2012年第39卷第12期目录

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封面故事:BMP9是目前已知的BMPs中诱导MSCs(mesenchymal stem cells,MSCs)成骨分化能力最强的一种,具有广阔的潜在临床应用价值,但BMP9调控MSCs成骨分化的信号转导体系及其调控机理却有待阐明.罗进勇等的研究发现:在MSCs中BMP9能活化ERK1/2激酶途径;当利用抑制剂抑制ERK1/2后,BMP9诱导的MSCs的早期和晚期成骨分化进一步增强,而RNA干扰导致ERK1/2基因沉默同样也可进一步促进BMP9诱导的MSCs体外成骨分化,并促进BMP9诱导的MSCs在裸鼠皮下异位成骨.因此BMP9可通过活化ERK1/2激酶从而调控MSCs成骨分化,而且其调控机制可能与影响成骨关键转录因子Runx2和Smad经典途径有关.上述结果将进一步丰富目前了解甚少的BMP9诱导和调控MSCs成骨分化的机理,为其今后在临床的应用积累实验数据. (宋 涛,何娟文,王 锦,唐 敏,罗进勇. 阻断ERK1/2激酶可增强骨形态发生蛋白9诱导的间充质干细胞成骨分化,本期第1197~1206页)

Cover Story:In our previous reports, BMP9 has shown potent function to induce osteogenic differentiation of mesenchymal stem cells, however, the underlying molecular mechanism of BMP9-induced osteogenesis is needed to be deep explored. In this study, BMP9 was introduced into mesenchymal stem cells by recombinant adenoviruses protocol, then, in vitro and in vivo assays were conducted to evidence whether BMP9 can regulate osteogenic differentiation of mesenchymal stem cells through ERK1/2 kinase pathway. The results showed that BMP9 can activate ERK1/2 kinase through increase the phosphorylated form of ERK1/2 kinase. ERK1/2 kinase inhibitor PD98059 can increase the ALP activity, OPN expression and calcium deposition of C3H10T1/2 cells induced by BMP9. Furthermore, PD98059 also led to enhancement of Runx2 activity and activation of canonical Smad pathway stimulated by BMP9. When ERK1/2 kinase was silenced by RNA interference, BMP9-activated Smad pathway was further enhanced. Moreover, ALP activity, calcium deposition and in invo ectopic bone formation were accordingly increased along with knockdown of ERK1/2 kinase. Taken together, those results intensively suggested that BMP9 can activate ERK1/2 kinase, and inhibition of ERK1/2 kinase can enhance BMP9-induced osteogenic differentiation of mesenchymal stem cells. ERK1/2 are highly capable of negatively regulate BMP9-induced osteoblastic differentiation of mesenchymal stem cells.

综述与专论

microRNAs与TP53基因调控网络研究进展龚朝建,黄宏斌,徐 柯,梁 芳,李小玲,熊 炜,曾朝阳,李桂源  [摘要][PDF][HTML]

高密度脂蛋白胆固醇水平相关的遗传学研究最新进展刘晓艳,路倩,陈五军,唐朝克  [摘要][PDF][HTML]

基于RNA-Seq的长非编码RNA预测孙磊,张林,刘辉  [摘要][PDF][HTML]

统计学习的认知机制及其神经基础武秋艳,邓园  [摘要][PDF][HTML]

研究报告

初级视皮层神经元对瞬态刺激响应的时间性质李骁健,蒋震,王毅  [摘要][PDF][HTML]

阻断ERK1/2激酶可增强骨形态发生蛋白9诱导的间充质干细胞成骨分化宋涛,何娟文,王锦,唐敏,罗进勇  [摘要][PDF][HTML]

γ-氨基丁酸信号对小鼠胎盘早中期建立的影响罗文萍,谭冬梅,杨根岭,卢俊杰,赵 海,谭 毅  [摘要][PDF][HTML]

幽门螺旋杆菌肿瘤坏死因子α诱导蛋白活性分子的构建、结晶及初步晶体学研究高明明,张英,王大成  [摘要][PDF][HTML]

亮氨酸拉链提高小鼠血浆中剪接的凝血因子Ⅷ凝血活性朱甫祥,刘泽隆,缪 静,屈慧鸽,迟晓艳  [摘要][PDF][HTML]

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