Brain stimulation is an important means in neuroscience research. Traditional brain stimulation such as transcranial magnetic stimulation and transcranial electrical stimulation can regulate motor function (including alleviation of motor disorders and improvement of motor ability), but they have low spatial resolution and are unable to stimulate deep brain tissue. In recent years, some brain stimulation methods with accurate positioning such as deep brain stimulation (DBS), optogenetics, transcranial ultrasound stimulation (TUS), temporal interference (TI) stimulation have achieved rapid development, showing higher spatial resolution and reaching deeper brain tissue. We summarized the principles and characteristics of the above brain stimulation methods, and progress in the regulation of these brain stimulation on motor function as well as their challenges and prospects, for providing more neuroscience research tool and clinical intervention means.