蛋白质与蛋白质的相互作用是生物体内包括复制、转录、分泌、信号传导、代谢等生命活动得以进行的物质基础.以基因工程技术为基础的双杂合系统可以在体内检测蛋白质与蛋白质的相互作用,并推广应用到寻找同某已知蛋白质相互作用的未知蛋白质,直接克隆未知蛋白质的基因,鉴别同已知蛋白质相互作用的关键氨基酸残基,绘制蛋白质联系图谱等.双杂合系统在药物设计中的应用使其具有更重大的实用价值.
Protein-protein interaction is the basis of many phenomena including replication,transcription, secretion, signal transduction,metabolism et al. Designed genetically,twohybrid system can be used to detect protein-protein interaction in vivo. An unknown protein which interacts with certain target protein can also be found by using this method.Consequently, the DNA sequence encoding the unknown protein can be cloned,the key amino acids involved in the interaction can also be decided. Drawing protein linkage map is its another application. Being used in drug design, two-hybrid system will have a more practical value in future.
黄家学,马晓军,刘汀.双杂合系统及其应用[J].生物化学与生物物理进展,1996,23(6):505-508
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