2017年第44卷第7期目录

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封面故事:朱艳霞研究组通过仙台病毒将椎间盘退变患者髓核细胞重编程为诱导多能干细胞(iPSC),重编程后的iPSC可能保留了部分髓核特性的记忆,从而使髓核来源的iPSC相对于成纤维细胞来源的iPSC而言更易于向髓核细胞分化,所建立的三维水凝胶体系可促进iPSC向髓核样细胞分化.髓核来源iPSC和髓核细胞分化体系的建立,不仅为椎间盘退变发病机制的研究提供较好的细胞模型,也可为椎间盘退变临床细胞治疗提供一个较好细胞来源.
(吴张松,罗志强,张明裕,廉翠红,林欣燕,梁雨虹,顾洪生,朱艳霞.人椎间盘髓核来源的诱导多能干细胞重编程及功能的研究,本期第591~600页)

Cover Story:Intervertebral disc degeneration is believed to originate in the nucleus pulposus (NP) region, it is important to obtain greater numbers of active NP cells for the study and therapy of disc degenerative disease (DDD). Human induced pluripotent stem cells (iPSCs) are a powerful tool for modeling human development and disease, as well as for their potential applications in regenerative medicine. We isolated NP cells from DDD patients with our improved method, and reprogramed primary NP cells into iPSCs with Sendai virus vectors encoding 4 factors. Successful reprogramming of iPSCs was verified by specific surface markers and teratoma, and differentiation of iPSCs into NP-like cells was performed in culture plate and hydrogel, with skin fibroblast derived-iPSC used as control. It was demonstrated that iPSCs derived from NP cells were featured with normal karyotype, and showed expression of pluripotency markers and were able to form teratoma in nude mice. NP induction of iPSCs resulted in their expression of NP cell specific matrix proteins and related genes. NP derived-iPSCs without induction also showed a basal level of expression of some NP-like phenotype characteristics. What’s more, NP derived-iPSCs prefer to differentiate into NP-like cells in hydrogel rather than in culture plate.This is the first protocol for generation of iPSCs from NP cells of DDD patients, and we successfully differentiated this iPSCs into NP-like cells in hydrogel. This method opens a window in the treatment of DDD by using patient-specific NP cells in a relatively simple and straight forward manner.

综述与专论

RNA翻译的复杂性:不翻译、部分翻译、从头翻译及过度翻译郝亚静,骆健俊,张宝,陈润生  [摘要][PDF][HTML]

长链非编码RNA的免疫调节机制研究进展夏 莉,周玉峰  [摘要][PDF][HTML]

tRNA衍生片段和tRNA半分子的生物学功能及其在疾病发生中的作用朱林文,谢 依,郭俊明  [摘要][PDF][HTML]

C2H2型锌指蛋白结合的DNA序列预测方法的研究进展沈磐,杨冬,贺福初  [摘要][PDF][HTML]

研究报告

LncRNA-GAS5抑制miR-21介导的非完全匹配靶mRNA降解郑伟,董洁,李少华,丁红梅,李慧,黄皑雪,夏伟,白琛俊,郭晓华,李达,耿介,李洁,邵宁生  [摘要][PDF][HTML]

人椎间盘髓核来源的诱导多能干细胞重编程及功能的研究吴张松,罗志强,张明裕,廉翠红,林欣燕,梁雨虹,顾洪生,朱艳霞  [摘要][PDF][HTML]

病理浓度甲醛的累积导致小鼠神经母瘤细胞活力及黏附能力下降王腈,周均,莫炜川,赫英舸,魏艳,赫荣乔,易发平  [摘要][PDF][HTML]

视知觉学习对于识别动态随机点立体图深度运动方向的作用张地,Vincent Nourrit,桑新柱,Jean-Louis de Bougrenet de la Tocnaye  [摘要][PDF][HTML]

Letter to Editor

Nonlabens Dokdonensis视紫红质2中的钠离子结合与pH依赖性的传递赵宏深,陈德亮  [摘要][PDF][HTML]

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