国家“863”生物高技术(863-102-19-6)资助项目.
为了阻断家蚕核多角体病毒(BmNPV)的基因表达,以BmNPV的即刻早期蛋白基因(IE)为靶序列,设计了三联ribozyme.体外切割反应表明,该ribozyme能特异地切割靶序列的mRNA;细胞实验表明,细胞中表达的ribozyme也能够特异地切割靶序列,从而使受BmNPV感染的Bm-N细胞中的多角体减少约30%.
In order to evaluate the effect of ribozymes on suppressing BmNPV, triplet ribozyme (R426) targeting the BmNPV immediate early gene (IE) was designed. In vitro cleavage experiment showed that R426 transcript was able to cleave the target sequence specifically. Expression experiment indicated that R426 extracted from Bm-N cell can also cleave the target specifically and cause about 30% reduction of BmNPV polyhedra in transfected cell.
张晓岚,陈农安,陆长德,祁国荣.抗BmNPV即刻早期蛋白三联ribozyme在家蚕细胞中的表达[J].生物化学与生物物理进展,1996,23(1):83-84
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