Attributing to the progress in the Genome Project, more and more gene sequences, most of which are not known any information about function, have been accumulated in database. So analysing the function of those genes will become the main goal after the Genome Project is finished. Some strategies and procedures for large-scale functional analyses of genes, such as gene disruptions, serial analysis of gene expression,high density cDNA hybridization and proteome analyses, are introduced. Furthermore, the prospects of large-scale gene functional analyses are also discussed.