王广仪 111true 王广仪 110true 王广仪 109true 王广仪 108true 王广仪 107true 王广仪 106true 翟森,鲁柱 105true 翟森,鲁柱 104true 兰州生物制品研究所生化组 103true 兰州生物制品研究所生化组 102true 兰州生物制品研究所生化组 101true 兰州生物制品研究所生化组 100true 李治湘 99true 翟森,鲁柱 98true 李治湘 97true 王大成 96true 李家瑶 95true 王大成 94true 徐伟,门绍杰 93true 郭尧君 92true 何泽涌 91true 林万禄 90true 薛良琰,张仲伦 89true 敖世洲 88true 喻致祥,黄剑 87true 刘次全,石秀凡,王莹 86true 莫简 85true 鲁崎唔,吴玉薇,傅广礼 84true 王大成 83true 王大成,梁栋材 82true 王大成,梁栋材 81true 王大成,梁栋材 80true 傅亚珍 79true 傅亚珍 78true 李文杰 77true 李文杰 76true 李安之,刘键 75true 赵保路 74true 聂玉生 73true 丰玉璧 72true 何润根 71true 季静秋,冯晋臣 70true 赵保路 69true 张志鸿,刘宏志 68true 张志鸿,刘宏志 67true 张志鸿,刘宏志 66true 苏兆众,张青青 65true 胡坤生 64true 秦生巨,李莉 63true 张敬礼,陈泮藻 62true 赵保路 61true 郭爱克,杨先一 60true 郭爱克,杨先一 59true 陈枢青 58true 赵敏 57true 郭尧君 56true 韩玲,陈杞 55true 田心棣,Z.SALAMON,V.KOCHEV,A.OTTOVA,M.ZVIMAN 54true 刘妙良 53true -19g/μm³)使得生物样品含有极低浓度甚至是痕迹量的元素分析成为可能。目前,二次离子显微镜已广泛应用于细胞生物学、核医学、植物生理学和人类病理学等领域。]]> 徐学红 52true 江丕栋 51true SHEN Ping 50true SHEN Ping 49true E.coli and the formation of transduction complex. BIA also been used wildly in molecular biology research to study the interaction between transcription factor and its operon (DNA).]]> SHEN Ping 48true SHEN Ping 47true SHEN Ping 46true ZHANG Zhong-Lun 45true ZHANG Zhong-Lun 44true HE Zhi-Wei and YAO Kai-Tai 43true ZHANG Zhong-Lun 42true SUI Jian-Hua and SONG Zeng-Xuan 41true ZHANG Zhong-Lun,YANG Feng-Zhen and ZHANG Xin-Rong 40true WANG Hua-Chun and CHEN Qing-Xuan 39true YANG Bing-Hu and WANG Sheng-Qi 38true P＜0.05). The urine luminescence intensity of bone tumor patients significantly decreased after operation (P＜0.05). The luminescence from blood and different organs of nude mice were measured. The results showed that the luminescence intensities from different organs increased greatly after inoculation.]]> ZHANG Zhong-Lun 37true ZHANG Zhong-Lun 36true ZHANG Zhong-Lun 35true WU Hui-Ling and ZOU Qiang 34true WANG Jian-Long 33true YU Wei,SUN Yong-Kang,GU Ning and WU Jian-Guo 32true YANG Chun-Mei,WAN Bai-Kun and GAO Xiao-Feng 31true in vivo are briefly reviewed.]]> WANG Jin-Jun,CHEN Xiao-Chuan and XING Da 30true C.elegans and Drosophila. Initial attempts to activate the RNAi pathway in mammalian cells were unsuccessful, since the introduction of dsRNA＞30 nucleotides in length results in non-specific suppression of gene expression. Much of this response is due to activation of the dsRNA-dependent protein kinase PKR, and the subsequent phosphorylation and inactivation of the translation factor elF2a. As RNAi mechanism being extensively studied, researchers discovered that double stranded short interfering RNA (siRNA) oligotides of 21～23 nucleotides could be used to mediate a gene silencing effect in mammalian cells. The application of RNAi to mammalian cells has the potential to revolutionize the field of functional genomics. The ability to simply, effectively, and specifically down-regulate the expression of genes in mammalian cells holds enormous scientific, commercial, and therapeutic potential.]]> CHEN Jie,BAI Chun-Xue and ZHANG Min 29true WANG Yong-Yu and ZHANG You-Yi 28true ZHANG Cheng-Gang and HE Fu-Chu 27true in vivo and the knowledge gathered from such methods will decipher the protein complexes network. Now, it is also a powerful tool available for proteomics research. This new technology will continue to be developed as it is now became extraordinary valuable to study protein-protein interaction.]]