Vol.44,No.7,2017

Other Issues:  
  
 
Cover Story:Intervertebral disc degeneration is believed to originate in the nucleus pulposus (NP) region, it is important to obtain greater numbers of active NP cells for the study and therapy of disc degenerative disease (DDD). Human induced pluripotent stem cells (iPSCs) are a powerful tool for modeling human development and disease, as well as for their potential applications in regenerative medicine. We isolated NP cells from DDD patients with our improved method, and reprogramed primary NP cells into iPSCs with Sendai virus vectors encoding 4 factors. Successful reprogramming of iPSCs was verified by specific surface markers and teratoma, and differentiation of iPSCs into NP-like cells was performed in culture plate and hydrogel, with skin fibroblast derived-iPSC used as control. It was demonstrated that iPSCs derived from NP cells were featured with normal karyotype, and showed expression of pluripotency markers and were able to form teratoma in nude mice. NP induction of iPSCs resulted in their expression of NP cell specific matrix proteins and related genes. NP derived-iPSCs without induction also showed a basal level of expression of some NP-like phenotype characteristics. What’s more, NP derived-iPSCs prefer to differentiate into NP-like cells in hydrogel rather than in culture plate.This is the first protocol for generation of iPSCs from NP cells of DDD patients, and we successfully differentiated this iPSCs into NP-like cells in hydrogel. This method opens a window in the treatment of DDD by using patient-specific NP cells in a relatively simple and straight forward manner.

Reviews and Monographs

The Complexity of RNA Translation: Non-translation, Part-translation, De Novo-translation and Over-translationHAO Ya-Jing, LUO Jian-Jun, ZHANG Bao and CHEN Run-Sheng  [Abstract][PDF][HTML]

Advance of Immunoregulatory Mechanisms of Long Non-coding RNAXIA Li and ZHOU Yu-Feng  [Abstract][PDF][HTML]

The Biological Functions of tRNA-derived Fragments and tRNA Halves,and Their Roles in The PathogenesisZHU Lin-Wen, XIE Yi and GUO Jun-Ming  [Abstract][PDF][HTML]

The Advancement of The Prediction Methods for DNA-binding Preferences of C2H2 Zinc Finger ProteinsSHEN Pan, YANG Dong and HE Fu-Chu  [Abstract][PDF][HTML]

Research Papers

LncRNA-GAS5 Inhibits Decay of microRNA-21 Imperfect Complementary Target mRNAsZHENG Wei, DONG Jie, LI Shao-Hua, DING Hong-Mei, LI Hui, HUANG Ai-Xue, XIA Wei, BAI Chen-Jun, GUO Xiao-Hua, LI Da, GENG Jie, LI Jie and SHAO Ning-Sheng  [Abstract][PDF][HTML]

Generation and Functional Characterization of Induced Pluripotent Stem Cells From Human Intervertebral Disc Nucleus Pulposus CellsWU Zhang-Song, LUO Zhi-Qiang, ZHANG Ming-Yu, LIAN Cui-Hong, LIN Xin-Yan, LIANG Yu-Hong, GU Hong-Sheng and ZHU Yan-Xia  [Abstract][PDF][HTML]

Accumulation of Simulated Pathological Level of Formaldehyde Decreases Cell Viability and Adhesive Morphology in Neuronal CellsWANG Jing, ZHOU Jun, MO Wei-Chuan, HE Ying-Ge, WEI Yan, HE Rong-Qiao and YI Fa-Ping  [Abstract][PDF][HTML]

Motion-in-depth Direction Discrimination in Dynamic Random Dot Stereogram: The Role of Visual Perceptual TrainingZHANG Di, Vincent Nourrit, SANG Xin-Zhu and Jean-Louis de Bougrenet de la Tocnaye  [Abstract][PDF][HTML]

Letter to Editor

Na+ Binding and pH-Dependent Transport in Nonlabens dokdonensis Rhodopsin 2ZHAO Hong-Shen and CHEN De-Liang  [Abstract][PDF][HTML]

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