PCR-single strand conformation polymorphisms is a powerful method for screening mutations and widely used in studying mutations of oncogene and tumor suppressor gene.The ordinary PCR-SSCP needs the use of radioactivity and sequencing appratus, thus compromises its application.Here,a non-radioaltive asymmetric PCR-SSCP was established.Single-stranded DNA was generated by asymmetric PCR, seperated by mini PAGE and silver stained. The exon 5,6,7,8 of p53 gene in four cell lines of nasopharyngeal carcinoma-CNE1, CNE2, HK1 and SUNE1 were investigated. The method was proved to be succssful in screening mutations.