用固相多肽合成法合成了生长激素释放肽(GHRP),这是一种含D-型氨基酸的外源性激素,具有促进脑下垂体分泌生长激素功能的人工合成六肽,其氨基酸序列为His-D-Trp-Ala-Trp-D-Phe-Lys-NH2.观察了使用不同剂量的GHRP对不同日龄小鼠的促生长效应,当使用最佳剂量(1000μg/kg)时,可使25日龄小鼠体重较对照组增加14.8%,同时发现鼠龄越小对GHRP越敏感,当使用剂量高达10mg/kg时仍然安全无毒.
Growth hormone releasing peptide (GHRP) was synthesized.It is an exogenous hormone promoting pituitary to release growth hormone.The amino acid sequence of GHRP is His-D-Trp-Ala-D-Phe-Lys-NH2.The synthesis was carried out with SPPS and Boc strategy.The amino acid composition was found in agreement with its caculated values. The purity was 97.7%.The bioassays showed that it enhanced the growth of young mice significantly and this effect depends on the dosage of injection. GHRP is more sensitive to the younger mice.
胡晓愚,王勤,王锐,高立伟,沈剑敏,王永谦.生长激素释放肽的合成及促生长活性[J].生物化学与生物物理进展,1995,22(6):549-552
复制生物化学与生物物理进展 ® 2024 版权所有 ICP:京ICP备05023138号-1 京公网安备 11010502031771号