Erythropoietin (EPO) plays an important role in modulating proliferation and differentiation of erythrocytes. The fetal liver stromal cell lines(FLSCs) expressing EPO has been established steadily by lentiviral system. The EPO gene was cloned from human fetal liver by RT-PCR. The EPO recombinant lentiviral plasmid was steadily transfected into FLSCs. The efficiency of virus transfection was identified by expression of enhanced green fluorescence protein(eGFP) analyzed by fluorescence microscope, then the high eGFP espression FLSCs were sorted by fluorescence-activated cell sorting (FACS) according to strong eGFP expression. Analysis of strong eGFP expression was detected by RT-PCR and ELISA. The EPO expression at mRNA level of strong eGFP expression FLSCs are 5.63 and 5.71-fold for the FLSCs no transfected and the FLSCs transfected by the control lentivirus. And at protein level, the content of EPO expression is 263 U/L. Then the supernatant from the EPO transfected FLSCs could induce the CD34+ cell differentiated into hematopoietic cell, especially erythrocytes. This would provide an alternative for cell therapy and blood cell transfusion.