Vol.46,No.4,2019

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Cover Story:To explore the effect and mechanisms of cytoplasmic M-CSF on glucose metabolism in human breast cancer MCF-7 cells, MCF-7 cells stable expressing cytoplasmic M-CSF were constructed. Relative ATP content was measured by ATP assay kit, glucose was measured using glucose assay kit and lactate was measured using lactate acid assay kit. The expression of HK2, PKM2 and GLUT-1 in three kinds of cell with LY294002 or API-2 was detected by West-blotting. The sensitivity of MCF-7 and MCF-7-M cells to 5-FU with the treatment of ATP depletion by 3-BrPA was observed by MTT assay. It was found that the ATP level of MCF-7-M cells was significantly higher than that of MCF-7 cells (P<0.05); 2-DG decreases the ATP level of MCF-7 and MCF-7-M cells, and the effect of lowering the ATP level of MCF-7-M cells is more obvious (P<0.01). The glucose uptake and lactate secretion of MCF-7-M cells were significantly higher than those of MCF-7 cells (P<0.01). After the treatment with API-2, the glucose consumption and lactate secretion of MCF-7 and MCF-7-M cells were significantly reduced (P<0.01). The expressions of GLUT-1, HK2 and PKM2 in MCF-7-M cells were significantly higher than those in MCF-7 cells (P<0.01). Both LY294002 and API-2 inhibited the expression of GLUT-1 in MCF-7-M cells (P<0.05). After the treatment with 3-BrPA, the drug sensitivity of MCF-7-M and MCF-7 cells to 5- FU was significantly enhanced (P<0.01). In conclusion, cytoplasmic M-CSF activates glycolysis by induce GLUT-1, HK2 and PKM2 protein expression in MCF-7 cells; PI3K/AKT signaling involves the pathway that glycolysis was activated by cytoplasmic M-CSF in MCF-7 cells.

Reviews and Monographs

Research Progress of CAR-T Specific Therapy to TumorMEI En-Dian,MA Jia-Bing,GAO Jia-Dong,LIU Yi-Xuan,QIAN Cheng,LIU Li and WEI Xu-Bin  [Abstract][PDF][HTML]

Single Cell Whole Genome Amplification Technology and ApplicationXU Xiao-Li,WU Ling-Juan and YAN Ren-Xiang  [Abstract][PDF][HTML]

Progress in Biomedical Applications of Magnetic NanomaterialsTANG Qian-Qian,ZHANG Yi-Fan,HE Yuan,PENG Ming-Li,ZHAI Gao-Hong and FAN Hai-Ming  [Abstract][PDF][HTML]

Magnetic Hyperthermia Using Iron Oxides NanoparticlesXU Huang-Tao,REN Wei and PAN Yong-Xin  [Abstract][PDF][HTML]

Progress About The Role of Protein Acetylation on AutophagyCHEN Yu,WAN Qun,JIN Lu-Qi,YAN Chun-Lan and DING Shi-Ping  [Abstract][PDF][HTML]

The Neurophysiological Mechanism of Emotional Mimicry: From The Mirror Neuron System to Neuroendocrine NetworkXU Xiao-Hui and HU Ping  [Abstract][PDF][HTML]

Research Papers

The Effect and Mechanism of Cytoplasmic M-CSF on Glucose Metabolism in Human Breast Cancer MCF-7 Cells via PI3K/AKTTIAN Yun,LIU Zhen,NING Qian,MO Zhong-Cheng,ZHANG Meng-Xia and TANG Sheng-Song  [Abstract][PDF][HTML]

Mining and Functional Analysis of Chromatin Interaction-related Transcription FactorsWANG Qi,DING Yan-Shu,GENG Bao-Bao and NIE Yu-Min  [Abstract][PDF][HTML]

Techniques and Methods

Application in Localization of E. coli sRNA SgrS CombiningSuper-resolution Microscopy With smFISH MethodWANG Jing,RUAN Chong-Mei,BAI Yuan-yuan,HAN Yan-Ping and YANG Rui-Fu  [Abstract][PDF][HTML]

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