-Ping,ZHOU Wen-Lin and MA Yan 8true 郑旭煦,胡应和,欧阳克清,李新平,蔡绍皙 7true SHEN Wen-Biao,ZHOU Tong,WANG Yi-Hua,YU Wei-Wei,ZHENG Tian-Qing,ZHAI Hu-Qu and WAN Jian-Min 6true WU Xue-Qin,WU Cen,LING Xiao-Hong,CHEN Jian-Wen and HE Rong-Qiao 5true LIU Ding-Xie,ZHOU Xiao-Wei and HUANG Pei-Tang 4true Ovomermis sinensis were successfully separated.]]> GAO Yuan,WANG Guo-Xiu and LIU Zhong-Lai 3true BAI Xiang-Yang,Lü An-Guo,WU Wen-Fang,SUN Jing and NIU Rui-Fang 2true WANG Kun,WANG Ying,BAO Yong-Li,MENG Xiang-Ying,WU Yin and LI Yu-Xin 1true