Comparison of the Effects of two Synthesized Inhibitors of Respiratory Chain on Heart Muscle Preparation
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摘要:
用抑制剂作为分子工具研究呼吸链的电子传递机制已有相当长的历史,电子传递链各个区段均有酶专一的抑制剂被发现和使用.鉴于呼吸链中三个与泛醌反应相关的酶[还原辅酶Ⅰ:泛醌还原酶(NADH-Q reductase NQR)、琥珀酸:泛醌还原酶(succinate-Q reductase SQR)和泛醌:细胞色素 c 还原酶(QH2-cytochrome c re-ductase QCR)]均催化同一底物反应,从酶学角度看应存在一类抑制剂能对三个催化泛醌反应的酶兼有抑制作用.经合成和筛选发现3-硝基-N-十二烷基水杨酰胺和2-羟基-3-N-十二烷基酰胺吡啶具备这类性质,它们对催化泛醌反应的三个酶都有抑制作用,而对与泛醌无关的末端氧化酶(cytochrome c oxidase)无任何作用.3-硝 基-N-十二烷基水杨酰胺对检测的心肌制剂各段酶活性的抑制能力均强于2-羟基-3-N-十二烷基酰胺吡啶.
Abstract:
The inhibitory effects of two synthesized 3-nitrosalicyl-N-dodecylamide and 2-hydroxy-3-N-dodecylamide-pyridine on the respiratory chain had been compared by using heart muscle preparation from pig heart.3-nitrosalicyl-N-dodecylamide showed stronger effect than 2-hydroxy-3-N-dodecylamide-pyridine on all the three Q-related enzymes of NADH-Q reductase,suceinate-Q reductase and QH2-cytochrome c reductase.No any inhibitory effect of this two compounds on the terminal oxidase had been observed.The hydroxy groups in the rings of both compounds may be involved in the inhibitory effect through their disturbance on the hydration and dehydration of ubiquinone.
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