Dynamics in countercurrent chromatography, a kind of liquid-liquid chromatography without solid matrix, is very different to that in other liquid-liquid chromatography. Dynamics in countercurrent chromatography was analyzed and the rate equation that fits countercurrent chromatography was derived out. Some experiments were done to measure H(plate height) of two kinds of CCPC(centrifuge countercurrent partition chromatography) columns. The results of both theoretical deriving and experiments reveal that the diameter of column, capacity factor, mass transfer coefficient, the average diameter and velocity of mobile phase droplets are main factors that determines H. Mathematics analysis is given.