Objective Based on bioelectrical impedance spectroscopy (BIS), we propose a label-free method for real-time detection of biological cell activity. The method determines whether the cells are active or not based on the differences in electrical properties of the cells at different concentrations, physiological and pathological states. It is used to assist physicians to quickly and precisely locate the scalded tissues in patients and achieve effective excision during clinical surgery.Methods Active zebrafish embryonic stem cells were used to simulate human scalded tissues, and bioimpedance spectroscopy was used to identify the physiological state of the cellular tissues.Results There were significant differences in the impedance amplitude changes of the cells in different states, from which it could be found that the impedance amplitude of the active cells at the same concentration was on average 17.25% higher than that of the dead cells, and the relaxation frequency of the active cells occurred 25% earlier than that of the dead cells.Conclusion The experimental data showed that the bioimpedance spectroscopy method can effectively distinguish two types of physiological states of embryonic cells; according to the clustering region, it can be seen that the BIS assay has obvious ability to distinguish cell activity and concentration, which can quickly assist physicians to complete the detection of scalded tissues of patients theoretically.