2023年第50卷第3期目录
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封面故事:深部脑刺激利用持续的高频电脉冲刺激调控神经元活动,用于治疗多种脑神经系统疾
病。为了深入了解高频电刺激的作用机制,该文利用电流源密度分析法,研究神经元自身的轴突
受到高频刺激期间,其胞体响应的改变。在大鼠海马CA1区的锥体神经元轴突上施加1 min脉冲频
率为100 Hz的高频电刺激,利用线性垂直排列的多通道微电极阵列,在胞外记录这些神经元胞体
附近各结构分层上的诱发电位,并计算其电流源密度,分析刺激期间胞体附近动作电位的生成和
传导。实验结果表明,轴突上的高频电刺激会减小锥体神经元胞体对于其轴突上的逆向兴奋和树
突上的顺向兴奋的传导速度。并且,这种胞体变化的发生和恢复都要慢于刺激诱导的轴突变化
(即轴突阻滞)。此研究结果对于揭示神经电刺激的作用机制,促进新型刺激模式的开发与应用具
有重要意义。
(徐义鹏,封洲燕,袁月,胡一凡,叶翔宇,王兆祥. 海马区锥体神经元轴突高频电刺激对胞体的影响,
本期第561~572页)
Cover Story:Objective Deep brain stimulation (DBS) utilizes sustained high-frequency stimulation (HFS) of electrical pulses to modulate neuronal activity. The therapy is expected to be used to treat more brain disorders. To deeply understand the mechanisms of the HFS to advance the DBS development, the present study investigates the effect of axonal HFS on neuronal somata during HFS-induced axonal block.Methods Antidromic high-frequency stimulation (A-HFS) with a 100-Hz pulse frequency and a 1-min duration was applied at the axons of pyramidal neurons in the hippocampal CA1 region of anesthetized rats. To investigate the responses of somata, a multi-channel microelectrode array with a vertical linear configuration was implanted to record the evoked potentials in the lamellas around the somata of CA1 pyramidal neurons at the upstream area of stimulation site, including the antidromic population spikes (APS) evoked by the pulses of A-HFS as well as the orthodromic population spikes (OPS) evoked by orthodromic test pulses applied during the A-HFS. Current-source densities (CSD) of the evoked potentials were calculated to evaluate the generation and propagation of action potentials around the somata of pyramidal neurons during A-HFS.Results A-HFS on the axons of pyramidal neurons slowed down the propagation speed of both antidromic and orthodromic excitations around somata. In addition, the occurrence and recovery of the changes of somata were slower than the A-HFS-induced axonal block.Conclusion Axonal HFS can induce soma alterations that might be caused by changes in membrane potentials nearby somata. The finding is helpful for deeply revealing the mechanisms of electrical stimulations of brain nervous system.
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综述与专论
研究报告
技术与方法
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