To elucitate characteristics of the novel GGTP specific to liver cancer cells,GGTPsfrom human fetal liver and hepatoma tissues were purified by Triton X-100 and deoxy-cholate extraction,cold acetone precipitation,glutathione treatment and bromelain digestionfollowed by Sephadex G-150 gel filtration and DEAE-cellulose DE-52 chromatography. The enzymes purified from two sources displayed a pI of 4.2 and an apparent M_r ofabout 92kD,as judged by polyacrylamide gel electrophoresis,which consists of two non-identical glucopeptides with M_r of 64 kD and 27 kD, respectively. The purified GGTPs were used to produce antibodies in rabbits. The antisera with titers of 1:16 were observed to be capable of being absorbed by the constituents of the novel GGTP, Ⅱ and Ⅱ', in sera of patients with hepatoma. Furthermore, they could form in the double diffusion test a smoothly fused precipitating line with two kinds of purified antigens. These results show that GGTPs from human hepatoma and fetal liver tissues are of immunological identity.