2016年第43卷第5期目录
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封面故事:癌症干细胞具有高转移、耐药性和自我更新能力,是癌症病人死亡率高的根本原因.王毅刚研究组利用悬浮培养富集的类肝癌干细胞在自我更新和分化能力、干细胞相关转录因子表达(如NANOG和 OCT4)、静息状态和耐药性等方面具有癌症干细胞的特性.已有研究表明,溶瘤腺病毒ZD55能够靶向肝癌并且表现出明显的细胞毒性效应,然而,其对肝癌干细胞是否具有同样的杀伤效力仍需进一步探讨.我们的实验结果表明,悬浮培养的Huh7类肝癌干细胞经溶瘤腺病毒ZD55处理后表现出明显的细胞毒性效应和杀伤特性,类肝癌干细胞的最低生存率仅为26.7%,而且ZD55能够非常明显地诱导类肝癌干细胞凋亡,其凋亡率最高达到60%.因此,ZD55可能会成为靶向肝癌干细胞的一种很有前景的治疗药物,对肝癌的临床治疗具有一定的应用价值.
(张新敏,张 蓉,孟树林,谢文杰,王毅刚.溶瘤腺病毒ZD55对类肝癌干细胞的抑制效应,本期第496~505页)
Cover Story:Oncolytic adenovirus could target and kill cancer stem cells and recognized as a promising anticancer agent. Our previous studies showed that oncolytic adenovirus ZD55 was developed to target liver cancer and exhibited obvious cytotoxicity effect. However, it still remained to be confirmed that whether ZD55 could also effectively eliminate liver cancer stem cells. We first utilized the suspension culture to enrich the liver cancer stem-like cells and validate the properties of acquired liver cancer stem cells. Further, we use MTT, Hoechst staining, Western blot, flow cytometry assay to detect cell viability, apoptotic effect and cytopathic effect in liver cancer stem-like cells after treatment with ZD55. The results indicated that liver cancer stem-like cells had the features of self-renewal, differentiation, high expression of cancer stem cell-related transcription factors (eg.NANOG and OCT4), quiescence, chemo-resistance. Oncolytic virus ZD55 resulted in obvious cytotoxicity and killing effect (the minimum cell viability for Huh7 sphere is 26.7%) on liver cancer stem-like cells, and induced significant cell apoptosis (the maximum apoptosis rate for Huh7 sphere is 60%). Thus, ZD55 might virtually represent an attractive therapeutic agent for targeting liver cancer stem cells to achieve better clinical outcome for HCC patients.
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综述与专论
研究报告
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