国家自然科学基金资助课题.
介绍了一种在热启动PCR中,以Dig-11-dUTP部分代替dTTP,从少量基因组DNA中快速制备大量的地高辛标记的探针的方法,此探针灵敏度达0.03pg,并只和相关的DNA特异杂交.
A method for generating a nonradioactive digoxigenin labeled probes is described.It was achieved by substituting the dTTP with Dig-ll-dUTP and utilizing human genome DNA as template during the Hot Start PCR.
李立家,张锡元,姜海波.热启动PCR快速制备地高辛标记探针[J].生物化学与生物物理进展,1994,21(6):557-558
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