用混合去垢剂增溶低离子强度条件下的温和电泳方法,对菠菜叶绿体类囊体膜和不同PSⅡ制剂进行了色素蛋白复合体组成的分析.并结合SDS聚丙烯酰胺凝胶电泳和吸收光谱对部分色素蛋白复合体进行了鉴定.此法简便、迅速、游离色素少.
The chlorophyll-protein complexes of thylakiods and those of various PSⅡ preparations were analyzed by a low ionic strength native“green gel”system after solubilization by a mixture of detergents,with very little release of free pigment.The subunit composition and absorption spectra of some chlorophyll-protein complexes were studied also.This new native green gel system is simple and rapid,with release of very little free pigment.
杜林方,林宏辉,潘用华,梁厚果.一种用于色素蛋白分离的新凝胶系统[J].生物化学与生物物理进展,1995,22(3):268-272
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