Antisense can be used to control the expression of specific genes. When targeted to specific messenger RNAs or specific sequences of the DNA double helix, antisense inhibit translation or transcription. Both strategies can beapplied to control the expression of oncogenes and growth factors in tumor cells. Here, the application of antisense was reviewed in cancer research briefly. a. Inhibition of oncogene and growth factor expression to suggest their function in tumor cells. b. Discrimination between proto-oncogene and activated oncogene. c. Problems and approaches in antisense gene therapy.