In this paper, stable epileptiform discharges were induced on rat hippocampal slices by using the electrode array detection technique. To analyze and study the parameters of the hippocampal slices, the starting site of epileptiform discharges, the propagation direction and transmission rate, and the power spectral density of each frequency band during the inter-ictal discharges (IID) and ictal discharges (ID) when 130 Hz high-frequency stimulation (HFS) was applied to CA3 region. The results showed that HFS could effectively reduce the amplitude and duration of seizures, increase latency time, and inhibit the transition of epileptiform discharges from IID to ID. In conclusion, HFS resists epilepsy through promoting the inhibitory transmission system and resisting the excitatory connections between hippocampal neurons.