陈欣菊.长链非编码RNA在消化系统肿瘤发生中的作用[J].生物化学与生物物理进展(待发表)
长链非编码RNA在消化系统肿瘤发生中的作用
LncRNA MIR31HG Inhibits the Proliferation of Esophageal Squamous Cell Carcinoma Cells by Inducing Cell Cycle Arrest
投稿时间:2017-12-11  修订日期:2018-05-16
中文关键词:  LncRNA  MIR31HG  食管鳞癌  细胞增殖  细胞周期
英文关键词:LncRNA  MIR31HG  Esophagus carcinoma  Cell proliferation  Cell cycle
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作者单位E-mail
陈欣菊 the first affiliated hospital of henan university of TCM xinj_chen@163.com 
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中文摘要:
      本研究探讨lncRNA MIR31HG对食管鳞癌细胞增殖活性的影响. 利用定量PCR检测MIR31HG在食管鳞癌标本及其癌旁组织、人食管上皮细胞系Het-1A和食管鳞癌细胞系Eca-109、EC-1、KYSE30中的表达. 采用过表达质粒pcDNA3.1-MIR31HG在食管鳞癌细胞系中过表达MIR31HG,MTT法和SRB法检测细胞增殖率,细胞周期分析试剂盒检测细胞周期进程,Caspase3活性检测试剂盒分析Caspase3活性,PCR和Western blot 法检测 p53、Caspase3及 Bcl-2 的mRNA和蛋白表达水平. 结果显示,食管癌组织中MIR31HG表达水平显著低于癌旁组织(P<0.05); 与Het-1A细胞相比,Eca-109、EC-1、KYSE30细胞中MIR31HG的表达均显著下调(P<0.05),提示MIR31HG可能介导食管癌的发生发展. 转染pcDNA3.1-MIR31HG可显著上调食管癌细胞中MIR31HG的mRNA表达(P<0.01),且MIR31HG过表达可显著抑制食管癌细胞增殖活性(P<0.05),减少S期细胞数(P<0.05),增加G1期细胞数(P<0.05),提示MIR31HG可能通过阻碍细胞周期G1期-S期进程抑制食管癌细胞增殖活性. 此外,MIR31HG过表达显著增加Caspase3活性,增加Caspase3和p53的mRNA和蛋白表达水平,同时抑制Bcl-2 mRNA和蛋白表达水平. 这表明,MIR31HG可通过抑制食管癌细胞的增殖活性阻碍食管癌的发生发展,这可能为食管癌的诊断和治疗提供新策略.
英文摘要:
      The aim of present study is to investigate the effects and the mechanism of lncRNA MIR31HG on the proliferation of esophageal squamous cell carcinoma (ESCC) cells. The mRNA levels of MIR31HG were tested by qPCR in specimens of esophageal cancer and para-carcinoma tissues, as well as in esophageal epithelial cell line Het-1A and in ESCC cell lines Eca-109, EC-1 and KYSE30. MIR31HG was overexpressed in Eca-109, EC-1 and KYSE30 cells by using overexpression plasmid pcDNA3.1-MIR31HG. Cell proliferation was then tested by both MTT and SRB methods. Cell cycle progression was detected by using Cell Cycle Assay Kit, and the activity of Caspase3 in cells was tesed by Caspase3 Activity Assay Kit. The mRNA and protein levels of p53, Caspase3 and BCL-2 were measured by qPCR and Western blot. The expression of MIR31HG was significantly decreased in esophagus cancer comparing with para-carcinoma tissues, as well as in Eca-109, EC-1, KYSE30 cells comparing with Het-1A (P<0.05). Transfection of pcDNA3.1-MIR31HG significantly increased MIR31HG expression in ESCC cell lines(P<0.01), inhibited cell viability, reduced S-phase cells numbers and increased the G1-phase cells numbers (P<0.05). These results suggested that MIR31HG may reduce cell viability of ESCC cells though impeding cell cycle. Moreover, MIR31HG overexpression also significantly increased Caspase3 activity, Caspase3 and p53 expression and decreased Bcl-2 expression in ESCC cells (P<0.05). Taken together, these results suggest that MIR31HG may hinder the development of esophageal cancer by inhibiting the proliferation of ESCC cells, which may provide a new strategy for the diagnosis and treatment of esophageal cancer.
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