固体支撑的自组装的双层类脂膜
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SELF-ASSEMBLING BILAYER LIPID MEMBRANES ON SOLID SUPPORT
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    摘要:

    具有通常BLMs某些相似特性的固体支撑的双层类脂膜(S-BLM)能够通过两步自组装到新劈开的金属丝上面。如:(1)包有聚四氟乙烯的铂丝头部浸在类脂溶液里,用解剖刀把顶部切开;(2)包有类脂溶液的新铂丝末端转移到0.1mol/L KCl溶液里,电测定证实,数分钟后,在金属丝末端自动地形成了稳定的类脂双层。本文报道了这种固体支撑的BLM(S-BLM)在检测Pb2+离子中的应用。S-BLM为液晶结构,它可用于基础研究、生物传感器和分子电子器件等技术上的应用。

    Abstract:

    Solid supported bilayer lipid membranes (S-BLM) that posses some properties similar to those of conventional BLMs can be self-assembled on a freshly cleaved metal wire by a twostep procedure: 1. The tip of a Teflon-coated platinum wire, while immersed in a lipid solution, is cut off with a scalpel; 2. The new tip of the wire, having become coated with lipid solution, is transferred into 0.1 mol/l KCl. After a few minutes, a stable lipid bilayer forms spontaneously on the tip of wire, as verified by electrical measurements. This paper reports an application of such a supported BLM (S-BLM) in the detection of Pb2+ ions. The S-BLM is liquidcrystalline in structure, which makes it amenable to modification for basic studies, as well as for technological applications such as biobensors and molecular electronic devices.

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田心棣,Z. SALAMON.固体支撑的自组装的双层类脂膜[J].生物化学与生物物理进展,1990,17(5):359-363

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  • 收稿日期:1990-02-08
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