改进了一种分析磷酸酶活性的终止酶反应方法.该方法通过在酶反应进行到一定程度时,在反应混合物中加入酶反应终止液(1mol/L NaOH-0.2mol/L EDTA),从而使测定更简捷、精确.
A phosphatase activity assay where p-nitrophenyl phosphate was used as substrate was improved by use of the terminating reagent containing 1 mol/L NaOH-0.2 mol/L EDTA. A better assay result was obtained.
梁智群,李相萍,杨胜远.鼠肝抽提液中磷酸酶活性终止方法的改进[J].生物化学与生物物理进展,1996,23(6):561-563
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