采用脉冲电场凝胶电泳法检测H2O2-Fe3+体系产生的OH·对人淋巴细胞DNA的双链断裂损伤.H2O2-Fe3+浓度与DNA双链断裂呈明显量效关系;随OH·作用时间延长,细胞DNA双链断裂加重;过氧化氢酶对OH·损伤有明显抑制作用.脉冲电场凝胶电泳法可检测到的H2O2和FeCl3引起细胞DNA双链断裂的最低浓度为0.3 mmol/L和6 μmol/L.
Pulsed field gel electrophoresis (PFGE) was used to quantitatively measure the double-strand breaks (dsbs) of DNA damage in human lymphocytes induced by hydroxyl radicals generated from H2O2-Fe3+ system.It was shown that there was a significant dose-dependent relationship between concentration of H2O2-Fe3+ and the content.of DNAdsbs occuring in cells. Moreover, the DNA damage in terms of dsbs became heavier as the time of incubating cells with H2O2-Fe3+ was extended but could be inhibited by catalase considerably.The sensitivity of PFGE was estimated by the concentration of H2O2-Fe3+ which were 0.3 mmol/L and 6 pmol/L, respectively.
隋建丽,吕星,周平坤,方允中. H2O2-Fe3+所致人淋巴细胞DNA双链断裂损伤[J].生物化学与生物物理进展,1996,23(1):59-62
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