Whether the immunogenicity of DNA vaccine can be further increased by novel deliver system- bacterial ghost is discussed. First, the bacterial ghost displaying ureB epitope by CS3 fimbriae was constructed, namely, plasmids pCSXureB harboring ureB epitope and plasmids pAcYclysis harboring lysis cassette were transformed into E. coli together. The recombinant bacterial ghosts were then enfolded the Helicobacter DNA vaccine pcDNAKAT by incubating at 24℃ for 30 min. It was confirmed by DNA agarose electrophoresis,fluorescent microscope that plasmids pcDNAKAT could bound to bacterial ghost unspecificly, successful loading also resulted in the fluorescence shift indicated by the MFI of flow cytometric analysis. The anti-katA antibody titer of mice immunized with packaged DNA vaccine was 1∶(520 ± 54), which was significantly higher than that of control groups(P = 0.0058), which was indicated strongly that bacterial ghosts could deliver DNA vaccine effectively.