Using HRP-conjugated goat anti-human apoAI-IgG, an enzyme-linked immunosorbent receptor assay was developed for measurement of high density lipoprotein (HDL) receptors on rabbit liver plasma membranes. A curve of anti apoAI-IgG binding to known amounts of HDL was constructed to quantify the HDL bound. Parallel samples with 25-fold excess goat plasma HDL were assayed to detect nonspecific binding. The results showed that the K_d and B_max of rabbit liver plasma membrane HDL receptors were (7.17±1.18)mg/L and (622.5±146.1)mg/L respectively (n=7).