采用改良的Grace层析方法,经一次DEAE-Sephadex A50柱层析即从人脑中纯化了神经元特异性烯醇化酶,比活力为92.1U/mg,纯化倍数为59.4.该酶纯化后,经SDS-聚丙烯酰胺凝胶电泳鉴定为单一蛋白质谱带.此外,还测定了其部分理化性质,其亚单位分子量为45000,等电点pI为4.7,氨基酸组成分析表明其为一种酸性蛋白质;对2-磷酸甘油酸的Km值为5.6×10-4mol/L.
A simple and convenient method for the purification of neuron specific enolase (NSE)from human brain is described through only one DEAE-Sephadex A50 chromatography.The specific activity is 92.1U/mg and increase of purity 59.4 fold.Certain biophysical and biochemical features were also studied.The molecular weight of the subunit of NSE is 45 000 and pI is 4.7.Amino acid composition analysis showed that NSE is an acidic protein.
郭健,张世明,周润琦,陈石根,韩一平.人脑神经元特异性烯醇化酶的纯化方法[J].生物化学与生物物理进展,1995,22(6):552-555
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