以家蚕核多角体病毒(BmNPV)为载体,在家蚕幼虫或家蚕培养细胞系中表达的外源基因越来越多,其表达的产物已涉及到医用药物、医疗诊断、疫苗生产、生物防治等诸多领域,文章就BmNPV的特性及其基因组构造,多角体蛋白基因的特性,重组BmNPV的构建及其在家蚕幼虫体内和细胞系中的表达,BmNPV-家蚕表达系统的外源蛋白生产效率及其应用等各个方面作了全面、系统的综述.
More and more foreign genes have been expressed in the silkworm larvae or silkworm cell lines using the Bombyx mori nuclear polyhedrosis virus (BmNPV) as a expression vector.The expressed products involve in many fields such as pharmaceutics, medical diagnosis, vaccine production and biological control. The characteristics of BmNPV and its genome structure,characteristics of polyhedrin gene,construction of recombinant BmNPV and its expression in the silkworm larvae and cell line, and efficiency of production for the foreign gene products expressed in the silkworm-BmNPV system and application of the expressed product were described systematically in the review.
张雨青.以核多角体病毒为载体在家蚕中生产外源蛋白[J].生物化学与生物物理进展,1994,21(5):406-410
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