2022年第49卷第10期目录
|
|
封面故事:蛋白质糖基化与肿瘤的密切相关,N-连接糖基化可能在未来做为临床诊断标志物,因
此对肿瘤患者血清或组织糖链标志物的开发具有重要意义。程颖等以临床组织FFPE切片为对象,
对组织中糖蛋白质N-连接糖链进行原位释放,并结合高分辨率质谱MALDI-TOF/TOF-MS分析方
法,检测肿瘤组织和癌旁组织FFPE的N-连接糖链修饰水平。经过多样品的系统分析发现,肿瘤组
织中大部分高甘露糖型修饰水平显著上升,部分复杂型N糖链修饰水平显著下降,其中双天线型N-
聚糖N3H4和N4H4能够有效区分膀胱癌与癌旁组织。该方法能够直接从组织切片上酶解释放N-聚
糖,较传统N-聚糖检测方法具有简便快捷,且能够很好地与临床组织病理分析技术相结合,在膀
胱癌的辅助诊断中具有潜在的应用价值。该文所鉴定的膀胱癌组织N-聚糖糖组学数据或可对后续
膀胱癌蛋白质糖基化相关研究提供支撑。
(程颖,孙承文,秦艳,时帅,李岳阳,樊启高,杨刚龙,高晓冬. 基于FFPE 组织切片的膀胱癌N-连接糖
链原位酶解及分析,本期第2001~2014 页)
Cover Story:Objective To investigated the N-linked glycans in bladder cancer FFPE tissue sections, and explored abnormal N-linked glycans modification in bladder cancer FFPE tumor tissues sections.Methods An experimental procedure for in situ extraction of N-linked glycans based on FFPE tissue sections was developed. FFPE tissue sections were digested by PNGase F and liberated N-linked glycans. Permethylation was performed to modify the free end of the N-linked glycans. The relative intensity of N-linked glycans was detected by MALDI-TOF/TOF-MS. Database matching was performed to determine possible glycoforms of N-linked glycans. The accuracy of significantly different N-linked glycans as a predictive bladder cancer biomarker were predicted by ROC analysis.Results The data of MALDI-TOF/TOF-MS detection of premethylation-modified N-linked glycans showed that, in tumor and peritumoral FFPE tissue sections of 16 patients with bladder cancer, the relative intensity of N-linked glycans N2H6, N2H7, N2H8, N2H9 (high mannose) and N5H6F1 (complex) were increased significantly in the tumor tissues of bladder cancer. At the same time, N2H5 (high mannose), N3H5(hybrid) and type N3H4, N4H4, N5H6F1S2 (complex) N-linked glycans were significantly decreased. ROC analysis showed that the biantennary N-linked glycan N3H4 (AUC=0.90) and N4H4 (AUC= 0.91) were reliable in distinguishing tumor and peritumoral tissue of bladder cancer patients separately or combined together, and these N-linked glycans may become a potential biomarker for bladder cancer.Conclusion Abnormal N-glycosylation of proteins in bladder cancer FFPE tumor tissue, N-linked glycans N3H4 and N4H4 may be potential biomarkers of bladder cancer.
|
新型冠状病毒肺炎研究专题
综述与专论
研究报告
技术与方法
科教融合
|
|