佛波酯诱导大鼠滑膜细胞骨架改变的研究
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国家重点基础研究发展计划(2007CB307002), 国际科技合作重点项目计划(2005DFA10170)和高等学校学科创新引智计划(B07013)资助项目.


Study of Cytoskeleton Change in Rat Synoviocytes Induced by PMA
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This work was supported by grants from National Basic Research Program of China (2007CB307002), Key International S&T Cooperation Project of China (2005DFA10170) and the Programme of Introducing Talents of Displine to Universities (B07013).

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    摘要:

    以体外培养的大鼠关节炎模型的滑膜细胞为研究对象,用蛋白激酶C(PKC)激活剂佛波酯(phorbol 12-myristate 13-acetate, PMA)刺激细胞模拟炎症过程,应用原子力显微成像技术研究了大鼠滑膜细胞在PKC活化后细胞骨架变化情况.结果表明,利用原子力显微镜成像可以较好地表征PMA刺激引起滑膜细胞骨架的收缩隆起及细胞表面质地的变化,并揭示了PKC活化可能是引起滑膜细胞骨架改变的重要原因.这些结果为加深了解类风湿关节炎的发生机制提供了实验基础.

    Abstract:

    Rat synoviocytes were cultured in vitro and the changing of cellular cytoskeleton induced by PMA was investigated with atomic force microscopy. PMA is usually used to stimulate PKC signal transduction pathway in synoviocytes and to simulate process of inflammation. The results presented here show that synoviocyte cytoskeleton has changed significantly after PKC activation by using the imaging of atomic force microscopy. The results also indicate that the activation of PKC might play crucial role in cytoskeleton changing of synoviocytes,which, to some extent, provides some experiments and ideas on understanding the mechanism underlying rheumatoid arthritis (RA).

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潘雷霆,孙文武,齐继伟,吴限,宋昆,胡芬,张心正,许京军.佛波酯诱导大鼠滑膜细胞骨架改变的研究[J].生物化学与生物物理进展,2009,36(1):83-87

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历史
  • 收稿日期:2008-05-08
  • 最后修改日期:2008-07-22
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  • 在线发布日期: 2008-08-19
  • 出版日期: 2009-01-20