用随机扩增多态性DNA产物做探针产生鸡的DNA指纹图
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国家自然科学基金资助项目(39570386).


DNA Fingerprinting in Chicken with Probes Derived from Randomly Amplified Polymorphic DNAs-PCR Products
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    摘要:

    我们用12个随机扩增多态性DNA(RAPD)引物对来自不同品系的4只鸡进行了RAPD分析,在扩增出的共99条带中,表现多态性的带为38条,占总带数的38%.回收了4个表现个体特异性的RAPD产物,当用鸡的基因组总DNA探针与它们杂交时,其中3个表现阳性,说明RAPD方法扩增出的高变异产物含有重复序列.用含重复序列的个体特异性RAPD产物作探针,与无关个体鸡基因组DNA的HaeⅢ酶切产物进行DNA印迹,获得了变异性较高的DNA指纹图谱.因此,高变异的RAPD产物可以有效地用作DNA指纹探针.

    Abstract:

    Four chickens, each from a different strain, were analyzed using randomly amplified polymorphic DNAs(RAPD) technique with 12 primers. 38 of 99(38%) detected fragments showed polymorphic. Four individual-specific fragments were recovered and probed with labeled whole genome of chicken. Positive signal was detected in three of the fragments, suggesting that hypervariable fragments generated by RAPD contain repetitive sequences. Highly variable DNA fingerprints were generated when such repetitive sequence containing fragments were used as probe to hybridize to HaeⅢ-digests of random chickens’DNA. Therefore, RAPD products that are highly variable among individuals can be used as DNA fingerprinting probes.

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杨绍清,孟安明.用随机扩增多态性DNA产物做探针产生鸡的DNA指纹图[J].生物化学与生物物理进展,2000,27(4):426-429

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  • 收稿日期:1999-07-19
  • 最后修改日期:1999-11-24
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