> CHENG Yong-Sheng and LIU Jin-Yuan 26true MENG Lei and SONG Zeng-Xuan 25true WU Hao-Yang and WU Yu-Yuan 24true 2006/11/17 0:00:00 WANG Wei-Ping,WANG Zhi-Chang,ZHANG Xie,LAI Jin-Hu and CHEN Huan-Wen 23true 2006/10/18 0:00:00 XU Yao-Gang,PANG Xiao-Feng and JI Juan-Juan 22true 2007/12/11 0:00:00 SUN Zi-Cai,WEI Zheng and WEI Ke-Mei 21true Caenorhabditis elegans is one kind of the most important modal animals for studying heredity, development and behaviors, and for investigating the mechanisms underlying from molecular to systematic levels. Gene microinjection and integration are the most important ways to make transgenic animals in C. elegans. They are widely used in the studies of expression, function and interaction of genes. Briefly, a DNA construct (plasmid or cosmid) or PCR product which carried gene(s) of interest is mixed with a co-injection marker and injected into the distal gonad (syncytium) through a micropipette. The DNA injected is taken up into the mature oocytes and exists as an unintegrated extrachromosomal array, which segregates randomly and can be lost. The extrachromosomal arrays are integrated optionally into chromosomes by irradiation of γ rays, X rays, or by TMP/UV integration method. Here, the detail processes and key points of microinjection and gene integration in C. elegans was introduced and the know-how in these techniques was offered.]]> 2008/11/10 0:00:00 JIU Ya-Ming,ZHANG Rong-Ying and WU Zheng-Xing 20true z axis, and their combinations with the methods to increase xy plane resolution mentioned above are explained. In the end, the limitation to the current super-resolution methods and their future direction are also discussed.]]> 2009/9/7 0:00:00 LV Zhi-Jian,LU Jing-Ze,WU Ya-Qiong and CHEN Liang-Yi 19true 2009/6/19 0:00:00 XIE Hao,YANG Cheng and CHEN Li-E 18true 2009/10/12 0:00:00 MI Wei and WANG Jing 17true 2009/9/7 0:00:00 SUN Rui-Xiang,DONG Meng-Qiu,CHI Hao,YANG Bing,XIU Li-Yun,WANG Le-Heng,FU Yan and HE Si-Min 16true 2009/9/7 0:00:00 LI Chang-Yan,LI Qian,LIU Hai-Tao,ZHANG Jun and DAMIRIN Aletangaole 15true 2010/9/14 0:00:00 ZHANG Xian-Fang,LIU Jun,GUO Yun-Zhu,MA Xiao-Liang and YIN Da-Chuan 14true 2010/10/12 0:00:00 XIN Wen-Wen and WANG Jing-Lin 13true 2011/9/30 0:00:00 LI Ming,JIANG Jie,LI Hong-Mei and XU Rui-Feng 12true 2013/3/26 0:00:00 CHANG Cheng,WU Song-Feng,MA Jie,ZHANG Wei and ZHU Yun-Ping 11true 2012/3/19 0:00:00 LI Liang,SUI Zhi-Wei,WANG Jing,ZANG Chao and YU Xiao-Bo 10true 2013/3/22 0:00:00 WU Peng,HE Fu-Chu and JIANG Ying 9true i.e., some digested peptides (precursor ions) are chosen to fragment and produce tandem mass spectra, which can be identified by database search, and then proteins can be inferred from the identified peptides. In the identification, precursor mass is a key parameter. Whether the correct peptide is within the peptide candidates depends on whether the precursor mass is the monoisotopic mass. The number of peptide candidates depends on the accuracy of the precursor mass. This research investigates the accurate determination of precursors in terms of monoisotopic peak determination and systematic error elimination. There are some techniques of monoisotopic peak determination on protein level, including charge state determination, monoisotopic peak determination and overlapping cluster determination. Some of them can be used to the monoisotopic peak determination of precursors. Meanwhile, there are some well-known methods for systematic error elimination. The two kinds of techniques for accurate precursor determination can help increase the large-scale protein identification rate.]]> 2013/2/28 11:03:21 YUAN Zuo-Fei,WU Long,LIU Chao,CHI Hao,FAN Sheng-Bo,ZHANG Kun,ZENG Wen-Feng,SUN Rui-Xiang and HE Si-Min 8true 2014/11/21 0:00:00 ZHANG Hai-chuan,LI Jie and WANG Ya-Dong 7true 2016/9/18 0:00:00 LIU Hang, YAO Jun, YANG Peng-Yuan and ZHANG Yang 6true 2016/2/19 0:00:00 CHENG Wei, LI Mei, DU Sha and ZHENG Jie 5true 2016/11/21 0:00:00 DONG Sheng-Hua, ZHANG Jing and GE Sheng-Xiang 4true 2018/4/8 0:00:00 HUANG Chuan-Xi, MA Jie, XU Kai-Kun, WU Chen and ZHU Yun-Ping 3true 2019/9/25 0:00:00 YANG Chuan-Hui,LU Rong-Rui and YANG Yu-Feng 2true 2024/12/2 12:08:36 ZHAO Jia-Min,LU Cheng-Jiang,YANG Ming,Buhe Nashun and WANG Gang 1true