2018年第45卷第12期目录
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封面故事:钴(Co)是植物生长所需的微量元素之一,是维生素B12的组成部分.低浓度的钴对植物生长有较好的促进作用,但当土壤中钴浓度达到10 mg/L时,对植物的生长发育有抑制作用,甚至导致死亡.过量Co还会通过食物链的传递对人体健康造成极大危害.OsPDR是一个镉响应的金属离子转运蛋白.通过对从水稻(Oryza sativa cv. Nipponbare)中分离的OsPDR基因进行金属离子转移活性分析发现,过表达OsPDR能提高酵母对Co的耐受性,但对Zn、Ni和Cd的耐受性不强,过表达OsPDR的酵母中Co的积累较空载体转化酵母中Co的积累更高.利用共聚焦显微镜观察发现,EGFP-OsPDR融合蛋白定位于液泡膜上.这些数据表明OsPDR在酵母中对Co稳态起着重要作用.
(田思琪,乔 坤,王凡红,梁 爽,王 红,柴团耀.OsPDR在酵母中异源表达增强了酵母对钴的耐受性,本期第1259~1267页)
Cover Story:A cadmium-responsive metal ion transporter OsPDR, which was highly upregulated in the shoot of rice (Oryza sativa ssp. japonica cv. Nipponbare) at 24 h after treatment with 50 μmol/L Cd, was identified in cadmium-responsive transcriptome profiles analyzed by RNA-Seq. In this study, we isolated OsPDR from rice (Oryza sativa cv. Nipponbare) and the metal ions transport activity of OsPDR was analyzed. The results of metal tolerance experiments showed that overexpression of OsPDR can enhance yeast’s tolerance to Co, but not to Zn, Ni or Cd, and higher accumulation of Co was observed compared to the empty vector-transformed yeast as determined by inductively coupled plasma mass spectrometry (ICP-MS). The EGFP-OsPDR fusion protein was localized to the vacuolar membrane using confocal microscopy. These data suggested that OsPDR might play an important role in Co homeostasis. Additional studies are needed to understand the function of OsPDR in plants.
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综述与专论
研究报告
技术与方法
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