采用PCR技术从rec-M13mp18中扩增出120 bp的大鼠肝tRNAIle合成基因片段，经限制性内切酶BstNⅠ酶切后作为模板，利用T7 RNA聚合酶在体外无细胞体系转录由T7启动子带动的大鼠肝tRNAIle基因，生成不含修饰碱基的tRNAIle,并对体外转录反应条件进行了优化，回收的tRNA产量可达DNA模板量的40倍.
The rat liver tRNAIle gene has been synthesized before. Now the synthetic tRNAIle gene was transcripted in vitro with T7 RNA polymerase and the optimum reaction condition was investigated.
张璐,钟雄霖,彭朝晖,徐钤.用T7 RNA聚合酶体外转录合成大鼠肝tRNAIle[J].生物化学与生物物理进展,1997,24(1):78-82
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