Using bioinformatics analysis and 5′RACE technology, a new gene Mip1 which is up-regulated during rat myocardial ishemia-reperfusion had been cloned. The full length of Mip1 was proved by RT-PCR sequencing and multiple tissue Northern blot. Bioinformatics analysis indicate that Mip1 is located in chromosome 1q12, including 5 exons and 4 introns. The full-length cDNA has an open reading frame of 1 827 bp, which encodes 608 amino acids. There is a KRAB domain at the N terminal of the hypothetical protein and 14 successive C2H2 type zinc finger domain at the C terminal of the protein. There is a bipartite nuclear targeting sequence from amino acid 277 to 193. Mip1 is expressed abundantly in brain and heart and seldom in other tissues. It is worthwhile to further study the biological functions of Mip1.