Vol.39,No.12,2012

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Cover Story:In our previous reports, BMP9 has shown potent function to induce osteogenic differentiation of mesenchymal stem cells, however, the underlying molecular mechanism of BMP9-induced osteogenesis is needed to be deep explored. In this study, BMP9 was introduced into mesenchymal stem cells by recombinant adenoviruses protocol, then, in vitro and in vivo assays were conducted to evidence whether BMP9 can regulate osteogenic differentiation of mesenchymal stem cells through ERK1/2 kinase pathway. The results showed that BMP9 can activate ERK1/2 kinase through increase the phosphorylated form of ERK1/2 kinase. ERK1/2 kinase inhibitor PD98059 can increase the ALP activity, OPN expression and calcium deposition of C3H10T1/2 cells induced by BMP9. Furthermore, PD98059 also led to enhancement of Runx2 activity and activation of canonical Smad pathway stimulated by BMP9. When ERK1/2 kinase was silenced by RNA interference, BMP9-activated Smad pathway was further enhanced. Moreover, ALP activity, calcium deposition and in invo ectopic bone formation were accordingly increased along with knockdown of ERK1/2 kinase. Taken together, those results intensively suggested that BMP9 can activate ERK1/2 kinase, and inhibition of ERK1/2 kinase can enhance BMP9-induced osteogenic differentiation of mesenchymal stem cells. ERK1/2 are highly capable of negatively regulate BMP9-induced osteoblastic differentiation of mesenchymal stem cells.

Reviews and Monographs

Advances in microRNAs and TP53 Gene Regulatory NetworkGONG Zhao-Jian,HUANG Hong-Bin,XU Ke,LIANG Fang,LI Xiao-Ling,XIONG Wei,ZENG Zhao-Yang and LI Gui-Yuan  [Abstract][PDF][HTML]

New Research Advances in Genetics Associated With High-density Lipoprotein CholesterolLIU Xiao-Yan,LU Qian,CHEN Wu-Jun and TANG Chao-Ke  [Abstract][PDF][HTML]

Prediction of Long Non-Coding RNAs Based on RNA-SeqSUN Lei,ZHANG Lin and LIU Hui  [Abstract][PDF][HTML]

The Cognitive Mechanism and Neural Bases of Statistical LearningWU Qiu-Yan and DENG-Yuan  [Abstract][PDF][HTML]

Research Papers

The Temporal Responses of Neurons in The Primary Visual Cortex to Transient StimuliLI Xiao-Jian,JIANG Zhen and WANG Yi  [Abstract][PDF][HTML]

Inhibition of ERK1/2 Kinase Enhances BMP9-induced Osteogenic Differentiation of Mesenchymal Stem CellsSONG Tao,HE Juan-Wen,WANG Jin,TANG Min and LUO Jin-Yong  [Abstract][PDF][HTML]

Effects of GABA Signal on Mouse Placenta Establishment in Early-Middle PhaseLUO Wen-Ping,TAN Dong-Mei,YANG Gen-Ling,LU Jun-Jie,ZHAO Hai and TAN Yi  [Abstract][PDF][HTML]

Crystallization and Preliminary Crystallographic Studies of Active TNF-α-Inducing Protein From Helicobacter PyloriGAO Ming-Ming,ZHANG Ying and WANG Da-Cheng  [Abstract][PDF][HTML]

Intein-Fused Lucine Zippers Increase Plasma Coagulation Activity by Improving Protein Trans-Splicing in Dual-Vector Factor Ⅷ Gene Delivered MiceZHU Fu-Xiang,LIU Ze-Long,MIAO Jing,QU Hui-Ge and CHI Xiao-Yan  [Abstract][PDF][HTML]

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