A cDNA (VvSUC27) of grape sucrose transporter was inserted into an expression vector pBI121 in the anti-sense orientation under the control of the cauliflower mosaic virus 35 S promoter, and subsequently transferred into tobacco(Nicotiana tobacum cv. Samsun). The plantlets of the transformed anti-sense VvSUC27 cDNA tobacco were grown normally on MS media containing 20g/L sucrose, but it was found that roots of the transformants developed slowly and the leaves have more chloroplasts when observed under slice. Sucrose content in the roots of the transformants was only 51% of the controls. The capability of sucrose uptaking and transporting into leaves among the transformed tobaccos was greatly decreased.