Rapid semi-dry technique by filter strips shortens running time from 2～4h to 40～50 min without lowering resolution and using large amount of electrode buffer.It also simplifies operation and saves materials. Being compared with pH 8.9 anode PAGE, pH 4.8 PAGE has higher resolution in acid protein seperation.Very good results can be obtained by using pH 5.5 cathode PAGE with semi-dry technique by filter strips for alkaline sample separations.