新一代高通量RNA测序数据的处理与分析
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国家自然科学基金资助项目(60702002, 60721003, 30873464, 60905013)和东南大学生物电子学国家重点实验室开放研究基金资助项目


A Review on The Processing and Analysis of Next-generation RNA-seq Data
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This work was supported by grants from The National Natural Science Foundation of China (60702002, 60721003, 30873464, 60905013) and The Open Research Fund of State Key Laboratory of Bioelectronics, Southeast University

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    摘要:

    随着新一代高通量DNA测序技术的快速发展,RNA测序(RNA-seq)已成为基因表达和转录组分析新的重要手段.RNA-seq技术产生的海量数据为生物信息学带来了新的机遇和挑战.有效地对测序数据进行针对性的生物信息学处理和分析,成为RNA-seq技术能否在科学探索中发挥重大作用的关键.以新一代Illumina/Solexa测序平台所产生的数据为例,在扼要介绍高通量RNA-seq测序流程的基础上,对RNA-seq数据处理和分析的方法和现有软件做一个较为全面的综述,并对其中有待进一步研究的问题进行展望.

    Abstract:

    With the rapid development of the next-generation sequencing (NGS) technology, high-throughput RNA sequencing or RNA-seq is becoming a key experimental approach in the study of gene expression and transcriptome. The overwhelming amount of RNA-seq data brings new opportunities and challenges for bioinformatics. The efficient and effective processing and analysis of RNA-seq data is becoming the bottleneck for turning the possibilities provided by the new technology into real scientific discovery. A general description of the typical RNA-seq protocol was given. A complete review of major methods and available software in the processing and analysis of RNA-seq data were presented, using the Illumina/Solexa platform as an example. Questions that are still open and awaiting further research are also discussed.

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王曦,汪小我,王立坤,冯智星,张学工.新一代高通量RNA测序数据的处理与分析[J].生物化学与生物物理进展,2010,37(8):834-846

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  • 收稿日期:2010-03-25
  • 最后修改日期:2010-04-15
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  • 在线发布日期: 2010-05-12
  • 出版日期: 2010-08-20