2015年第42卷第1期目录
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封面故事:侵袭与转移是鼻咽癌患者复发与预后差的最主要原因.鼻咽癌的侵袭和转移与EB病毒感染有关.EB病毒潜伏性膜蛋白1是公认的病毒基因组编码、具有促细胞癌变和转移作用的瘤蛋白,该蛋白3个羧基端活性区通过与细胞内不同蛋白质分子的结合,启动复杂的细胞内信号通路,使细胞呈现相应的生物学功能.肿瘤组织中数量极少的肿瘤干细胞具有顽强的生存能力,决定肿瘤发生、发展和转移,被认为是肿瘤发生和复发的根源.肖娟,张志伟等的研究结果展示了潜伏性膜蛋白1第三个功能活性区在鼻咽癌干细胞内调控的已知或未知基因,为进一步揭示鼻咽癌细胞内复杂的信号网络提供了素材,也为阐明鼻咽癌侵袭与转移分子机制提供新的实验与理论依据.
(肖 娟,张志伟,伍石华,刘重元,唐 薇,赵 强,雷明生,杨代水. LMP1羧基端活化区3对鼻咽癌干细胞SP18迁移与侵袭的影响,本期第65~72页)
Cover Story:To investigate mechanism of migration and invasion effected by carboxyl terminal activating region 3 (CTAR3) of Epstein-Barr virus encoded latent membrane protein 1(LMP1) in nasopharyngeal carcinoma stem cell SP18 cells, the SP18 cell of stable expressed LMP1 and deletion mutant type LMP1 (LMP1△232-351) were established (SP18-LMP1 and SP18-LMP1△252-351). The effect of LMP1-CTAR3 deletion mutant for cellular proliferation, migration and invasion were observed in SP18 cells. The differential expression genes between SP18-LMP1 and SP18-LMP1△252-351 cells were analyzed by cDNA chips, and the expression levels of partial identified genes were verified by fluorescent Real-time quantitative RT-PCR. The relation of differential expression genes were analyzed by bioinformatics. The results showed: (1) The ability of LMP1△232-351 promoting SP18 cell proliferation, migration and invasion was obviously decreased to compare with wild type LMP1(n=3, P < 0.05). (2) 18 genes, 13 up- and 5 down-regulated ones, of LMP1-CTAR3 mediated regulation with migration and invasion were identified from in SP18 cell lines. The differential expression of partial identified genes was similar with cDNA chips separated ones and confirmed by fluorescent Real-time quantitative RT-PCR. (3) 13 differential expression genes can be cross-talk, and among FN1, MMP14, ITGA2, THBS1, IL1B and IL6 genes were frequently correlation. These results suggested that LMP1-CTAR3 probably regulates the expression of FN1, MMP14, ITGA2, THBS1, IL1B and IL6 genes to induce and promote migration and invasion of nasopharyngeal carcinoma stem cell SP18 cell.
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综述与专论
研究快报
研究报告
技术与方法
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