尚晓娟,彭洪尚.基于超顺磁性Fe3O4纳米粒子调控3D肿瘤细胞球生长的研究[J].生物化学与生物物理进展(待发表)
基于超顺磁性Fe3O4纳米粒子调控3D肿瘤细胞球生长的研究
Based on superparamagnetic Fe3O4 nanoparticles to regulate the growth of 3D tumor cell spheres
投稿时间:2019-04-14  修订日期:2019-06-20
中文关键词:  3D肿瘤球  Fe3O4 纳米粒子  表面修饰  磁场
英文关键词:3D tumor sphere  Fe3O4 nanoparticle  Surface modification  Magnetic field
基金项目:国家自然科学基金项目(61775245)
作者单位E-mail
尚晓娟 中央民族大学 15737926823@163.com 
彭洪尚 中央民族大学 E-mail: hshpeng@muc.edu.cn 
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中文摘要:
      肿瘤球体外培养近年来在生物医学研究中有着越来越广泛的应用,但同时也存在形貌不完整,培养周期长,大小不一等问题。为了提高肿瘤球培养的质量,本文对肿瘤球培养方法进行了改进。首先对Fe3O4纳米颗粒进行氨基化修饰以提高其生物亲和性,而后与肿瘤细胞共培育以负载Fe3O4纳米颗粒,利用外加磁场来调控肿瘤球生长并探究对肿瘤球生长的影响。通过透射电镜、动态光散射、X射线衍射等分析,氨基化的超顺磁性Fe3O4纳米粒子形貌为球形或类球形,分布均匀,直径约为10-16 nm,修饰前后晶体结构不变为立方结构,Zeta电位为正值。MTT法检测表面Fe3O4纳米粒子具有良好的生物兼容性,且在一定范围内对细胞活性具有正面作用。通过普鲁士蓝染色和核磁共振成像证明,氨基化的Fe3O4纳米粒子可以被肿瘤细胞高效吞噬。改变吞噬Fe3O4纳米粒子的浓度和外加磁场强度,对所培育的肿瘤球形貌及大小进行了研究,发现外磁场可以在一定程度上对瘤球的生长进行调控。
英文摘要:
      In recent years, in vitro culture of tumor pellets has been widely used in biomedical research.In order to improve the problems existing in the in vitro culture of tumor pellets, the method of tumor pellet culture was improved. Based on the fact that tumor cells devoured Fe3O4 nanoparticles, an external magnetic field was proposed to regulate the growth of 3D tumor pellets.First, Fe3O4 nanoparticles were modified by amination to prepare hydrophilic superparamagnetic Fe3O4 nanoparticles. Then, tumor cells were allowed to engulf the nanoparticles. Finally, a magnetic field was applied to regulate tumor ball growth and investigate the effect of magnetic field on tumor cells.The experimental results showed that the morphology of superparamagnetic Fe3O4 nanoparticles prepared by amination modification was spherical or quasi-spherical, with uniform distribution and about 10-16nm in diameter. The crystal structure did not change into a cubic structure before and after modification, and Zeta potential was positive.MTT assay showed no toxicity to cells, and within a certain range, with the increase of the concentration of nanoparticles, the amount of phagocytic cells increased, and the external magnetic field had a certain effect on the morphology and size of tumor spheres.It indicates that the external magnetic field has certain regulatory effect on tumor ball growth of Fe3O4 magnetized nanoparticles.
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