2014年第41卷第4期目录
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封面故事:基因调控网络(gene regulatory network)是指细胞内(或特定一个基因组内)基因和基因之间的相互作用关系所形成的网络.恶性肿瘤发生发展过程中存在基因调控网络紊乱.构建基于芯片数据的基因调控网络,找到最能反映基因之间相互关系的网络表达特征,具有十分重要的生物学意义.RASSF1A基因是在恶性黑色素瘤组织中表达降低的抑瘤基因.基因芯片研究发现,RASSF1A影响了A375细胞周期、细胞死亡、细胞运动等相关基因表达,这些差异表达基因之间存在广泛而复杂的相互作用,构成一个高连接度的基因网络,炎症细胞因子IL-6、转录因子C-Jun、c-FOS作为“网络节点”,彼此间通过复杂的相互作用形成多维的和动态的“互联网”,调节细胞生物行为的走向.
(易 梅,李 吉,粟 娟,张江林,陈 翔,杜乾君,向 波,熊 炜,李小玲,谢红付. RASSF1A对黑色素瘤A375细胞基因网络的影响,本期第352~361页)
Cover Story:Gene regulatory networks have an important role in every process of life, including cell differentiation, metabolism, the cell cycle and signal transduction. The RASSF1A is a tumor suppressor gene involved in several growth regulatory and pro-apoptotic pathways. In this study, we have used microarray technology to define differences in the gene expression profiles subsequent to exogenous wild-type RASSF1A in melanoma A375 cells. Gene expression changes were verified in a subset of genes using real time RT-PCR. Association of modulated genes with biological functional groups identified several pathways affected by RASSF1A including cell death, cellular growth and proliferation, and cellular development. GRN of modulated genes were identified using the STRING. Pro-inflammatory factors and transcription factors locates in the center of RASSF1A modulated gene regulatory networks. Our results suggested that RASSF1A might affect melanoma gene regulatory network via modulating the expression and interaction between pro-inflammatory factors and transcription factors.
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综述与专论
研究报告
技术与方法
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