2016年第43卷第9期目录
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封面故事:抗体通过与抗原发生特异性的结合抵御外来入侵物.因而其研究具有重要的医学应用价值.蛋白质的结构柔性和结构动态特征常常被忽略.传统的蛋白质结构检测手段通常提供单一的静态的三维结构.为此,我们通过优化样品制备方法和开发单个分子的电子显微镜断层扫描三维重构技术,提出了IPET方法,并且用IPET方法研究具有极强的结构柔性和多样性的生物大分子.以抗体作为例子,通过对大量不同分子的断层扫描图像的收集与三维重构,统计分析其构象,可以描述蛋白质的结构柔性及动态结构的空间特征.IPET方法的核心是断层扫描图像的精确对齐和三维反投影,并不依赖初始模型,也不受限于样品制备方法的选择,对于冷冻方法制备的样品同样适用.因此该方法有望广泛应用于柔性蛋白质三维结构的研究.
(张 星,廖雨恒,童慧敏,张 磊,张胜利,任 罡.使用电子断层技术对IgG1抗体逐个分子的三维重构与结构分析,本期第839~849页)
Cover Story:Antibody (also named as an immunoglobulin, Ig), a most important macromolecule for immune response in human body, has been developed as macromolecular drug to treat the cancer and immune diseases. Understanding of antibody three-dimensional (3D) structure and fluctuation could be an important step for further understanding and controlling the antibody pharmacological function. However, the study is limited by antibody flexible structure. In this paper, we reviewed the current research progresses on structural study of human IgG1 antibody conducted by our recently developed individual-particle electron tomography (IPET) method. The review includes the sample preparation method, basic logic of image processing strategy, 3D analysis and its application in antibody-drug conjunction and antibody structural fluctuation. We also discussed the strengths and weakness of the technique.
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综述与专论
研究报告
新技术讲座
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