建立了Ⅱ型胶原的提取和纯化方法,观察大鼠软骨Ⅱ型胶原体外胶原原纤维形成的动力学、胶原原纤维的稳定性和超微结构。结果表明,Ⅱ型胶原静止期、增长期分别为15、120min,增长期凝胶化速率0.0060,凝胶可复性13.58%,胶原原纤维呈典型的具有64nm周期横纹的自然纤维结构,但纤维直径较体内增长。这些对研究体内胶原原纤维的形成过程及调节机制具有重要意义。
The extraction and purification methods of type Ⅱ collagen from the rat cartilage were established. Kirietics of fibrillogenesis, stability and ultrastructure of fibils of type Ⅱ collagen of cartilage from rats were investigated in vitro in this paper. The results indicated that the tag phase and growth phase were 15 and 120 min respectively, gelatinization rate in growth phase was 0.0060, gelatin reversibility was 13.58%, and collagen fibrils showed typically banding pattern staggered by 64nm, especially for the native fibrils, but the diameters of these fibrils were larger than those in vivo. These data are of importance for studying the formation and regulation mechanism of collagen fibrils in vivo.
付志厚,张矢远.大鼠软骨Ⅱ型胶原体外胶原原纤维的形成[J].生物化学与生物物理进展,1992,19(2):114-118
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