2013年第40卷第3期目录
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封面故事:放射性肠损伤常见于核事故及战时的核爆炸,也是临床腹部肿瘤放疗过程中最为常见的并发症之一.近年来,间充质干细胞(mesenchymal stem cells,MSCs)以其独特的生物学特性而备受科学家的青睐,它具有分泌细胞因子、重建造血微环境、低免疫原性、易于外源基因的转染和表达等优点,将其应用于放射性肠损伤的临床治疗具有十分广阔的前景.杨超等以GFP为示踪蛋白,将人MSCs移植到放射性肠损伤的NOD/SCID小鼠体内,通过免疫荧光法检测发现MSCs可定植于受损的小肠组织,并表达波形蛋白(vimentin)和α-SMA,提示其可向间质细胞分化.研究结果为放射性肠损伤的临床治疗提供了新的思路.
(杨 超 张晓梅 宫 媛 王昌正 韩 颖 吴本俨. 间充质干细胞移植对放射性肠损伤修复作用的实验研究,本期第249~254页)
Cover Story:The study was aimed to investigate the effects of human bone marrow mesenchymal stem cells (MSCs) in the radiation-induced intestinal injury. MSCs were expanded, labeled with enhance green fluorescentprotein (eGFP). The NOD/SCID mice were randomly assigned to one of three groups: (A) without radiation; (B) and (C) received abdominal radiation with 5 Gy 60Co γ-rays at the rate of 100 cGy/min. (B) i.v. transplanted with physiological saline, (C) i.v. transplanted with MSCs. Mice were weighed daily and observed for 15 days after irradiation. Intestines of mice were obtained in order to assay histological changes by hematoxylin and eosin staining. The immunofluorescence staining showed that the transplanted cells could differentiate into vimentin /α-SMA fibroblastic-like cells in vivo. These data indicated that MSCs could engraft at the injured small intestine, and contribute to the repairment of irradiated intestine. This could provide a valuable strategy for radiation-induced enteritis.
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综述与专论
研究快报
研究报告
技术与方法
新技术讲座
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