Vol.46,No.5,2019

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Cover Story:Adenylate cyclase Ⅲ (AC3) is an important component of odorant perception signaling in the olfactory system. The thinness of main olfactory epithelium (MOE) become thin with ageing, and the gene expression profile alters after AC3 deletion. DNA methylation plays a key role in animal development and regulation of gene expression. In the present study, whether the DNA methylation level of gene promoter, as well as their associations with the expression of the genes in MOE will be altered after AC3 deletion, was investigated by using methylated DNA immunoprecipitation chip (MeDIP-chip), methylation-specific PCR (MSP) and real-time fluorescence quantification PCR. The data showed that the DNA methylation levels of promoters of 1 978 genes were altered in AC3-deficient mice, accounting for 9% of the total number of genes. Of which 727 genes with their promoter’s DNA methylation levels were elevated, 1 251 genes with their promoter’s methylation levels were lowered. The functions of these genes are mainly involved with olfactory receptor, neurodevelopmental, cAMP signaling pathway, ATP-binding, calcium regulation, acetylation modification, and transcription factors. It was further confirmed by MSP that methylation levels of promoter of the olfactory receptor genes Olfr1153, Olfr231, Olfr378, Olfr651 and Olfr691 were increased, whereas methylation level of the promoters of Cngb1, Pde4a and Olfr1394 were decreased. In line with MSP results, qRT-PCR data showed that the expression levels of Cngb1, Hcn4, Olfm1, Olfr1394, Olfr1153, Olfr231, Olfr378 and Olfr691 were significantly decreased, whereas the expression levels of Pde4a and Olfr651 were significantly increased. In conclusion, the methylation levels of promoters of olfactory receptor genes, neurodevelopmental related genes and cAMP signaling pathways in MOE are modified significantly after AC3 deletion, which affected the transduction of signal pathways such as nucleotide excision and repair, DNA replication and mismatch repair, thus comprehensively regulating the number and level of gene expression in MOE of mice.

Reviews and Monographs

Progress of Mechanism of Tumor Immune Escape and Tolerance Mediated by ExosomeYIN Chun-Lai,ZHAO Xue-Mei,HOU Zhao-Hua,ZHANG Jian and HAN Qiu-Ju  [Abstract][PDF][HTML]

Advances in The Prediction of Antigenic Peptides in Personalized Tumor Neoantigen VaccineWANG Guang-Zhi,LI Yu-Yu and XIE Lu  [Abstract][PDF][HTML]

Gender Difference in The Pathogenesis of Alzheimer’s DiseaseWANG Zhen,GAO Pei-Pei,PENG Yun-Hua,LIU Jian-Kang and LONG Jian-Gang  [Abstract][PDF][HTML]

The Mechanisms of Oxytocin in Alleviating Depressive SymptomsHU Qian-Yu,HU Ling-Jie,JIE Jun-Jin,SHEN Si-Hong and CHEN Xiao-Wei  [Abstract][PDF][HTML]

Relationship Between Neutrophil Extracellular Traps and Pulmonary Inflammatory DiseasesCHEN Xi,XIAO Jian,LI Yuan-Yuan,CHEN Qiong and HU Cheng-Ping  [Abstract][PDF][HTML]

Research Papers

Effect on DNA Methylation in The Main Olfactory Epithelium of Mice With Deletion of Adenylate Cyclase ⅢZHOU Yan-Fen,WANG Ya-Wen,WANG Xiao-Ting,SHU Li,LI Shu-Juan and WANG Zhen-Shan  [Abstract][PDF][HTML]

Regulation of Glycosphingolipid GM1 on The Density Dependent Inhibition of Human Breast Cell Growth In VitroZHUO Ding-Hao and GUAN Feng  [Abstract][PDF][HTML]

High-accuracy Splice Site Prediction Based on Statistical Difference Table and Weighted VotingZENG Ying,CHEN Yuan and YUAN Zhe-Ming  [Abstract][PDF][HTML]

Anti-inflammation Effect and The Mechanism of Suitable Concentration of Short-chain Fatty Acids Mixture on MicrogliaJIA Xue-Bing,ZHOU Zhi-Lan,ZHANG Bo-Ping,ZHAO Li-Ping,QIAO Chen-Meng,SUN Meng-Fei,SUN Xiu-Lan,CHEN Yong-Quan,CUI Chun and SHEN Yan-Qin  [Abstract][PDF][HTML]

Techniques and Methods

Preparation of Thymosin-α1 Using a One-step Heat-treatment Method and CNBr CleavageZHANG Chong,TIAN Liu-Yang and WENG Hai-Bo  [Abstract][PDF][HTML]

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